FastQCFastQC Report
Thu 26 May 2016
SRR1547395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547395_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2416327
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT503162.0823340549519993No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC253.8920873E-545.038
GCCGATC502.1827873E-1145.09
TCGCGAT253.8920873E-545.037
CGTTTTT238400.043.9807051
GGCGATA7800.039.5192348
AGGGCGA20850.039.4964036
ACGGGTA3450.039.1304365
GTTTTTT272250.038.9008262
GGGCGAT40850.038.886177
GCGATAT2150.038.7209329
AACGGGT5350.038.691594
TAGCGCG356.251148E-638.571431
GTTCGCA356.251148E-638.5714310
TAGGGCG9500.038.368425
TAATCGT2700.038.33333221
CGTTAGG5050.038.3168332
GACCGAT12700.038.2677159
TCGTTAG1000.038.251
GCGATAC1000.038.259
TCGTCCC8600.038.19767438
Produced by FastQC (version 0.11.5)