##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547396_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2488519
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29692399374889	31.0	31.0	33.0	30.0	34.0
2	31.690875576999815	31.0	31.0	34.0	30.0	34.0
3	31.694111236442236	31.0	31.0	34.0	30.0	34.0
4	35.5198903444177	37.0	35.0	37.0	33.0	37.0
5	35.44379689285073	37.0	35.0	37.0	33.0	37.0
6	35.4839850529572	37.0	35.0	37.0	33.0	37.0
7	35.94094519672142	37.0	35.0	37.0	35.0	37.0
8	35.957114653333974	37.0	35.0	37.0	35.0	37.0
9	37.701439691639884	39.0	37.0	39.0	35.0	39.0
10	36.96538423054033	39.0	37.0	39.0	32.0	39.0
11	36.657501911779654	39.0	35.0	39.0	32.0	39.0
12	36.449108485810235	39.0	35.0	39.0	32.0	39.0
13	36.33458735898741	39.0	35.0	39.0	32.0	39.0
14	37.44402514105779	40.0	36.0	41.0	32.0	41.0
15	37.573673739280274	40.0	36.0	41.0	32.0	41.0
16	37.63990268910947	40.0	36.0	41.0	32.0	41.0
17	37.54580937497363	39.0	36.0	41.0	32.0	41.0
18	37.35245139779925	39.0	36.0	41.0	32.0	41.0
19	37.2399129763526	39.0	36.0	41.0	32.0	41.0
20	37.03207972291953	39.0	35.0	41.0	32.0	41.0
21	36.91686621641225	39.0	35.0	41.0	31.0	41.0
22	36.82110162711235	38.0	35.0	41.0	31.0	41.0
23	36.71675201193963	38.0	35.0	40.0	31.0	41.0
24	36.61038392714703	38.0	35.0	40.0	31.0	41.0
25	36.52649587967783	38.0	35.0	40.0	31.0	41.0
26	36.320794014431875	38.0	35.0	40.0	30.0	41.0
27	36.31221260516797	38.0	35.0	40.0	30.0	41.0
28	36.303942626116175	38.0	35.0	40.0	30.0	41.0
29	36.29972324904893	38.0	35.0	40.0	30.0	41.0
30	36.26761861171243	38.0	35.0	40.0	30.0	41.0
31	36.15358050310245	38.0	35.0	40.0	30.0	41.0
32	35.92072433443345	38.0	35.0	40.0	29.0	41.0
33	35.63567165852461	38.0	35.0	40.0	27.0	41.0
34	35.31365723950671	38.0	34.0	40.0	25.0	41.0
35	35.09527192679662	38.0	34.0	40.0	24.0	41.0
36	34.84176411753336	38.0	34.0	40.0	23.0	41.0
37	34.79414864825223	38.0	34.0	40.0	23.0	41.0
38	34.71100120191969	38.0	34.0	40.0	23.0	41.0
39	34.57566769632862	38.0	34.0	40.0	23.0	41.0
40	34.47154994597188	38.0	33.0	40.0	22.0	41.0
41	34.43664163303555	38.0	33.0	40.0	22.0	41.0
42	34.35056392979117	38.0	33.0	40.0	22.0	41.0
43	34.251478489816634	38.0	33.0	40.0	21.0	41.0
44	34.166742146634206	37.0	33.0	40.0	21.0	41.0
45	34.03007813080792	37.0	33.0	40.0	20.0	41.0
46	33.940131459715595	37.0	33.0	40.0	20.0	41.0
47	33.90603487455792	37.0	33.0	40.0	20.0	41.0
48	33.790052637733524	37.0	33.0	40.0	20.0	41.0
49	33.67527272245058	37.0	33.0	40.0	20.0	41.0
50	33.57951335714134	36.0	33.0	40.0	20.0	41.0
51	33.13276089111636	36.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	2.0
13	13.0
14	28.0
15	93.0
16	237.0
17	622.0
18	1287.0
19	2608.0
20	4462.0
21	6966.0
22	10539.0
23	15502.0
24	22756.0
25	34705.0
26	49835.0
27	57929.0
28	60112.0
29	62010.0
30	67812.0
31	78430.0
32	94497.0
33	117570.0
34	169608.0
35	224217.0
36	206763.0
37	252073.0
38	364015.0
39	583669.0
40	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.714895084184608	23.21682092843173	26.988140335677567	19.080143651706095
2	34.94484068636808	24.409900024874233	28.26420051444253	12.381058774315164
3	26.489892180851342	24.957856460006937	35.69564869707646	12.856602662065269
4	22.850096784473013	27.69904509469287	35.875996928293496	13.574861192540624
5	21.35539250453784	31.74719582209338	33.902292889867425	12.995118783501352
6	20.933615535987467	39.588727271119886	30.035535191814887	9.442122001077749
7	86.62586060222968	2.1769976439802146	10.28668055176593	0.9104612020241758
8	87.3445611626835	1.6831697889387223	10.011054767916178	0.9612142804615917
9	82.46672016568891	4.928674444519009	11.05175407541594	1.5528513143761409
10	34.09337039419832	39.882556653174035	17.608706222456007	8.415366730171641
11	24.349140995105927	25.080620240391976	34.70759114155849	15.862647622943607
12	25.478246298300316	23.380814050445263	33.72118115232393	17.419758498930488
13	21.48627356270939	26.23697066407771	34.898025693193425	17.37873008001948
14	17.671474479399194	29.29609940691632	34.72406680439249	18.308359309291994
15	17.25713165139587	26.913035423880626	39.149751317952564	16.680081606770937
16	21.06521991594197	25.74820606151691	37.376608336122814	15.809965686418307
17	20.53598947807913	25.1048917046645	33.667132941319714	20.691985875936652
18	20.693713811307045	25.679209200331606	35.464467018335	18.16260997002635
19	22.75236797468695	26.977089586215737	32.20666589244446	18.06387654665285
20	24.050610021462564	27.243352371430557	32.06839087826936	16.637646728837513
21	23.04961304293839	26.290858136907936	33.98941298017013	16.67011583998354
22	22.44632249140955	24.060937449141438	33.96285099691825	19.529889062530767
23	19.573690214943102	27.958717614774088	33.6854169086111	18.782175261671703
24	18.98579034357383	26.85380340676523	37.21241429139179	16.947991958269153
25	19.663542854203644	27.688315821579017	34.50425735146085	18.143883972756488
26	19.025492672549415	30.750820066071427	31.682418337975314	18.541268923403837
27	18.240969829846588	29.633569203208815	33.69494064542003	18.43052032152457
28	17.59106520786058	28.954088757208606	35.84175969723358	17.61308633769724
29	18.062671010347923	26.646290424143835	35.99104527632701	19.299993289181234
30	19.256433244029882	27.895065297873956	34.123669539995475	18.724831918100683
31	20.79208557378907	27.88333141117267	32.58488281584348	18.739700199194782
32	21.746990880921544	28.179973711271643	32.022218837790675	18.050816570016142
33	21.102591541394702	28.3831065786518	31.722442143298885	18.79185973665461
34	18.98526794450836	29.247877954719254	32.304836732209	19.46201736856339
35	19.437384243399386	28.966586150236346	32.38251345478977	19.213516151574492
36	21.67228781455958	29.46053455890833	30.364726972146887	18.5024506543852
37	19.827294868956194	31.133256366537687	30.647344866565213	18.392103897940903
38	19.87129694408602	30.792049407699924	30.042125456948494	19.294528191265567
39	19.954197657321483	30.38795363828848	30.167903078095847	19.489945626294194
40	21.609198081268417	29.36200205825232	29.827097964693056	19.201701895786208
41	19.158382957895842	28.769762256185306	30.96986601267662	21.10198877324224
42	20.280214858717173	29.607368880848405	29.541787705860393	20.570628554574025
43	20.25441638179174	29.666118683441837	29.88624961272146	20.19321532204496
44	19.801898237465736	30.589318385754737	30.1374030095812	19.47138036719832
45	19.616647491941993	30.682586711212572	29.00070282766577	20.700062969179662
46	20.031472534467287	31.05883459198021	29.27327458620971	19.636418287342792
47	19.6774467062538	30.229988197799575	30.38606496474409	19.706500131202535
48	20.164403004357208	29.079705640181974	31.363272693517715	19.392618661943107
49	20.04429140384301	28.842456095372388	31.12003565172699	19.993216849057614
50	19.178274306927133	30.031717660182622	30.742260758306443	20.0477472745838
51	18.41983123295422	30.903360593188157	30.069008916548356	20.607799257309267
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1130.0
1	1943.0
2	2756.0
3	35662.5
4	68569.0
5	44858.0
6	21147.0
7	21176.0
8	21205.0
9	22439.5
10	23674.0
11	25170.5
12	26667.0
13	26732.0
14	26797.0
15	26108.5
16	25420.0
17	24141.0
18	22862.0
19	22178.5
20	21495.0
21	21682.0
22	21869.0
23	22387.0
24	22905.0
25	25474.0
26	32133.0
27	36223.0
28	42950.0
29	49677.0
30	55707.0
31	61737.0
32	72035.5
33	82334.0
34	89775.0
35	97216.0
36	103885.0
37	110554.0
38	119295.0
39	128036.0
40	138624.5
41	149213.0
42	156902.0
43	164591.0
44	171681.0
45	178771.0
46	177690.0
47	176609.0
48	173419.5
49	170230.0
50	161262.0
51	152294.0
52	143818.0
53	135342.0
54	124156.0
55	112970.0
56	103656.5
57	94343.0
58	84805.5
59	75268.0
60	67474.5
61	59681.0
62	52334.0
63	44987.0
64	36487.0
65	27987.0
66	23430.0
67	18873.0
68	14532.0
69	10191.0
70	8089.5
71	5988.0
72	5189.5
73	4391.0
74	3714.0
75	2577.5
76	2118.0
77	1465.5
78	813.0
79	551.5
80	290.0
81	213.5
82	137.0
83	87.0
84	37.0
85	30.0
86	23.0
87	18.0
88	13.0
89	9.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2488519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.974871873248528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.24037159348494	18.518280123959062
2	6.527385797105824	3.129864763057486
3	2.8023303827764465	2.015565356207304
4	1.7171215898613947	1.6467108043086296
5	1.1237862726444003	1.3471315949782519
6	0.8661697901298209	1.2459785843304614
7	0.6620374343803397	1.1110583865193975
8	0.5544080818084055	1.0633490181480008
9	0.4879965330089203	1.0529688918130526
>10	6.33254724748675	36.08895248197583
>50	1.4810563770958531	24.392736853873707
>100	0.20308809411399364	6.135107175980066
>500	0.001190564272014377	0.1829238652722238
>1k	3.401612205755363E-4	0.09443862504914631
>5k	0.0	0.0
>10k+	1.7008061028776815E-4	1.974933474527333
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48433	1.9462579952172356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0184543497558184E-5	0.0	0.0	0.020654855357744906	0.0
2	4.0184543497558184E-5	0.0	0.0	0.05320433559076704	0.0
3	4.0184543497558184E-5	0.0	0.0	0.08720045938970127	0.0
4	4.0184543497558184E-5	0.0	0.0	0.1387572286970684	0.0
5	4.0184543497558184E-5	0.0	0.0	0.25260004042565076	0.0
6	4.0184543497558184E-5	0.0	0.0	0.49869018480469707	0.0
7	4.0184543497558184E-5	0.0	0.0	0.6497438838120183	0.0
8	4.0184543497558184E-5	0.0	0.0	0.9221950887254629	0.0
9	4.0184543497558184E-5	0.0	4.0184543497558184E-5	1.0597065965741068	0.0
10	4.0184543497558184E-5	0.0	4.0184543497558184E-5	1.2177925906935008	0.0
11	4.0184543497558184E-5	0.0	4.0184543497558184E-5	1.3925149858208838	0.0
12	4.0184543497558184E-5	0.0	4.0184543497558184E-5	1.5334421798668203	0.0
13	4.0184543497558184E-5	0.0	4.0184543497558184E-5	1.622250020996424	0.0
14	4.0184543497558184E-5	0.0	4.0184543497558184E-5	1.68337071165621	0.0
15	4.0184543497558184E-5	0.0	4.0184543497558184E-5	1.7367357854209673	0.0
16	4.0184543497558184E-5	0.0	4.0184543497558184E-5	1.8072194747156842	0.0
17	4.0184543497558184E-5	0.0	4.0184543497558184E-5	1.8937769814094247	0.0
18	4.0184543497558184E-5	0.0	4.0184543497558184E-5	1.9936757565443544	0.0
19	4.0184543497558184E-5	0.0	4.0184543497558184E-5	2.0699862046462174	0.0
20	4.0184543497558184E-5	0.0	4.0184543497558184E-5	2.1476629272269974	0.0
21	4.0184543497558184E-5	0.0	4.0184543497558184E-5	2.244748784317098	0.0
22	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	2.361605436807997	0.0
23	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	2.4815161146047107	0.0
24	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	2.5788430789557966	0.0
25	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	2.6690171945643173	0.0
26	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	2.758709095650867	0.0
27	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	2.8498074557598314	0.0
28	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	2.951152874460673	0.0
29	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	3.063227566275363	0.0
30	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	3.1996942759930707	0.0
31	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	3.3220562109431353	0.0
32	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	3.4389932325210295	0.0
33	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	3.5617971974495672	0.0
34	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	3.6841993169431295	0.0
35	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	3.821590271161281	0.0
36	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	3.947568815026126	0.0
37	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	4.076078985131317	0.0
38	4.0184543497558184E-5	4.0184543497558184E-5	4.0184543497558184E-5	4.221667586222971	0.0
39	8.036908699511637E-5	4.0184543497558184E-5	4.0184543497558184E-5	4.433922344977073	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	21610	0.0	43.51111	1
AGGGCGA	2330	0.0	39.881973	6
TACGGGA	1135	0.0	39.845814	4
AAGGGAT	5335	0.0	39.011246	5
CGTTAGG	410	0.0	38.963417	2
ATAGGGA	4580	0.0	38.61354	4
GGGCGAT	4485	0.0	38.578594	7
ACGGGAT	1470	0.0	38.265305	5
AGGGATC	3595	0.0	37.990265	6
TAAGGGA	4425	0.0	37.98305	4
TAGGGCG	825	0.0	37.909092	5
GACCGAT	1610	0.0	37.87267	9
TAATGCG	155	0.0	37.741936	1
TAGGGAT	5280	0.0	37.329544	5
GGCGATT	1335	0.0	37.247192	8
AGGGATT	6135	0.0	37.188263	6
TATGGGA	3525	0.0	37.021275	4
AGGGATA	3345	0.0	36.860985	6
CGGGATA	820	0.0	36.768295	6
GTTTTTT	26080	0.0	36.553875	2
>>END_MODULE