##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547397_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1781882
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.337934835191106	31.0	31.0	33.0	30.0	34.0
2	31.737584194688537	31.0	31.0	34.0	30.0	34.0
3	31.808361608681157	31.0	31.0	34.0	30.0	34.0
4	35.57999182886409	37.0	35.0	37.0	33.0	37.0
5	35.4781113451957	37.0	35.0	37.0	33.0	37.0
6	35.51263495562557	37.0	35.0	37.0	33.0	37.0
7	35.92041897274904	37.0	35.0	37.0	35.0	37.0
8	35.92116649699587	37.0	35.0	37.0	35.0	37.0
9	37.67745058314748	39.0	37.0	39.0	35.0	39.0
10	37.00950006790573	39.0	37.0	39.0	33.0	39.0
11	36.665790439546505	39.0	35.0	39.0	32.0	39.0
12	35.80137405282729	37.0	35.0	39.0	31.0	39.0
13	35.30017756506884	37.0	35.0	39.0	30.0	39.0
14	36.33096130944698	38.0	35.0	41.0	30.0	41.0
15	36.69585247507972	38.0	35.0	41.0	31.0	41.0
16	36.91923090305643	38.0	35.0	41.0	32.0	41.0
17	36.856768293298884	38.0	35.0	41.0	32.0	41.0
18	36.75690814543275	38.0	35.0	40.0	32.0	41.0
19	36.63984090977966	38.0	35.0	40.0	31.0	41.0
20	36.43574827064867	38.0	35.0	40.0	31.0	41.0
21	36.26312853488615	37.0	35.0	40.0	31.0	41.0
22	36.14018717288799	37.0	35.0	40.0	30.0	41.0
23	36.101414684025094	37.0	35.0	40.0	31.0	41.0
24	35.96973480847778	37.0	34.0	40.0	30.0	41.0
25	35.88199162458569	37.0	34.0	40.0	30.0	41.0
26	35.61143835562624	36.0	34.0	40.0	30.0	41.0
27	35.55913803495405	36.0	34.0	40.0	30.0	41.0
28	35.62651455034621	36.0	34.0	40.0	30.0	41.0
29	35.7031178271064	36.0	34.0	40.0	30.0	41.0
30	35.72772158874718	36.0	35.0	40.0	30.0	41.0
31	35.59011315002901	36.0	34.0	40.0	30.0	41.0
32	35.357371588017614	36.0	34.0	40.0	29.0	41.0
33	35.13278488699027	36.0	34.0	40.0	27.0	41.0
34	34.92285347739076	36.0	34.0	40.0	27.0	41.0
35	34.773271181817876	36.0	34.0	40.0	26.0	41.0
36	34.488422914648666	36.0	33.0	40.0	24.0	41.0
37	34.40536298138709	36.0	33.0	40.0	24.0	41.0
38	34.351363895027845	36.0	33.0	40.0	24.0	41.0
39	34.27992538226437	36.0	33.0	40.0	23.0	41.0
40	34.10576794647457	36.0	33.0	40.0	23.0	41.0
41	34.11293733255064	35.0	33.0	40.0	23.0	41.0
42	34.023427477240354	35.0	33.0	40.0	23.0	41.0
43	33.91573740573169	35.0	33.0	40.0	23.0	41.0
44	33.84082616020589	35.0	33.0	40.0	23.0	41.0
45	33.685626208693954	35.0	33.0	40.0	23.0	41.0
46	33.63111418152268	35.0	33.0	40.0	23.0	41.0
47	33.60076705415959	35.0	33.0	40.0	23.0	41.0
48	33.506341609601535	35.0	33.0	39.0	23.0	41.0
49	33.41759162503465	35.0	33.0	39.0	22.0	41.0
50	33.27587741500279	35.0	32.0	39.0	22.0	41.0
51	32.79316251019989	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	5.0
14	33.0
15	71.0
16	220.0
17	493.0
18	1147.0
19	2140.0
20	3727.0
21	5691.0
22	8657.0
23	12125.0
24	17323.0
25	24343.0
26	32952.0
27	39559.0
28	42797.0
29	46627.0
30	53198.0
31	62713.0
32	76704.0
33	98385.0
34	168600.0
35	251982.0
36	126451.0
37	148464.0
38	211978.0
39	345342.0
40	149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.45891927748302	24.40599321391652	29.615709682234854	15.519377826365607
2	30.49264766129295	26.619776169241284	29.9023167639608	12.985259405504964
3	28.272635337244555	25.123493025913053	33.15253198584418	13.451339650998214
4	24.452797660002176	28.412599711989905	32.14859345343856	14.986009174569359
5	22.082943763952944	33.209325870063225	31.206836367391332	13.5008939985925
6	21.3028135420864	41.420251172636576	27.682921764740875	9.594013520536153
7	87.76523922459512	3.572290421026757	7.413790587704461	1.248679766673663
8	88.57292458198691	2.6510734156358278	7.096036662360358	1.6799653400169035
9	84.3686618979259	5.06475737450628	8.40886209075573	2.1577186368120898
10	48.45719301278087	28.74864890043224	13.272147089425676	9.522010997361217
11	43.353768655836916	20.777919076571848	22.8635229493311	13.004789318260132
12	40.57726605914421	20.012997493661196	24.77891353075007	14.630822916444522
13	21.604068058378726	36.78240197723531	25.607756293626625	16.005773670759343
14	15.626455623885308	39.1922136258181	28.087157286509434	17.094173463787165
15	14.438610413035208	25.044531568308116	45.850118021283116	14.666739997373563
16	17.179252049237828	19.947504941404652	46.10361404402761	16.769628965329915
17	17.446553699964422	20.527004594019132	30.10075863609375	31.9256830699227
18	20.925291349258817	23.824136502866068	34.314674035654434	20.935898112220674
19	28.044617993784097	25.263008437146794	26.43048192865745	20.261891640411655
20	30.861246704327222	22.978345367426126	28.115385867302102	18.04502206094455
21	22.744940461826317	28.008813153732966	28.8785115961663	20.36773478827442
22	24.24352454315157	24.807703315932255	26.62471476786903	24.324057373047147
23	21.734828681136012	29.370687845772053	26.19971468368837	22.694768789403565
24	20.64951551225053	23.465807500159944	38.23698763442248	17.647689353167046
25	19.027915428743317	24.964503822363096	35.42142521221944	20.586155536674145
26	18.306880029092838	33.499300178126276	27.785958890656058	20.407860902124835
27	19.683177673942495	34.192331478739895	28.185031332041067	17.939459515276546
28	16.714967657791032	30.04705137601704	36.61774460935124	16.62023635684069
29	16.447834368381294	26.118676769842224	35.83985920504276	21.59362965673372
30	18.85573792203973	29.997272546666952	31.03847505053646	20.108514480756863
31	26.638127552778467	28.128293568260975	26.130518182461017	19.103060696499544
32	28.461368373438873	27.19590859551867	27.418482256400818	16.924240774641643
33	26.206168534167805	28.303445458229	26.309710744033556	19.18067526356964
34	19.8457024651464	28.618617843381323	28.46344482968008	23.072234861792197
35	21.170593787916374	25.94997873035364	29.850012514857887	23.029414966872107
36	28.210173288691394	27.19321481444899	25.038021597389726	19.558590299469884
37	21.32251181615842	31.779152603820005	28.975431594235758	17.922903985785815
38	20.326822988278686	31.89088839777269	25.467511316686515	22.314777297262108
39	21.68847319856197	30.67307487252242	27.621469884088846	20.01698204482676
40	24.002206655659577	27.018736369748392	25.95424388371396	23.02481309087807
41	17.253667751287683	25.683350524894465	29.8919906031937	27.170991120624148
42	21.274584961293733	26.422961789837935	26.43934895801181	25.86310429085652
43	21.708395954389797	26.594914814785714	27.55878335377988	24.13790587704461
44	20.5345808532776	29.806799776865134	28.249120873323825	21.40949849653344
45	18.721946795579054	34.66778383753806	23.92840827843819	22.681861088444688
46	21.526621852625482	32.64610114474472	25.435017582533526	20.39225942009628
47	21.669392249318417	27.529264002891324	28.59252183926882	22.20882190852144
48	22.18396055406587	24.79227019522056	31.231024276579483	21.79274497413409
49	20.523188404170423	25.55646221242484	31.491366992876074	22.428982390528667
50	19.745864204251458	31.52616166502608	27.670406906854662	21.0575672238678
51	17.749267347669488	32.20589242160816	25.986120293038482	24.058719937683865
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1077.0
1	1984.5
2	2892.0
3	14541.0
4	26190.0
5	17987.0
6	9784.0
7	9988.5
8	10193.0
9	11007.0
10	11821.0
11	12278.0
12	12735.0
13	12327.5
14	11920.0
15	11490.5
16	11061.0
17	10657.0
18	10253.0
19	9744.5
20	9236.0
21	8653.0
22	8070.0
23	8291.0
24	8512.0
25	10526.5
26	13417.0
27	14293.0
28	17856.0
29	21419.0
30	26473.0
31	31527.0
32	37562.5
33	43598.0
34	44852.5
35	46107.0
36	50196.0
37	54285.0
38	62052.0
39	69819.0
40	94630.0
41	119441.0
42	140092.0
43	160743.0
44	162050.5
45	163358.0
46	155620.5
47	147883.0
48	138302.0
49	128721.0
50	118966.0
51	109211.0
52	101829.5
53	94448.0
54	89856.0
55	85264.0
56	79307.0
57	73350.0
58	71518.0
59	69686.0
60	67185.5
61	64685.0
62	56572.5
63	48460.0
64	39220.0
65	29980.0
66	25513.0
67	21046.0
68	17512.5
69	13979.0
70	11848.0
71	9717.0
72	8098.0
73	6479.0
74	5050.5
75	2796.0
76	1970.0
77	1636.0
78	1302.0
79	982.5
80	663.0
81	491.0
82	319.0
83	219.5
84	120.0
85	87.5
86	55.0
87	43.0
88	31.0
89	19.5
90	8.0
91	5.0
92	2.0
93	2.0
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1781882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.70472270646843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.8552816664633	15.684897910440021
2	6.536234565724838	2.4451691019263446
3	1.9660743380943801	1.1032462594307653
4	0.9047470630359812	0.6769217173431896
5	0.5204821090641023	0.4867736761860947
6	0.34335978415802415	0.3853469730737217
7	0.23067011969680087	0.30202344379175794
8	0.17843537010615537	0.2670067295089363
9	0.15125134791802466	0.25462030696076093
>10	2.3096129773880625	11.476307678105629
>50	1.4466342834810255	19.948699119349282
>100	1.5517779009174604	45.08707186040588
>500	0.0042299241847432	0.5254103853655155
>1k	9.064123253021143E-4	0.36310226158690306
>5k	0.0	0.0
>10k+	3.021374417673714E-4	0.9934025765252267
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17578	0.9864850758916697	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3261	0.18300875142125012	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1821	0.10219531933090967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6836131685487592E-4	0.0	0.0	0.026488847185167145	0.0
2	1.6836131685487592E-4	0.0	0.0	0.11010830122308884	0.0
3	1.6836131685487592E-4	0.0	0.0	0.16173910505858413	0.0
4	1.6836131685487592E-4	0.0	0.0	0.34738551711056065	0.0
5	1.6836131685487592E-4	0.0	0.0	0.5573320792285908	0.0
6	1.6836131685487592E-4	0.0	0.0	0.8577447889366412	0.0
7	1.6836131685487592E-4	0.0	0.0	0.9853065466736854	0.0
8	1.6836131685487592E-4	0.0	0.0	1.2664138253823767	0.0
9	1.6836131685487592E-4	0.0	0.0	1.3493037137139272	0.0
10	1.6836131685487592E-4	0.0	0.0	1.5040838843425097	0.0
11	2.2448175580650121E-4	0.0	0.0	1.742034545497401	0.0
12	2.2448175580650121E-4	0.0	0.0	1.9152222201021167	0.0
13	2.2448175580650121E-4	0.0	0.0	1.990816451369956	0.0
14	2.2448175580650121E-4	0.0	0.0	2.020055200063753	0.0
15	2.2448175580650121E-4	0.0	0.0	2.057319171527632	0.0
16	2.2448175580650121E-4	0.0	0.0	2.149861775358862	0.0
17	2.2448175580650121E-4	0.0	0.0	2.247062375623077	0.0
18	2.2448175580650121E-4	0.0	0.0	2.382032031301736	0.0
19	2.2448175580650121E-4	0.0	0.0	2.4523509413081226	0.0
20	2.2448175580650121E-4	0.0	0.0	2.524634066677816	0.0
21	2.2448175580650121E-4	0.0	0.0	2.631375141563807	0.0
22	2.2448175580650121E-4	0.0	0.0	2.740641636202622	0.0
23	2.2448175580650121E-4	0.0	0.0	2.8670809851606336	0.0
24	2.2448175580650121E-4	0.0	0.0	2.953899304218798	0.0
25	2.2448175580650121E-4	0.0	0.0	3.0288762106581695	0.0
26	2.2448175580650121E-4	0.0	0.0	3.1028429491964116	0.0
27	2.2448175580650121E-4	0.0	0.0	3.1773147716852184	0.0
28	2.2448175580650121E-4	0.0	0.0	3.25161823285717	0.0
29	2.2448175580650121E-4	0.0	0.0	3.334508121188721	0.0
30	2.2448175580650121E-4	0.0	0.0	3.4402951486125346	0.0
31	2.2448175580650121E-4	0.0	0.0	3.542827190577154	0.0
32	2.2448175580650121E-4	0.0	0.0	3.6364360827484647	0.0
33	2.2448175580650121E-4	0.0	0.0	3.7229176791729195	0.0
34	2.2448175580650121E-4	0.0	0.0	3.817648980123263	0.0
35	2.2448175580650121E-4	0.0	0.0	3.947006591906759	0.0
36	2.2448175580650121E-4	0.0	0.0	4.060369878589042	0.0
37	2.2448175580650121E-4	0.0	0.0	4.175641260195681	0.0
38	2.2448175580650121E-4	0.0	0.0	4.279744674450946	0.0
39	2.2448175580650121E-4	0.0	0.0	4.391873311476293	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATA	30	2.1661763E-6	45.000004	25
CGGGTAC	45	3.8562575E-10	45.0	6
CGAAAGT	25	3.891673E-5	45.0	45
GATCACG	70	0.0	45.0	1
TACGCTA	20	7.0343324E-4	45.0	15
TACGCGA	20	7.0343324E-4	45.0	37
CGAATGT	20	7.0343324E-4	45.0	44
ACTCGTT	20	7.0343324E-4	45.0	33
CATACGT	20	7.0343324E-4	45.0	23
TTACGTC	45	3.8562575E-10	45.0	29
CGTAACA	25	3.891673E-5	45.0	40
ACGTAAG	95	0.0	45.0	1
GCGTACA	25	3.891673E-5	45.0	37
CGTTAGG	215	0.0	42.906975	2
CGTTTTT	9240	0.0	42.126625	1
GCGATAT	225	0.0	42.0	9
GTATGCG	70	0.0	41.785713	1
TTTGACG	130	0.0	41.538464	1
CGAAAGG	245	0.0	41.32653	2
CTAACGG	60	3.6379788E-12	41.250004	2
>>END_MODULE