##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547394_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1580476
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.426172241780325	31.0	31.0	33.0	30.0	34.0
2	31.828096725290354	31.0	31.0	34.0	30.0	34.0
3	31.87784313080363	31.0	31.0	34.0	30.0	34.0
4	35.6402374980702	37.0	35.0	37.0	33.0	37.0
5	35.56015909131173	37.0	35.0	37.0	33.0	37.0
6	35.59723146697577	37.0	35.0	37.0	33.0	37.0
7	35.97579653218398	37.0	35.0	37.0	35.0	37.0
8	35.98195037444415	37.0	35.0	37.0	35.0	37.0
9	37.75191840939059	39.0	38.0	39.0	35.0	39.0
10	37.12760143146748	39.0	37.0	39.0	33.0	39.0
11	36.75544013322568	39.0	35.0	39.0	32.0	39.0
12	35.81036409284292	37.0	35.0	39.0	31.0	39.0
13	35.247179963504664	37.0	35.0	39.0	30.0	39.0
14	36.29075544329683	38.0	35.0	41.0	31.0	41.0
15	36.68127640027435	38.0	35.0	41.0	31.0	41.0
16	36.91219670529638	38.0	35.0	41.0	32.0	41.0
17	36.86300772678611	38.0	35.0	41.0	32.0	41.0
18	36.7537646886128	38.0	35.0	40.0	32.0	41.0
19	36.63787555141616	37.0	35.0	40.0	32.0	41.0
20	36.40759176349403	37.0	35.0	40.0	31.0	41.0
21	36.21968508221574	37.0	35.0	40.0	31.0	41.0
22	36.085729236002315	37.0	35.0	40.0	31.0	41.0
23	36.07290018956314	36.0	35.0	40.0	31.0	41.0
24	35.962516988552814	36.0	35.0	40.0	31.0	41.0
25	35.85020841822337	36.0	34.0	40.0	31.0	41.0
26	35.63246072702148	36.0	34.0	40.0	30.0	41.0
27	35.58670362599622	36.0	34.0	40.0	30.0	41.0
28	35.66578739569598	36.0	34.0	40.0	30.0	41.0
29	35.753026936188846	36.0	35.0	40.0	30.0	41.0
30	35.781780299099765	36.0	35.0	40.0	30.0	41.0
31	35.59852917728583	36.0	34.0	40.0	30.0	41.0
32	35.33687888965097	36.0	34.0	40.0	29.0	41.0
33	35.07400808364063	36.0	34.0	40.0	28.0	41.0
34	34.86400805833179	36.0	34.0	40.0	27.0	41.0
35	34.69518929740154	36.0	34.0	40.0	26.0	41.0
36	34.3883526228807	35.0	33.0	40.0	24.0	41.0
37	34.300865055843936	35.0	33.0	40.0	23.0	41.0
38	34.27022049053576	35.0	33.0	40.0	24.0	41.0
39	34.206024640677875	35.0	33.0	40.0	23.0	41.0
40	34.027334170212015	35.0	33.0	40.0	23.0	41.0
41	34.04179500353058	35.0	33.0	40.0	23.0	41.0
42	33.959599513058095	35.0	33.0	40.0	23.0	41.0
43	33.838380336050655	35.0	33.0	40.0	23.0	41.0
44	33.74406950817349	35.0	33.0	40.0	23.0	41.0
45	33.59226713977308	35.0	33.0	40.0	23.0	41.0
46	33.53452061277741	35.0	33.0	40.0	23.0	41.0
47	33.52261280778702	35.0	33.0	39.0	23.0	41.0
48	33.47340168404961	35.0	33.0	39.0	23.0	41.0
49	33.375153434788004	35.0	33.0	39.0	22.0	41.0
50	33.204699090653705	35.0	33.0	39.0	22.0	41.0
51	32.68563331553279	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	1.0
13	3.0
14	19.0
15	67.0
16	130.0
17	395.0
18	858.0
19	1662.0
20	2851.0
21	4650.0
22	6888.0
23	10346.0
24	14798.0
25	21387.0
26	29830.0
27	35701.0
28	37678.0
29	40235.0
30	45708.0
31	54501.0
32	67253.0
33	89106.0
34	157441.0
35	244061.0
36	107130.0
37	127612.0
38	182493.0
39	297558.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.686704511805303	23.965058627907034	29.121036953424156	16.2271999068635
2	31.740880595466177	26.239942903277242	28.484962758055165	13.534213743201414
3	27.944366127672932	25.564956380229752	33.03466803671805	13.456009455379265
4	24.599551021337877	27.749867761357972	33.12628600497571	14.52429521232844
5	22.757827388710744	32.04591528121908	31.63262207081917	13.563635259251011
6	21.87923132018455	39.963593246591536	28.322922967511055	9.834252465712861
7	87.1184377364794	3.5587380004504974	8.17968763840767	1.1431366246624435
8	88.22835652044067	2.4932362149124696	7.726722835398955	1.5516844292478975
9	83.58709654559765	5.095933123944938	9.090362650239548	2.2266076802178585
10	50.58950594630984	27.264760742966043	13.525608740657876	8.620124570066233
11	46.38450694600867	19.524307866743943	22.12466370890795	11.966521478339438
12	43.32479582100582	19.014398193961817	23.98277480961432	13.678031175418038
13	21.366854036378914	39.3152442681825	24.414480194574292	14.903421500864297
14	15.52038752882043	40.73620858526166	27.23135308603231	16.512050799885607
15	13.933523824468072	23.826429506047546	48.212247449502556	14.02779921998183
16	15.991258329769007	18.564976627294563	48.50684224246366	16.93692280047277
17	16.482186379293324	19.387766723442812	29.614242797739415	34.51580409952445
18	20.059336554303894	23.65230474869596	35.48842247525429	20.799936221745856
19	28.425866637645875	24.15595048580301	27.49273003829226	19.925452838258852
20	32.4339629326861	21.84493785416545	27.30379961479959	18.41729959834885
21	23.128791579245746	27.930762631004836	29.10990106777958	19.830544721969837
22	23.082792778884336	24.67857784616786	26.594076721190323	25.644552653757476
23	20.889213123135058	30.098274190813402	25.259099157469016	23.753413528582527
24	20.640996763000512	22.748273305004314	39.44950761669269	17.161222315302478
25	17.767748450466822	24.410367509535103	36.500016450740155	21.32186758925792
26	17.437151845393416	35.40616877447047	27.73866860363587	19.418010776500243
27	18.368706642808874	35.36124559942701	28.325517122689618	17.944530635074496
28	15.68900761542725	30.301314287594373	37.97014317205702	16.03953492492135
29	15.799417390710142	25.229804185574473	37.725343504108885	21.2454349196065
30	18.250894034455442	31.20667444491406	31.36668952897735	19.175741991653148
31	27.63041007898886	27.833386903692304	26.290623837375577	18.24557917994326
32	29.561916789625403	26.86728555194764	27.18908733824493	16.381710320182023
33	27.985366433909785	28.483380956117017	25.25859298084881	18.27265962912439
34	20.439158835692538	27.691847266266617	27.58289274876683	24.286101149274018
35	20.26921003545767	25.33584818750807	31.44558980965228	22.94935196738198
36	30.051263037211573	25.015944563536557	25.926935935756063	19.005856463495807
37	20.992473153657507	32.553040982590055	28.941597341560392	17.51288852219205
38	20.5869624087933	31.8734988699607	25.657523429650308	21.882015291595696
39	21.118321315856743	31.853378349307427	27.440340758100724	19.58795957673511
40	25.357487238021964	26.403564495759507	26.75725540912991	21.481692857088625
41	16.696299089641347	25.359638488657847	29.350904411076158	28.59315801062465
42	21.741614551565476	26.46449550641705	24.74811385936895	27.04577608264852
43	22.652099747164776	26.33523065203141	26.436339431917975	24.57633016888583
44	19.358408479470743	30.156927406680012	29.13438736178215	21.3502767520671
45	18.023430915749433	36.349555450383306	23.403708756096265	22.223304877771
46	21.47403693570798	33.21796724531091	25.757430040063877	19.550565778917235
47	21.168242985024765	28.04237457576072	27.122588384765095	23.66679405444942
48	23.69109053221941	23.813205641844608	31.11081724746216	21.38488657847383
49	20.52236161763924	25.03695089327519	32.01307707298307	22.427610416102493
50	19.730005390781006	32.59106750118318	27.22945492370653	20.449472184329277
51	18.63641080282143	34.00931111892873	23.86331712724521	23.490960951004634
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	863.0
1	1548.0
2	2233.0
3	12851.5
4	23470.0
5	16335.0
6	9200.0
7	9513.5
8	9827.0
9	10733.0
10	11639.0
11	11997.0
12	12355.0
13	11920.5
14	11486.0
15	10879.5
16	10273.0
17	9823.5
18	9374.0
19	9531.5
20	9689.0
21	9126.0
22	8563.0
23	8266.5
24	7970.0
25	8880.5
26	10795.5
27	11800.0
28	14911.0
29	18022.0
30	19902.5
31	21783.0
32	24980.0
33	28177.0
34	33745.0
35	39313.0
36	41413.0
37	43513.0
38	51815.0
39	60117.0
40	82031.5
41	103946.0
42	125447.0
43	146948.0
44	150345.0
45	153742.0
46	144774.5
47	135807.0
48	127783.5
49	119760.0
50	109855.0
51	99950.0
52	92651.5
53	85353.0
54	80549.5
55	75746.0
56	71280.0
57	66814.0
58	62683.5
59	58553.0
60	57483.5
61	56414.0
62	48422.0
63	40430.0
64	33726.5
65	27023.0
66	21314.5
67	15606.0
68	13874.5
69	12143.0
70	10538.5
71	8934.0
72	7327.0
73	5720.0
74	4634.5
75	2978.5
76	2408.0
77	1813.0
78	1218.0
79	882.5
80	547.0
81	398.5
82	250.0
83	170.5
84	91.0
85	63.0
86	35.0
87	23.5
88	12.0
89	9.0
90	6.0
91	6.0
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	2.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1580476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.3090966006786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.7578480763802	15.335305314954073
2	6.646630433456941	2.433875973503468
3	1.8994427711700992	1.0433124355444199
4	0.8878865108594102	0.6502559959105764
5	0.478850784580543	0.438366263609795
6	0.3337803383773334	0.3666729875254673
7	0.25152085963365833	0.32235838012828605
8	0.18995859029302056	0.27823761438429134
9	0.1596843809242462	0.2631309080365425
>10	2.3176285067477767	11.242799114904061
>50	1.4720732063669502	19.925413723667166
>100	1.599126098984474	45.819280322797425
>500	0.003828991530002005	0.45224340610843883
>1k	0.0013923605563643654	0.43720261639006347
>5k	0.0	0.0
>10k+	3.4809013909109136E-4	0.9915449425359485
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15558	0.9843869821496815	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3537	0.22379333820950145	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.327207752601115E-5	0.0	0.0	0.022398315444207947	0.0
2	6.327207752601115E-5	0.0	0.0	0.1061705460886467	0.0
3	6.327207752601115E-5	0.0	0.0	0.1650768502653631	0.0
4	6.327207752601115E-5	0.0	0.0	0.34603499198975496	0.0
5	6.327207752601115E-5	0.0	0.0	0.5594517094849906	0.0
6	6.327207752601115E-5	0.0	0.0	0.8353812395759252	0.0
7	6.327207752601115E-5	0.0	0.0	0.9479422654946991	0.0
8	6.327207752601115E-5	0.0	0.0	1.1918561243574721	0.0
9	6.327207752601115E-5	0.0	0.0	1.2637332044270206	0.0
10	6.327207752601115E-5	0.0	0.0	1.3999579873405228	0.0
11	6.327207752601115E-5	0.0	0.0	1.634381034574394	0.0
12	1.265441550520223E-4	0.0	0.0	1.8062279971350403	0.0
13	1.265441550520223E-4	0.0	0.0	1.8788643421349012	0.0
14	1.265441550520223E-4	0.0	0.0	1.9084756744170743	0.0
15	1.265441550520223E-4	0.0	0.0	1.9506148780493977	0.0
16	1.265441550520223E-4	0.0	0.0	2.053938180649374	0.0
17	1.265441550520223E-4	0.0	0.0	2.156249130008934	0.0
18	1.265441550520223E-4	0.0	0.0	2.293106633697696	0.0
19	1.265441550520223E-4	0.0	0.0	2.3581503293944355	0.0
20	1.265441550520223E-4	0.0	0.0	2.429078328301094	0.0
21	1.265441550520223E-4	0.0	0.0	2.536261227630157	0.0
22	1.265441550520223E-4	0.0	0.0	2.6460382821377864	0.0
23	1.265441550520223E-4	0.0	0.0	2.7662552294372076	0.0
24	1.265441550520223E-4	0.0	0.0	2.8506601808569063	0.0
25	1.265441550520223E-4	0.0	0.0	2.917791855112004	0.0
26	1.265441550520223E-4	0.0	0.0	2.9853031618322583	0.0
27	1.265441550520223E-4	0.0	0.0	3.0473730698852752	0.0
28	1.265441550520223E-4	0.0	0.0	3.1179214363267773	0.0
29	1.265441550520223E-4	0.0	0.0	3.18941888393117	0.0
30	1.265441550520223E-4	0.0	0.0	3.285782258003285	0.0
31	1.265441550520223E-4	0.0	0.0	3.386321589192117	0.0
32	1.265441550520223E-4	0.0	0.0	3.471549077619654	0.0
33	1.265441550520223E-4	0.0	0.0	3.552284248542844	0.0
34	1.265441550520223E-4	0.0	0.0	3.6526970355766237	0.0
35	1.265441550520223E-4	0.0	0.0	3.7829109711251547	0.0
36	1.265441550520223E-4	0.0	0.0	3.887879347740807	0.0
37	1.265441550520223E-4	0.0	0.0	3.989684120480159	0.0
38	1.265441550520223E-4	0.0	0.0	4.091172532831881	0.0
39	1.265441550520223E-4	0.0	0.0	4.19126895947803	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	7.0340896E-4	45.000004	20
GGTACGC	20	7.0340896E-4	45.000004	8
CGAACGC	20	7.0340896E-4	45.000004	33
CTCGTTA	20	7.0340896E-4	45.000004	34
GCACGAT	25	3.8914717E-5	45.000004	15
TTCGTTA	25	3.8914717E-5	45.000004	13
ACCTATC	30	2.1660198E-6	45.000004	30
CCCGTAG	25	3.8914717E-5	45.000004	1
CCGTAGT	25	3.8914717E-5	45.000004	17
TACGCCG	20	7.0340896E-4	45.000004	1
CGTAGTC	25	3.8914717E-5	45.000004	18
CGTAGGT	25	3.8914717E-5	45.000004	21
TATTACG	30	2.1660198E-6	45.000004	1
ACGTCGA	30	2.1660198E-6	45.000004	35
ACGTCCA	20	7.0340896E-4	45.000004	11
ATGCGTC	55	1.8189894E-12	45.000004	12
AACCTCG	25	3.8914717E-5	45.000004	35
GTATAAC	25	3.8914717E-5	45.000004	39
CGACTTA	20	7.0340896E-4	45.000004	34
CTTACGA	25	3.8914717E-5	45.000004	34
>>END_MODULE