##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547393_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4969078
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.336557204374735	31.0	31.0	33.0	30.0	34.0
2	31.75047886147088	31.0	31.0	34.0	30.0	34.0
3	31.82435594691812	31.0	31.0	34.0	30.0	34.0
4	35.617810587799184	37.0	35.0	37.0	33.0	37.0
5	35.52751959216579	37.0	35.0	37.0	33.0	37.0
6	35.562871422022354	37.0	35.0	37.0	33.0	37.0
7	35.961888905748715	37.0	35.0	37.0	35.0	37.0
8	35.96904174174767	37.0	35.0	37.0	35.0	37.0
9	37.72300696426983	39.0	37.0	39.0	35.0	39.0
10	36.98421135671447	39.0	37.0	39.0	32.0	39.0
11	36.70129126570362	39.0	35.0	39.0	32.0	39.0
12	36.41923632512913	39.0	35.0	39.0	32.0	39.0
13	36.29051264641046	39.0	35.0	39.0	32.0	39.0
14	37.36396812446897	39.0	36.0	41.0	32.0	41.0
15	37.503490385942825	39.0	36.0	41.0	32.0	41.0
16	37.57149817330298	39.0	36.0	41.0	32.0	41.0
17	37.479723803892796	39.0	36.0	41.0	32.0	41.0
18	37.330593925070204	39.0	36.0	41.0	32.0	41.0
19	37.26501395228652	39.0	36.0	41.0	32.0	41.0
20	37.12045252660554	39.0	35.0	41.0	32.0	41.0
21	37.00333764130891	39.0	35.0	41.0	31.0	41.0
22	36.91443000089755	39.0	35.0	41.0	31.0	41.0
23	36.826852788384485	38.0	35.0	41.0	31.0	41.0
24	36.719397441537446	38.0	35.0	40.0	31.0	41.0
25	36.62914327366163	38.0	35.0	40.0	31.0	41.0
26	36.42130210071164	38.0	35.0	40.0	30.0	41.0
27	36.43669026728902	38.0	35.0	40.0	30.0	41.0
28	36.48837792443588	38.0	35.0	40.0	30.0	41.0
29	36.49594190310557	38.0	35.0	40.0	30.0	41.0
30	36.4752573817517	38.0	35.0	40.0	30.0	41.0
31	36.403508658950415	38.0	35.0	40.0	30.0	41.0
32	36.24145243846041	38.0	35.0	40.0	30.0	41.0
33	36.039965361783416	38.0	35.0	40.0	30.0	41.0
34	35.80930627371919	38.0	35.0	40.0	28.0	41.0
35	35.6541420762564	38.0	35.0	40.0	27.0	41.0
36	35.437873585401555	38.0	34.0	40.0	26.0	41.0
37	35.40756273095331	38.0	34.0	40.0	26.0	41.0
38	35.33675905268543	38.0	34.0	40.0	26.0	41.0
39	35.20579713178179	38.0	34.0	40.0	25.0	41.0
40	35.09974405714702	38.0	34.0	40.0	25.0	41.0
41	35.073748892651714	38.0	34.0	40.0	25.0	41.0
42	35.003520975118526	38.0	34.0	40.0	24.0	41.0
43	34.89848780799979	38.0	34.0	40.0	24.0	41.0
44	34.814685541261376	38.0	34.0	40.0	24.0	41.0
45	34.660583110186636	38.0	33.0	40.0	23.0	41.0
46	34.590554022295485	37.0	33.0	40.0	23.0	41.0
47	34.55317344585857	37.0	33.0	40.0	23.0	41.0
48	34.436788072153426	37.0	33.0	40.0	23.0	41.0
49	34.32499228227047	37.0	33.0	40.0	24.0	41.0
50	34.21389400609127	37.0	33.0	40.0	23.0	41.0
51	33.75210793632139	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	6.0
13	16.0
14	55.0
15	151.0
16	369.0
17	865.0
18	2121.0
19	3936.0
20	7002.0
21	11134.0
22	17123.0
23	25315.0
24	36865.0
25	54150.0
26	77046.0
27	93552.0
28	102695.0
29	112117.0
30	129796.0
31	155214.0
32	190557.0
33	239943.0
34	357993.0
35	461033.0
36	403789.0
37	511580.0
38	753783.0
39	1220529.0
40	339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.251909911657656	24.786831681853254	29.013269664915704	15.947988741573385
2	30.901145041393995	25.993152049535144	30.16531437019101	12.94038853887985
3	27.659054657624615	25.58124464940981	33.65622354891592	13.103477144049661
4	24.208313896461277	28.563125795167633	33.01236164938445	14.216198658986636
5	22.54365900474897	32.17860536703187	31.644502259775354	13.633233368443804
6	21.544701049168477	40.684328159066936	27.776460743824106	9.994510047940484
7	89.68510858553638	2.256696312676114	7.078959114749256	0.9792359870382393
8	90.54104202027015	1.5919049771406284	6.880250219457211	0.9868027831320016
9	85.52751637225255	4.40622988812009	8.25915793634151	1.8070958032858409
10	37.596793610404184	35.914127329053805	16.152473356224235	10.336605704317783
11	27.879377220482347	24.20950123946535	30.132692624265506	17.778428915786794
12	27.924214512229433	22.811092923073456	30.549027405084	18.715665159613113
13	23.36894289041146	26.121304596144395	31.516490584369976	18.993261929074166
14	19.612089003231585	28.48965140011889	31.91767164854325	19.98058794810627
15	19.245361010634166	25.811166578588622	36.77738606638898	18.166086344388237
16	23.228494300149848	24.440771507309808	34.671140199449475	17.659593993090873
17	22.633897073058623	24.257578568901515	30.95924435076286	22.149280007277003
18	22.650781493065715	24.916372816043538	33.135301961450395	19.29754372944035
19	25.141404502002185	25.797481947355223	29.75708974582407	19.30402380481852
20	26.270728694538505	26.487227610433965	29.54753376783379	17.694509927193735
21	25.030297371061593	25.26557240598759	31.841540825078617	17.8625893978722
22	24.727444407191836	23.026927731864948	31.477811376677927	20.76781648426529
23	22.07204233863908	26.25122809503091	31.334162997642622	20.34256656868739
24	21.4098470581464	25.203649449656456	34.27092510924562	19.115578382951526
25	21.550879257681206	26.82511725515277	31.67720450353164	19.94679898363439
26	20.52767133057682	29.070000511161226	30.051953299988448	20.350374858273508
27	19.751974108677707	28.48598874881819	31.742226626347986	20.019810516156117
28	18.981207378914156	27.669539500084323	33.510361479534026	19.83889164146749
29	20.05138579028142	25.87427687792383	33.489613163649274	20.58472416814548
30	21.27700551289394	26.88484664559502	32.102333672363365	19.735814169147677
31	23.37113645630034	26.771425202019365	30.430896838407445	19.42654150327284
32	24.114010687696993	26.68380331321022	29.97137497137296	19.23081102771983
33	23.51858835784023	26.759390776317055	30.046821563275923	19.675199302566796
34	20.79108035736207	27.216578206258784	31.168659457549268	20.823681978829875
35	21.00981308806181	27.527561451037798	31.31256945453462	20.15005600636577
36	23.571173565800336	27.670304229476777	29.289699215830385	19.468822988892505
37	21.74151824543708	29.231257790680687	29.589875626826544	19.43734833705569
38	21.834593862281896	28.997129849843372	28.912144264992417	20.256132022882316
39	21.626386222957255	28.755716855320042	29.083101533121436	20.534795388601264
40	23.055504461793515	27.158036158820607	28.765919955372006	21.02053942401387
41	20.00206074446809	26.91356424672746	29.872060772642328	23.212314236162122
42	21.135872691070656	27.176369539781824	28.7916993856808	22.89605838346671
43	21.433312175820145	27.360729696736495	28.829613863980402	22.37634426346296
44	20.935694710366793	27.958104098989793	29.571844112730773	21.534357077912645
45	20.43745338672486	28.887008817329896	28.573811882204303	22.10172591374094
46	21.387347914442074	28.9974920900819	28.878938909793728	20.736221085682295
47	20.959260450329015	27.679259613151576	29.927765271545343	21.433714664974065
48	21.182621806298872	27.190054171015227	30.967777120826035	20.659546901859862
49	21.691508968062085	26.398197009586085	30.42578522615262	21.484508796199215
50	20.694261591385764	28.315252849723834	30.093671300792625	20.89681425809778
51	20.230191596911943	28.67529952236612	29.288833864149446	21.80567501657249
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1922.0
1	3665.0
2	5408.0
3	43307.5
4	81207.0
5	54867.5
6	28528.0
7	27838.0
8	27148.0
9	27879.5
10	28611.0
11	28763.0
12	28915.0
13	28664.5
14	28414.0
15	27373.0
16	26332.0
17	25634.0
18	24936.0
19	24192.5
20	23449.0
21	25472.0
22	27495.0
23	29635.0
24	31775.0
25	37966.5
26	53563.0
27	62968.0
28	72990.5
29	83013.0
30	98212.0
31	113411.0
32	129453.0
33	145495.0
34	165597.5
35	185700.0
36	194714.0
37	203728.0
38	225096.5
39	246465.0
40	268168.5
41	289872.0
42	311341.5
43	332811.0
44	345101.5
45	357392.0
46	357987.0
47	358582.0
48	358627.0
49	358672.0
50	344992.0
51	331312.0
52	315086.5
53	298861.0
54	274642.5
55	250424.0
56	238740.5
57	227057.0
58	211206.5
59	195356.0
60	176792.5
61	158229.0
62	140732.0
63	123235.0
64	105616.5
65	87998.0
66	72199.5
67	56401.0
68	46047.5
69	35694.0
70	29795.0
71	23896.0
72	19131.0
73	14366.0
74	12261.0
75	7686.0
76	5216.0
77	3800.5
78	2385.0
79	1746.0
80	1107.0
81	792.5
82	478.0
83	329.0
84	180.0
85	179.0
86	178.0
87	137.0
88	96.0
89	58.5
90	21.0
91	17.5
92	14.0
93	9.0
94	4.0
95	3.5
96	3.0
97	3.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4969078.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.046512610490137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.71738356775042	15.373846640089276
2	6.491308117870835	2.472735638512077
3	2.1029218070261804	1.2015998014919658
4	1.1324423442850688	0.8627630956431431
5	0.7229998806970486	0.6885313172539601
6	0.5211726080443874	0.5955912390815675
7	0.4063170594394819	0.5417246097529953
8	0.3342616395152088	0.5093214825783626
9	0.27299732486341877	0.46796822915770603
>10	4.224381040261676	20.582253419315748
>50	1.9945036394830247	27.439542875270767
>100	1.077173431901198	27.88215073420739
>500	0.001710031089699006	0.2343827887583915
>1k	3.2063082930999185E-4	0.11564431326305698
>5k	0.0	0.0
>10k+	1.0687694310333062E-4	1.031943815623554
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50694	1.020189258449958	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0124457696176233E-5	0.0	0.0	0.023364495385260607	0.0
2	2.0124457696176233E-5	0.0	0.0	0.06810116484386038	0.0
3	2.0124457696176233E-5	0.0	0.0	0.10235299184275232	0.0
4	2.0124457696176233E-5	0.0	0.0	0.17152075294451002	0.0
5	2.0124457696176233E-5	0.0	0.0	0.3040201824161343	0.0
6	2.0124457696176233E-5	0.0	0.0	0.5263551910434894	0.0
7	2.0124457696176233E-5	0.0	0.0	0.6431977924274885	0.0
8	2.0124457696176233E-5	0.0	0.0	0.8501778398326612	0.0
9	2.0124457696176233E-5	0.0	0.0	0.9526113295061981	0.0
10	2.0124457696176233E-5	0.0	0.0	1.1112926784405477	0.0
11	2.0124457696176233E-5	0.0	0.0	1.2952100973259024	0.0
12	2.0124457696176233E-5	0.0	0.0	1.436544163726148	0.0
13	2.0124457696176233E-5	0.0	0.0	1.5122724980368591	0.0
14	2.0124457696176233E-5	0.0	0.0	1.5599674627767968	0.0
15	2.0124457696176233E-5	0.0	0.0	1.6045028876584349	0.0
16	2.0124457696176233E-5	0.0	0.0	1.6866710484319223	0.0
17	2.0124457696176233E-5	0.0	0.0	1.7880781907629544	0.0
18	4.0248915392352466E-5	0.0	0.0	1.9069936112896597	0.0
19	4.0248915392352466E-5	0.0	0.0	1.9871694507512259	0.0
20	4.0248915392352466E-5	0.0	0.0	2.0708469458519265	0.0
21	4.0248915392352466E-5	0.0	0.0	2.1773858248954836	2.0124457696176233E-5
22	4.0248915392352466E-5	0.0	0.0	2.3047736421122793	2.0124457696176233E-5
23	4.0248915392352466E-5	0.0	0.0	2.4382189210956238	2.0124457696176233E-5
24	4.0248915392352466E-5	0.0	0.0	2.5461061388048245	2.0124457696176233E-5
25	4.0248915392352466E-5	0.0	0.0	2.646647929454921	2.0124457696176233E-5
26	4.0248915392352466E-5	0.0	0.0	2.7436075666350983	2.0124457696176233E-5
27	4.0248915392352466E-5	0.0	0.0	2.8441292328274983	2.0124457696176233E-5
28	4.0248915392352466E-5	0.0	0.0	2.947428074181971	2.0124457696176233E-5
29	4.0248915392352466E-5	0.0	0.0	3.066504490370246	2.0124457696176233E-5
30	4.0248915392352466E-5	0.0	0.0	3.2077379344820107	2.0124457696176233E-5
31	4.0248915392352466E-5	0.0	0.0	3.3309801134133936	2.0124457696176233E-5
32	4.0248915392352466E-5	0.0	0.0	3.4541619189716886	2.0124457696176233E-5
33	4.0248915392352466E-5	0.0	0.0	3.5746671716563916	2.0124457696176233E-5
34	4.0248915392352466E-5	0.0	0.0	3.6998211740689118	2.0124457696176233E-5
35	4.0248915392352466E-5	0.0	0.0	3.84214133889627	2.0124457696176233E-5
36	4.0248915392352466E-5	0.0	0.0	3.9761903516105	2.0124457696176233E-5
37	4.0248915392352466E-5	0.0	0.0	4.11529060320647	2.0124457696176233E-5
38	4.0248915392352466E-5	0.0	0.0	4.271617390590367	2.0124457696176233E-5
39	4.0248915392352466E-5	0.0	0.0	4.50349139216571	2.0124457696176233E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	26085	0.0	42.429558	1
TAGGGAC	5670	0.0	39.642857	5
TACGGGA	1960	0.0	39.60459	4
TAGGGAT	8810	0.0	38.742905	5
TACGGGT	595	0.0	38.571426	4
AGGGCGA	4755	0.0	38.51735	6
ACGGGAT	2405	0.0	38.076923	5
AAGGGAT	9575	0.0	37.926895	5
ATAGGGA	7745	0.0	37.853455	4
TCGTAAG	245	0.0	37.65306	1
GGGCGAT	9175	0.0	37.56948	7
TCGATAG	240	0.0	37.500004	1
CGCGTAA	30	1.1404679E-4	37.500004	31
TAAGGGA	7075	0.0	37.494698	4
AGGGATT	10760	0.0	37.304836	6
GGCGATA	2070	0.0	37.282608	8
GTAGGGA	7020	0.0	37.083332	4
ACGGGTA	510	0.0	37.058823	5
AGGGATC	6780	0.0	36.96903	6
GCGATAA	615	0.0	36.951218	9
>>END_MODULE