FastQCFastQC Report
Thu 26 May 2016
SRR1547393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547393_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4969078
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT506941.020189258449958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT260850.042.4295581
TAGGGAC56700.039.6428575
TACGGGA19600.039.604594
TAGGGAT88100.038.7429055
TACGGGT5950.038.5714264
AGGGCGA47550.038.517356
ACGGGAT24050.038.0769235
AAGGGAT95750.037.9268955
ATAGGGA77450.037.8534554
TCGTAAG2450.037.653061
GGGCGAT91750.037.569487
TCGATAG2400.037.5000041
CGCGTAA301.1404679E-437.50000431
TAAGGGA70750.037.4946984
AGGGATT107600.037.3048366
GGCGATA20700.037.2826088
GTAGGGA70200.037.0833324
ACGGGTA5100.037.0588235
AGGGATC67800.036.969036
GCGATAA6150.036.9512189
Produced by FastQC (version 0.11.5)