##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547400_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2453331 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.278555156234525 31.0 31.0 33.0 30.0 34.0 2 31.680933799801167 31.0 31.0 34.0 30.0 34.0 3 31.750791474937543 31.0 31.0 34.0 30.0 34.0 4 35.55463857098777 37.0 35.0 37.0 33.0 37.0 5 35.46418889257096 37.0 35.0 37.0 33.0 37.0 6 35.49905944204023 37.0 35.0 37.0 33.0 37.0 7 35.89305193632657 37.0 35.0 37.0 35.0 37.0 8 35.88858535599151 37.0 35.0 37.0 35.0 37.0 9 37.63226405242505 39.0 37.0 39.0 35.0 39.0 10 36.92208267045906 39.0 37.0 39.0 32.0 39.0 11 36.62412572946741 39.0 35.0 39.0 32.0 39.0 12 36.314426386003355 38.0 35.0 39.0 32.0 39.0 13 36.20198456710489 38.0 35.0 39.0 32.0 39.0 14 37.29625639589603 39.0 36.0 41.0 32.0 41.0 15 37.40768938231327 39.0 36.0 41.0 32.0 41.0 16 37.481900322459545 39.0 36.0 41.0 32.0 41.0 17 37.372916251414914 39.0 35.0 41.0 32.0 41.0 18 37.21459395409751 39.0 36.0 41.0 32.0 41.0 19 37.11497755500583 39.0 35.0 41.0 31.0 41.0 20 36.947446960887056 39.0 35.0 41.0 31.0 41.0 21 36.80836911122062 38.0 35.0 41.0 31.0 41.0 22 36.72119294135198 38.0 35.0 40.0 31.0 41.0 23 36.61070764605347 38.0 35.0 40.0 31.0 41.0 24 36.48724733841459 38.0 35.0 40.0 30.0 41.0 25 36.377840169141464 38.0 35.0 40.0 30.0 41.0 26 36.15995966300511 38.0 35.0 40.0 30.0 41.0 27 36.14488179540388 38.0 35.0 40.0 30.0 41.0 28 36.18334990264257 38.0 35.0 40.0 30.0 41.0 29 36.18578047560643 38.0 35.0 40.0 30.0 41.0 30 36.16140789807817 38.0 35.0 40.0 30.0 41.0 31 36.09203731579636 38.0 35.0 40.0 30.0 41.0 32 35.90239474412544 38.0 35.0 40.0 29.0 41.0 33 35.66241734197302 38.0 34.0 40.0 27.0 41.0 34 35.4067160118223 38.0 34.0 40.0 26.0 41.0 35 35.22698037892155 38.0 34.0 40.0 25.0 41.0 36 34.98981181096232 38.0 34.0 40.0 24.0 41.0 37 34.95819602002339 38.0 34.0 40.0 24.0 41.0 38 34.8623218799257 38.0 34.0 40.0 24.0 41.0 39 34.71297024331409 38.0 34.0 40.0 23.0 41.0 40 34.56879768771519 38.0 33.0 40.0 23.0 41.0 41 34.52711476763633 37.0 33.0 40.0 23.0 41.0 42 34.44288072013112 37.0 33.0 40.0 23.0 41.0 43 34.32485791766378 37.0 33.0 40.0 23.0 41.0 44 34.22865157616318 37.0 33.0 40.0 23.0 41.0 45 34.068788108901735 37.0 33.0 40.0 23.0 41.0 46 33.965554994413715 36.0 33.0 40.0 23.0 41.0 47 33.91967940730378 36.0 33.0 40.0 23.0 41.0 48 33.79541203367992 36.0 33.0 40.0 22.0 41.0 49 33.63868308026923 36.0 32.0 40.0 22.0 41.0 50 33.52568650540836 36.0 32.0 40.0 21.0 41.0 51 33.041773816904445 35.0 31.0 39.0 20.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 5.0 12 3.0 13 13.0 14 26.0 15 105.0 16 236.0 17 665.0 18 1377.0 19 2621.0 20 4648.0 21 7331.0 22 10553.0 23 15231.0 24 22078.0 25 31964.0 26 43804.0 27 52461.0 28 57155.0 29 61057.0 30 69193.0 31 81959.0 32 97601.0 33 122514.0 34 180264.0 35 222472.0 36 207358.0 37 259860.0 38 366399.0 39 534231.0 40 144.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.887653561627026 23.079356189605072 27.566643066100742 16.466347182667157 2 33.11738204098835 24.818094256339645 28.350556855149183 13.71396684752282 3 28.41080147766445 25.748828837201337 32.63012614278302 13.210243542351193 4 24.722387643575207 28.291168211708893 32.387109607305334 14.599334537410567 5 22.484165406135574 32.56409347128455 30.73792325617701 14.213817866402861 6 22.126040065527235 40.17187244607434 27.724061694080415 9.978025794318011 7 88.28266548623075 2.501415422541842 8.057004945521008 1.1589141457063885 8 88.60875275288984 2.2695266150389 7.881162386975096 1.240558245096157 9 83.8266014655177 4.810561640479821 9.135008688187611 2.2278282058148697 10 38.853501626971656 35.02544092093566 15.200884022579913 10.920173429512772 11 30.0402595491599 25.83886968370758 27.64233607287398 16.47853469425854 12 28.695271856916165 22.289532068848438 30.085667201042178 18.92952887319322 13 23.733813333789854 25.592673797379973 31.083371954293977 19.590140914536196 14 20.53159561428931 26.9017918902912 31.83708191026812 20.72953058515137 15 19.920508076570183 25.581342264863565 35.29992487764594 19.19822478092031 16 23.804900357921536 24.3727405719 33.47603727340502 18.34632179677345 17 23.38604126389794 24.62117830818589 30.407556094143022 21.58522433377314 18 24.00797120323348 24.33344705626758 31.70342689184623 19.955154848652707 19 25.889943101848058 25.849630563507333 28.691603375166252 19.568822959478357 20 28.164972439511832 25.189915262147665 29.04300316589975 17.602109132440752 21 26.483748014434255 24.97433081797768 30.064226963259337 18.477694204328728 22 26.231560274581785 23.055266492780632 29.510693828105545 21.202479404532042 23 23.739234534598065 25.488570437499057 30.58095299818899 20.191242029713887 24 22.785021670536914 24.337278581650825 33.30651265565062 19.571187092161637 25 22.941502797624942 26.145840084358774 30.558289933156185 20.354367184860095 26 22.930945722366854 27.602716469974904 28.86084266656232 20.605495141095922 27 21.444966048201405 27.274183548815877 30.647067191504124 20.633783211478598 28 20.645563113986658 27.31152054084834 31.943304837382318 20.099611507782683 29 21.37872957216128 25.66429071331997 31.641837159355994 21.315142555162755 30 22.426244155395256 25.908693119680958 31.262271580964818 20.402791143958968 31 24.103188685097933 26.03232095465308 29.032649895183326 20.83184046506566 32 24.91587967542904 25.628747201254132 28.970122661801444 20.485250461515385 33 24.193229531604175 26.305011431396743 28.923329139035864 20.57842989796322 34 22.296257618723278 26.955147919298295 30.12467539031627 20.62391907166216 35 22.44951863405305 27.184305745942964 30.02941714754348 20.336758472460502 36 24.203256715053943 28.22578771474375 27.464944599811442 20.10601097039087 37 23.082087170463343 28.922147072694226 27.862118890602204 20.133646866240227 38 22.820239095336095 28.574293480985645 28.098980528921697 20.506486894756556 39 23.06786161345534 28.45388575777178 27.328517839622947 21.149734789149935 40 24.9232573998372 27.318246090723186 27.834849842927838 19.923646666511775 41 21.269531098738817 26.872892406283537 29.070435257207443 22.787141237770197 42 22.29165163608172 27.13738994045239 27.735352465688486 22.835605957777403 43 22.43949145060328 26.70271561399583 28.37472807379029 22.4830648616106 44 21.996827986113573 27.346982531097513 28.71169850297412 21.94449097981479 45 21.889993645374393 27.624482795024395 27.675515452256544 22.810008107344668 46 21.689613020012384 28.348681853365893 27.677961106756488 22.28374401986524 47 21.78177343375191 27.70274373902258 29.282881111435838 21.232601715789677 48 22.871964688009893 26.597185622323284 29.55035419191295 20.98049549775387 49 22.414790340153857 27.156955176451937 29.080014070665555 21.348240412728654 50 21.45870247430942 28.166888202203456 29.300122975660436 21.07428634782669 51 21.39291436826095 27.51707780156856 28.474429255571305 22.615578574599187 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1119.0 1 2281.0 2 3443.0 3 22597.5 4 41752.0 5 28819.5 6 15887.0 7 15472.0 8 15057.0 9 15504.5 10 15952.0 11 15952.0 12 15952.0 13 15704.5 14 15457.0 15 14753.5 16 14050.0 17 13724.5 18 13399.0 19 13155.5 20 12912.0 21 13541.5 22 14171.0 23 14888.0 24 15605.0 25 17490.0 26 23384.0 27 27393.0 28 33377.5 29 39362.0 30 43979.0 31 48596.0 32 54611.5 33 60627.0 34 68951.0 35 77275.0 36 84413.0 37 91551.0 38 96785.5 39 102020.0 40 112555.5 41 123091.0 42 134096.0 43 145101.0 44 148411.0 45 151721.0 46 153105.0 47 154489.0 48 154947.5 49 155406.0 50 154137.0 51 152868.0 52 149341.0 53 145814.0 54 142262.5 55 138711.0 56 132686.5 57 126662.0 58 121375.0 59 116088.0 60 110947.5 61 105807.0 62 94339.5 63 82872.0 64 71686.5 65 60501.0 66 52296.5 67 44092.0 68 37101.0 69 30110.0 70 25570.5 71 21031.0 72 18040.0 73 15049.0 74 12746.0 75 8481.5 76 6520.0 77 4874.0 78 3228.0 79 2350.0 80 1472.0 81 1094.5 82 717.0 83 533.5 84 350.0 85 229.0 86 108.0 87 85.0 88 62.0 89 50.0 90 38.0 91 26.5 92 15.0 93 10.5 94 6.0 95 5.0 96 4.0 97 2.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2453331.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.987182314863258 #Duplication Level Percentage of deduplicated Percentage of total 1 76.29260701152785 17.537520666502054 2 5.28756134314812 2.430922731919382 3 2.2047442651275397 1.5204257514040793 4 1.4074383887015791 1.2941217135208232 5 1.1348861509774613 1.3043917429566165 6 0.8927349963037209 1.2312877271335443 7 0.8020194834498423 1.2905317660293787 8 0.6930529219923323 1.2745067097349154 9 0.6180818638741677 1.2787164441347387 >10 9.472106802359082 50.7541768020194 >50 1.1396706523141682 16.827024425825265 >100 0.05312859691486369 1.8679206525320797 >500 0.0014309260431094298 0.19456184557653045 >1k 3.5773151077735744E-4 0.11648218692271994 >5k 0.0 0.0 >10k+ 1.7886575538867872E-4 1.0774088337885468 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26204 1.0680988419418334 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2228272499715693E-4 0.0 0.0 0.014388600641332131 0.0 2 1.2228272499715693E-4 0.0 0.0 0.042635910115675385 0.0 3 1.2228272499715693E-4 0.0 0.0 0.06468756152349601 0.0 4 1.2228272499715693E-4 0.0 0.0 0.10809792889748672 0.0 5 1.2228272499715693E-4 0.0 0.0 0.18896757102893982 0.0 6 1.2228272499715693E-4 0.0 0.0 0.3745927475746241 0.0 7 1.2228272499715693E-4 0.0 0.0 0.46455207226419915 0.0 8 1.6304363332954257E-4 0.0 0.0 0.6352587563602302 0.0 9 1.6304363332954257E-4 0.0 4.076090833238564E-5 0.7268485173831007 0.0 10 1.6304363332954257E-4 0.0 4.076090833238564E-5 0.8524328759551809 0.0 11 1.6304363332954257E-4 0.0 4.076090833238564E-5 0.9816857162771758 0.0 12 1.6304363332954257E-4 0.0 4.076090833238564E-5 1.073560803658373 0.0 13 1.6304363332954257E-4 0.0 4.076090833238564E-5 1.132215750748676 0.0 14 2.038045416619282E-4 0.0 4.076090833238564E-5 1.168778285522826 0.0 15 2.038045416619282E-4 0.0 4.076090833238564E-5 1.206196799371956 0.0 16 2.038045416619282E-4 0.0 4.076090833238564E-5 1.2593082629290544 0.0 17 2.4456544999431387E-4 0.0 4.076090833238564E-5 1.3218762572192664 0.0 18 2.4456544999431387E-4 0.0 4.076090833238564E-5 1.3933708904342708 0.0 19 2.4456544999431387E-4 0.0 4.076090833238564E-5 1.444974200383071 0.0 20 2.4456544999431387E-4 0.0 4.076090833238564E-5 1.5008574057067718 0.0 21 2.853263583266995E-4 0.0 4.076090833238564E-5 1.5717813862051226 0.0 22 2.853263583266995E-4 0.0 4.076090833238564E-5 1.6526917892449082 0.0 23 2.853263583266995E-4 0.0 4.076090833238564E-5 1.743670136642793 0.0 24 2.853263583266995E-4 0.0 4.076090833238564E-5 1.8174066198160785 0.0 25 2.853263583266995E-4 0.0 4.076090833238564E-5 1.880952875906268 0.0 26 2.853263583266995E-4 0.0 4.076090833238564E-5 1.9436839138298094 0.0 27 2.853263583266995E-4 0.0 4.076090833238564E-5 2.0094720198782796 0.0 28 2.853263583266995E-4 0.0 4.076090833238564E-5 2.081781871259932 0.0 29 2.853263583266995E-4 0.0 4.076090833238564E-5 2.1591460752748 0.0 30 2.853263583266995E-4 0.0 4.076090833238564E-5 2.2517140980976476 0.0 31 2.853263583266995E-4 0.0 4.076090833238564E-5 2.3399614646372626 0.0 32 2.853263583266995E-4 0.0 4.076090833238564E-5 2.440192538226599 0.0 33 2.853263583266995E-4 0.0 4.076090833238564E-5 2.533005126499441 0.0 34 2.853263583266995E-4 0.0 4.076090833238564E-5 2.61978510033909 0.0 35 2.853263583266995E-4 0.0 4.076090833238564E-5 2.721972697528381 0.0 36 2.853263583266995E-4 0.0 4.076090833238564E-5 2.8101385422513308 0.0 37 2.853263583266995E-4 0.0 4.076090833238564E-5 2.909921245849011 0.0 38 2.853263583266995E-4 0.0 4.076090833238564E-5 3.0213615692297533 0.0 39 2.853263583266995E-4 0.001385870883301112 4.076090833238564E-5 3.1740111709345378 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTTT 14375 0.0 42.18261 1 CGTAAGG 425 0.0 40.764706 2 CACGACC 1245 0.0 40.48193 27 ACGACCA 1270 0.0 39.15354 28 GGGCGAT 4365 0.0 38.969074 7 AACACGT 1205 0.0 38.65145 41 CGACCAA 1285 0.0 38.5214 29 CGGGTAT 300 0.0 38.25 6 GGCGATA 950 0.0 38.131577 8 TAGGGAT 3510 0.0 38.01282 5 TACGGGA 990 0.0 37.954544 4 TTTAGCG 185 0.0 37.7027 1 TAAGGGA 3570 0.0 37.5 4 ACACGAC 1330 0.0 37.21805 26 AGGGCGA 2515 0.0 37.12724 6 CGAGACA 1330 0.0 37.048874 22 GCGATAC 110 0.0 36.81818 9 TATAGCG 110 0.0 36.81818 1 GCGAGAC 1345 0.0 36.802975 21 GACACGA 1340 0.0 36.772385 25 >>END_MODULE