FastQCFastQC Report
Thu 26 May 2016
SRR1547398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547398_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2740653
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280511.023515198750079No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT124300.042.8640371
TTTAGCG2650.041.6037751
ACGATTG1600.040.781251
TAGGGCG7450.040.7718125
TACGGGA12200.040.3893434
AAGGGAT61000.039.0614745
TAGGGAT55650.038.8948825
GCGCGAC2500.038.6999979
ATAGGGA43200.038.5937544
CGTTAGG3850.038.571432
CTATACG700.038.5714261
ACCGTAT356.2514555E-638.57142632
AGGGATT67550.038.4381946
ACGGGAT15350.038.4039085
AGGGATC42500.038.382356
GGGCGAT44600.038.340817
TACGCGG950.037.8947332
GCGATGC3450.037.8260849
ACGGGAC12050.037.531125
AGGGCGA19250.037.519486
Produced by FastQC (version 0.11.5)