FastQCFastQC Report
Thu 26 May 2016
SRR1547394_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547394_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1580476
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155580.9843869821496815No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35370.22379333820950145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG207.0340896E-445.00000420
GGTACGC207.0340896E-445.0000048
CGAACGC207.0340896E-445.00000433
CTCGTTA207.0340896E-445.00000434
GCACGAT253.8914717E-545.00000415
TTCGTTA253.8914717E-545.00000413
ACCTATC302.1660198E-645.00000430
CCCGTAG253.8914717E-545.0000041
CCGTAGT253.8914717E-545.00000417
TACGCCG207.0340896E-445.0000041
CGTAGTC253.8914717E-545.00000418
CGTAGGT253.8914717E-545.00000421
TATTACG302.1660198E-645.0000041
ACGTCGA302.1660198E-645.00000435
ACGTCCA207.0340896E-445.00000411
ATGCGTC551.8189894E-1245.00000412
AACCTCG253.8914717E-545.00000435
GTATAAC253.8914717E-545.00000439
CGACTTA207.0340896E-445.00000434
CTTACGA253.8914717E-545.00000434