FastQCFastQC Report
Thu 26 May 2016
SRR1547392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547392_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1780939
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT646163.628198382987851No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTT21070.11830837552549527No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT18520.10399008612872197No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT18220.10230558149380749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG253.8916725E-545.01
CGTTTTT285100.044.4554561
GCGTAAG1150.043.0434761
TCGTTAG650.041.538461
CCGGGTA603.6379788E-1241.2499965
GTTTTTT317000.040.2870642
TCTAGCG451.929402E-840.01
GGCGATC4400.039.8863648
CGGGTAA2050.039.5121966
GTTAGCG800.039.3751
GGCGATA7450.039.2617458
ACGGGTA3050.039.0983625
TACGATG356.250224E-638.5714261
TAGGGCG6650.038.5714265
TGGGCGA8800.038.3522726
GGGCGAT31200.038.293277
AGCGGGT6700.038.2835854
GACCGAT10700.038.2710279
AGGGCGA16550.038.2024156
GGACCGA11150.038.139018
Produced by FastQC (version 0.11.5)