FastQCFastQC Report
Thu 26 May 2016
SRR1547396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547396_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2488519
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT484331.9462579952172356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT216100.043.511111
AGGGCGA23300.039.8819736
TACGGGA11350.039.8458144
AAGGGAT53350.039.0112465
CGTTAGG4100.038.9634172
ATAGGGA45800.038.613544
GGGCGAT44850.038.5785947
ACGGGAT14700.038.2653055
AGGGATC35950.037.9902656
TAAGGGA44250.037.983054
TAGGGCG8250.037.9090925
GACCGAT16100.037.872679
TAATGCG1550.037.7419361
TAGGGAT52800.037.3295445
GGCGATT13350.037.2471928
AGGGATT61350.037.1882636
TATGGGA35250.037.0212754
AGGGATA33450.036.8609856
CGGGATA8200.036.7682956
GTTTTTT260800.036.5538752
Produced by FastQC (version 0.11.5)