FastQCFastQC Report
Thu 26 May 2016
SRR1547399_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547399_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1191236
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169691.4244868355220965No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20580.1727617365492648No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCACG207.0333906E-445.035
CTATCGG502.1827873E-1145.02
CGGTTCG207.0333906E-445.044
CCCGTAT207.0333906E-445.028
TAACCGC207.0333906E-445.012
TACGATA207.0333906E-445.036
CGTAGCG207.0333906E-445.01
CGGTGAA207.0333906E-445.06
GTATCGT207.0333906E-445.025
TTACGCT207.0333906E-445.027
CGGTCTA700.045.031
ACGTAAC253.890892E-545.028
CGGTCGA207.0333906E-445.025
CCGCGAA207.0333906E-445.036
TAGTGCG551.8189894E-1245.01
TATCGTA207.0333906E-445.026
GCGTAAG351.2119744E-745.01
TCGATAG650.044.9999961
TCTAGCG302.1655687E-644.9999961
CCCGATA302.1655687E-644.99999644