##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547398_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2740653 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37088460304898 31.0 31.0 33.0 30.0 34.0 2 31.777642773455813 31.0 31.0 34.0 30.0 34.0 3 31.8488955004519 31.0 31.0 34.0 30.0 34.0 4 35.63329505778368 37.0 35.0 37.0 33.0 37.0 5 35.53992716334392 37.0 35.0 37.0 33.0 37.0 6 35.57280582401347 37.0 35.0 37.0 33.0 37.0 7 35.99330743439611 37.0 35.0 37.0 35.0 37.0 8 36.00752010560987 37.0 35.0 37.0 35.0 37.0 9 37.765532520899214 39.0 38.0 39.0 35.0 39.0 10 37.06198267347234 39.0 37.0 39.0 33.0 39.0 11 36.714616553062356 39.0 37.0 39.0 32.0 39.0 12 36.492987984980225 39.0 35.0 39.0 32.0 39.0 13 36.37246743750486 39.0 35.0 39.0 32.0 39.0 14 37.47692684918521 40.0 36.0 41.0 32.0 41.0 15 37.60515760295083 40.0 36.0 41.0 32.0 41.0 16 37.683451352652085 40.0 36.0 41.0 33.0 41.0 17 37.588176248507196 39.0 36.0 41.0 32.0 41.0 18 37.453920653216585 39.0 36.0 41.0 32.0 41.0 19 37.40687018750641 39.0 36.0 41.0 32.0 41.0 20 37.26055359799289 39.0 35.0 41.0 32.0 41.0 21 37.13712534932368 39.0 35.0 41.0 32.0 41.0 22 37.05702290658467 39.0 35.0 41.0 32.0 41.0 23 36.96828566038823 39.0 35.0 41.0 31.0 41.0 24 36.88465413169781 39.0 35.0 41.0 31.0 41.0 25 36.79673347921098 38.0 35.0 40.0 31.0 41.0 26 36.60148986391199 38.0 35.0 40.0 31.0 41.0 27 36.60420673467236 38.0 35.0 40.0 31.0 41.0 28 36.64888002968635 38.0 35.0 40.0 31.0 41.0 29 36.6566084798039 38.0 35.0 40.0 31.0 41.0 30 36.635683539652774 38.0 35.0 40.0 31.0 41.0 31 36.55957503558459 38.0 35.0 41.0 30.0 41.0 32 36.399066207943875 38.0 35.0 41.0 30.0 41.0 33 36.2379305223974 38.0 35.0 41.0 30.0 41.0 34 36.05716776257337 38.0 35.0 41.0 29.0 41.0 35 35.934399210699056 38.0 35.0 41.0 29.0 41.0 36 35.73623256939131 38.0 35.0 40.0 28.0 41.0 37 35.69751661374132 38.0 35.0 40.0 27.0 41.0 38 35.632527722407765 38.0 35.0 40.0 27.0 41.0 39 35.51181306061001 38.0 35.0 40.0 27.0 41.0 40 35.407340513374 38.0 34.0 40.0 26.0 41.0 41 35.382709522146726 38.0 34.0 40.0 26.0 41.0 42 35.30681410598131 38.0 34.0 40.0 26.0 41.0 43 35.21701944755502 38.0 34.0 40.0 26.0 41.0 44 35.12985518414772 38.0 34.0 40.0 25.0 41.0 45 34.9848072703841 38.0 34.0 40.0 24.0 41.0 46 34.921779590484455 38.0 34.0 40.0 24.0 41.0 47 34.885646960779056 38.0 34.0 40.0 24.0 41.0 48 34.784153630539876 38.0 34.0 40.0 24.0 41.0 49 34.67177566806159 37.0 34.0 40.0 24.0 41.0 50 34.56909247540641 37.0 33.0 40.0 24.0 41.0 51 34.10303019025028 37.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 4.0 13 2.0 14 23.0 15 58.0 16 170.0 17 433.0 18 981.0 19 1961.0 20 3231.0 21 5503.0 22 8585.0 23 12533.0 24 19021.0 25 27941.0 26 38328.0 27 45967.0 28 50674.0 29 56937.0 30 67405.0 31 81169.0 32 100752.0 33 128941.0 34 192984.0 35 255629.0 36 224619.0 37 282992.0 38 420561.0 39 713029.0 40 218.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.169104589307732 24.089769846821177 29.16097003159466 15.580155532276432 2 31.039536927878135 25.65574700627916 29.862737092218534 13.441978973624169 3 28.016206356660255 25.798450223359175 32.894861188191285 13.290482231789285 4 24.82123785827684 28.177445302269206 32.425301561343225 14.576015278110727 5 21.954439325226506 33.0396806892372 31.02928389693989 13.976596088596406 6 21.938494220173073 41.11191748827743 27.301522666313467 9.648065625236029 7 89.80191946955706 2.663817710596708 6.5655520782820735 0.9687107415641456 8 90.52225874636446 1.8413130009526928 6.322033471585057 1.3143947810977896 9 85.6408673407396 5.0224891659031625 7.589541616541751 1.747101876815489 10 38.3322514743749 39.80919875664668 13.069731921553002 8.788817847425413 11 25.220376311776793 25.677019308901926 31.791656951828635 17.310947427492646 12 26.527619512576017 24.38542931191946 31.142140212569778 17.944810962934746 13 22.294613728917888 28.19806082710945 31.810703507521747 17.696621936450914 14 18.086492525686396 31.429444004768207 31.79468542715915 18.68937804238625 15 17.55388223171631 28.59789254604651 36.97969060658172 16.868534615655467 16 21.236252820039603 26.39235977703124 35.98846698213893 16.382920420790228 17 21.007767127031403 26.310189578906922 31.28539074446856 21.39665254959311 18 21.220490153259096 27.0006454666096 32.99549413953536 18.783370240595946 19 23.36913866877711 28.385753322292167 29.88641758004388 18.358690428886838 20 25.25978297872806 28.426984371972665 29.775020770597372 16.5382118787019 21 23.669359090698457 26.813135409699807 32.27847523929516 17.239030260306578 22 22.669852768664985 25.035785267233756 31.721345241444283 20.573016722656973 23 19.703625376871862 28.87034586282904 31.63092883338387 19.795099926915228 24 19.92061745868594 27.344395660450267 34.64834840455906 18.086638476304735 25 20.037377953356373 29.406276533366317 31.74422300086877 18.81212251240854 26 19.585441863672635 31.9380089343671 29.44269121264166 19.033857989318605 27 18.700214875797847 31.325855553402782 31.537994777157124 18.435934793642243 28 18.070474445323796 29.87404826513973 34.16649243811602 17.888984851420446 29 18.379378929036257 27.81417421322583 34.027000134639444 19.779446723098474 30 19.584456696998853 28.749462263190562 32.71063502019409 18.955446019616492 31 22.219339697510048 28.516853465214314 30.362107132862132 18.90169970441351 32 22.55119491595616 28.430888551013204 30.701478808152654 18.316437724877975 33 21.830308324330005 28.527653810971326 30.492732936274674 19.149304928423994 34 19.522938511369368 28.59183559538548 31.573059413212835 20.312166480032314 35 19.391801880792645 28.32273914282472 31.657236432339296 20.628222544043336 36 22.17073814160348 28.156245974955603 30.237027452946435 19.435988430494483 37 19.643055870261577 29.936369179170075 31.158632632441975 19.261942318126373 38 19.694248049643644 30.730705419474845 29.502166089614406 20.0728804412671 39 19.540306634951598 29.50647163285538 30.197511323031407 20.755710409161612 40 21.293939801937714 28.860238782509136 29.680298819295984 20.16552259625717 41 18.67514785709829 28.068639116298193 30.845495580797717 22.4107174458058 42 19.797435136808637 29.241279359335167 29.667126775990976 21.29415872786522 43 20.519708259309006 28.666963676174984 29.73017744311301 21.083150621403 44 19.63440829612505 29.66676189944513 30.261109304972212 20.437720499457612 45 19.243662003179534 30.685825604335903 29.312758674666224 20.757753717818346 46 20.54992003730498 30.09793651367028 29.109595413939672 20.24254803508507 47 19.566723696870785 29.241060433407657 30.549471239153586 20.64274463056797 48 20.288230578624873 28.5573912494577 31.36920288704918 19.78517528486824 49 20.50431046907434 28.065647128622267 30.841117062247577 20.58892534005582 50 19.04571647705857 30.15799519311638 30.632553628642516 20.163734701182527 51 18.622934023387856 30.68137411047659 29.411275342044398 21.284416524091156 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1384.0 1 2291.5 2 3199.0 3 22159.0 4 41119.0 5 27355.5 6 13592.0 7 13880.0 8 14168.0 9 15212.5 10 16257.0 11 16852.0 12 17447.0 13 17904.5 14 18362.0 15 17999.0 16 17636.0 17 17313.0 18 16990.0 19 16814.0 20 16638.0 21 17533.5 22 18429.0 23 21009.0 24 23589.0 25 27658.5 26 36325.0 27 40922.0 28 49167.5 29 57413.0 30 64935.0 31 72457.0 32 83500.5 33 94544.0 34 106992.0 35 119440.0 36 123174.5 37 126909.0 38 138145.5 39 149382.0 40 168126.0 41 186870.0 42 198526.5 43 210183.0 44 212366.5 45 214550.0 46 214248.5 47 213947.0 48 208952.5 49 203958.0 50 194920.5 51 185883.0 52 167061.0 53 148239.0 54 135405.5 55 122572.0 56 108008.0 57 93444.0 58 86784.5 59 80125.0 60 70058.5 61 59992.0 62 51870.5 63 43749.0 64 35345.0 65 26941.0 66 21686.5 67 16432.0 68 12555.0 69 8678.0 70 6889.5 71 5101.0 72 4211.0 73 3321.0 74 2854.0 75 1893.0 76 1399.0 77 1020.0 78 641.0 79 472.0 80 303.0 81 215.0 82 127.0 83 137.0 84 147.0 85 88.5 86 30.0 87 24.0 88 18.0 89 12.0 90 6.0 91 4.0 92 2.0 93 2.0 94 2.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2740653.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.058735056439577 #Duplication Level Percentage of deduplicated Percentage of total 1 78.40379626929086 16.510847730540622 2 6.958566091568195 2.9307719939011783 3 2.603032770073564 1.6444973234462756 4 1.5468228672746935 1.302965317647199 5 1.0133106737895963 1.0669520504598693 6 0.7243626481978018 0.9152496655907076 7 0.5543694172621985 0.8172023077062208 8 0.43483803029003054 0.7325711097873441 9 0.3627833866297151 0.6875783299721749 >10 4.94054962664821 24.851097097817505 >50 1.6821699108247223 25.33028031637043 >100 0.7729462144264071 21.77474565151059 >500 0.00210179462060074 0.3419910446103174 >1k 1.7514955171672836E-4 0.05860912099717367 >5k 0.0 0.0 >10k+ 1.7514955171672836E-4 1.0346409396423653 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28051 1.023515198750079 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.648765458450961E-5 0.0 0.0 0.03809311138622803 0.0 2 3.648765458450961E-5 0.0 0.0 0.1151185502141278 0.0 3 3.648765458450961E-5 0.0 0.0 0.16532556292241302 0.0 4 3.648765458450961E-5 0.0 0.0 0.2492471684667851 0.0 5 3.648765458450961E-5 0.0 0.0 0.43781536735953075 0.0 6 3.648765458450961E-5 0.0 0.0 0.7768586537587939 0.0 7 3.648765458450961E-5 0.0 0.0 0.9682364020545469 0.0 8 3.648765458450961E-5 0.0 0.0 1.3688708493924624 0.0 9 3.648765458450961E-5 0.0 0.0 1.5780910607800405 0.0 10 3.648765458450961E-5 0.0 0.0 1.8579878590978136 0.0 11 3.648765458450961E-5 0.0 0.0 2.167986972447807 0.0 12 3.648765458450961E-5 0.0 0.0 2.432121103985072 0.0 13 3.648765458450961E-5 0.0 0.0 2.596570963197457 0.0 14 3.648765458450961E-5 0.0 0.0 2.7019473096375206 0.0 15 3.648765458450961E-5 0.0 0.0 2.794844878209682 0.0 16 3.648765458450961E-5 0.0 0.0 2.9280613050977267 0.0 17 3.648765458450961E-5 0.0 0.0 3.09783836187945 0.0 18 3.648765458450961E-5 0.0 0.0 3.281663165676209 0.0 19 3.648765458450961E-5 0.0 0.0 3.414806617255085 0.0 20 3.648765458450961E-5 0.0 0.0 3.544082377448002 0.0 21 3.648765458450961E-5 0.0 0.0 3.6958710205195624 0.0 22 3.648765458450961E-5 0.0 0.0 3.873711848964462 0.0 23 3.648765458450961E-5 0.0 0.0 4.057828553997898 0.0 24 3.648765458450961E-5 0.0 0.0 4.2046183883913795 0.0 25 3.648765458450961E-5 0.0 0.0 4.335171216494755 0.0 26 3.648765458450961E-5 0.0 0.0 4.461600939630081 0.0 27 3.648765458450961E-5 0.0 0.0 4.598283693703654 0.0 28 3.648765458450961E-5 0.0 0.0 4.7350759107409806 0.0 29 3.648765458450961E-5 0.0 0.0 4.890221418034315 0.0 30 3.648765458450961E-5 0.0 0.0 5.085320907097688 0.0 31 3.648765458450961E-5 0.0 0.0 5.2530546552226784 0.0 32 3.648765458450961E-5 0.0 0.0 5.424218242878613 0.0 33 3.648765458450961E-5 0.0 0.0 5.589470830491857 0.0 34 3.648765458450961E-5 0.0 0.0 5.754431516868425 0.0 35 3.648765458450961E-5 0.0 0.0 5.942853765142833 0.0 36 3.648765458450961E-5 0.0 0.0 6.116316075037592 0.0 37 3.648765458450961E-5 0.0 0.0 6.292186570134928 0.0 38 3.648765458450961E-5 0.0 0.0 6.4899861456375545 0.0 39 3.648765458450961E-5 0.0 0.0 6.7483917154050514 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTTT 12430 0.0 42.864037 1 TTTAGCG 265 0.0 41.603775 1 ACGATTG 160 0.0 40.78125 1 TAGGGCG 745 0.0 40.771812 5 TACGGGA 1220 0.0 40.389343 4 AAGGGAT 6100 0.0 39.061474 5 TAGGGAT 5565 0.0 38.894882 5 GCGCGAC 250 0.0 38.699997 9 ATAGGGA 4320 0.0 38.593754 4 CGTTAGG 385 0.0 38.57143 2 CTATACG 70 0.0 38.571426 1 ACCGTAT 35 6.2514555E-6 38.571426 32 AGGGATT 6755 0.0 38.438194 6 ACGGGAT 1535 0.0 38.403908 5 AGGGATC 4250 0.0 38.38235 6 GGGCGAT 4460 0.0 38.34081 7 TACGCGG 95 0.0 37.894733 2 GCGATGC 345 0.0 37.826084 9 ACGGGAC 1205 0.0 37.53112 5 AGGGCGA 1925 0.0 37.51948 6 >>END_MODULE