FastQCFastQC Report
Thu 26 May 2016
SRR1547397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547397_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1781882
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175780.9864850758916697No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32610.18300875142125012No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18210.10219531933090967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATA302.1661763E-645.00000425
CGGGTAC453.8562575E-1045.06
CGAAAGT253.891673E-545.045
GATCACG700.045.01
TACGCTA207.0343324E-445.015
TACGCGA207.0343324E-445.037
CGAATGT207.0343324E-445.044
ACTCGTT207.0343324E-445.033
CATACGT207.0343324E-445.023
TTACGTC453.8562575E-1045.029
CGTAACA253.891673E-545.040
ACGTAAG950.045.01
GCGTACA253.891673E-545.037
CGTTAGG2150.042.9069752
CGTTTTT92400.042.1266251
GCGATAT2250.042.09
GTATGCG700.041.7857131
TTTGACG1300.041.5384641
CGAAAGG2450.041.326532
CTAACGG603.6379788E-1241.2500042