##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547392_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1780939
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.378272922317947	31.0	31.0	33.0	30.0	34.0
2	31.76268530252861	31.0	31.0	34.0	30.0	34.0
3	31.629657725503233	31.0	31.0	34.0	30.0	34.0
4	35.53326363227488	37.0	35.0	37.0	33.0	37.0
5	35.516239466932895	37.0	35.0	37.0	33.0	37.0
6	35.573884338542754	37.0	35.0	37.0	33.0	37.0
7	36.00163902300977	37.0	35.0	37.0	35.0	37.0
8	36.026842581357364	37.0	35.0	37.0	35.0	37.0
9	37.804651366498234	39.0	38.0	39.0	35.0	39.0
10	36.971234837352654	39.0	37.0	39.0	32.0	39.0
11	36.8341234595907	39.0	37.0	39.0	32.0	39.0
12	36.61111020646973	39.0	35.0	39.0	32.0	39.0
13	36.47748631480359	39.0	35.0	39.0	32.0	39.0
14	37.64086641934395	40.0	36.0	41.0	32.0	41.0
15	37.756895098596864	40.0	36.0	41.0	32.0	41.0
16	37.834972449926696	40.0	36.0	41.0	33.0	41.0
17	37.76469098604725	40.0	36.0	41.0	33.0	41.0
18	37.47037770524425	39.0	36.0	40.0	32.0	41.0
19	37.21215100573349	38.0	36.0	40.0	32.0	41.0
20	36.866479424618134	38.0	35.0	40.0	32.0	41.0
21	36.75013518149695	38.0	35.0	40.0	32.0	41.0
22	36.67138290531006	38.0	35.0	40.0	32.0	41.0
23	36.57108469183953	38.0	35.0	40.0	31.0	41.0
24	36.43318103539762	38.0	35.0	40.0	31.0	41.0
25	36.31982061148641	38.0	35.0	40.0	31.0	41.0
26	36.12802066774887	37.0	35.0	40.0	31.0	41.0
27	36.05923279797904	37.0	35.0	40.0	31.0	41.0
28	36.01781195200959	37.0	35.0	40.0	30.0	41.0
29	35.97358415981682	37.0	35.0	40.0	30.0	41.0
30	35.88633411924833	37.0	35.0	40.0	30.0	41.0
31	35.68780851000511	37.0	35.0	40.0	30.0	41.0
32	35.29672043792628	37.0	34.0	40.0	27.0	41.0
33	34.77902836649655	37.0	34.0	40.0	24.0	41.0
34	34.19485900415455	37.0	33.0	40.0	21.0	41.0
35	33.77923612206819	37.0	33.0	40.0	18.0	41.0
36	33.42142824655982	37.0	33.0	40.0	15.0	41.0
37	33.32640983211665	37.0	33.0	40.0	15.0	41.0
38	33.226230095472104	37.0	33.0	40.0	15.0	41.0
39	33.09873050115697	36.0	33.0	40.0	15.0	41.0
40	32.9989898587206	36.0	32.0	40.0	14.0	41.0
41	32.94808412865348	36.0	32.0	40.0	12.0	41.0
42	32.854868695671215	36.0	32.0	40.0	12.0	41.0
43	32.73314189873994	36.0	32.0	40.0	10.0	41.0
44	32.63142308636062	36.0	32.0	40.0	10.0	41.0
45	32.50926730224898	36.0	31.0	40.0	10.0	41.0
46	32.43230116247665	35.0	31.0	40.0	10.0	41.0
47	32.40896459676609	35.0	31.0	40.0	10.0	41.0
48	32.287125499525814	35.0	31.0	40.0	10.0	41.0
49	32.157957122619024	35.0	31.0	40.0	10.0	41.0
50	32.04754570482201	35.0	31.0	40.0	10.0	41.0
51	31.663857661604357	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	4.0
13	5.0
14	32.0
15	52.0
16	185.0
17	468.0
18	1017.0
19	2122.0
20	3839.0
21	6163.0
22	9350.0
23	14610.0
24	22407.0
25	36950.0
26	53719.0
27	58546.0
28	53586.0
29	48783.0
30	49885.0
31	55678.0
32	65919.0
33	82317.0
34	121699.0
35	175009.0
36	159246.0
37	164778.0
38	229387.0
39	365080.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.367585301910957	21.966614241139084	25.756637369387718	24.909163087562234
2	39.271923406697255	22.945872935569383	26.184726147273995	11.59747751045937
3	24.018060135692462	22.27684384473584	41.90486030122312	11.800235718348578
4	20.773198857456656	25.492113991551648	41.05845287233308	12.676234278658619
5	19.484496661592566	28.955174770163378	39.7794085030425	11.78092006520156
6	18.3883333454992	36.38350330920935	36.40562647008123	8.822536875210211
7	79.05492551962757	2.3202366841312365	17.652822471741032	0.9720153245001653
8	79.65460916965714	1.8722707515529728	17.422887589075202	1.0502324897146955
9	75.13979984715928	3.6622253766131236	19.163317777868865	2.0346569983587313
10	30.960409087565605	22.1672387431574	34.056416306229465	12.815935863047528
11	26.221729099087614	23.18670094820766	34.053833399122595	16.53773655358213
12	25.848330571681565	23.022068695222014	34.98935112319961	16.140249609896802
13	20.043415299457195	28.428486320980113	35.64625178066178	15.881846598900918
14	16.986769339095837	29.659803058948114	36.20084685663013	17.15258074532592
15	16.332283138277052	26.45666134550369	41.89503402418612	15.316021492033135
16	17.743898022335408	24.756322367020996	41.63685561380822	15.862923996835377
17	17.964231228582225	25.81115916940446	36.298941176536644	19.925668425476672
18	18.394734463111874	26.260865756772127	38.88100603108809	16.4633937490279
19	20.64388505164972	28.62265355523126	34.40999382909802	16.323467564021
20	21.973745310760222	26.84898247497528	35.67836966903414	15.498902545230353
21	19.422226140255226	28.75718932540643	36.30012032978109	15.520464204557257
22	19.806630097942715	25.948277846686494	36.093993112622044	18.151098942748742
23	17.332541990489286	29.086509981532217	36.05648480941795	17.524463218560545
24	16.995641063506387	27.779615135611046	40.11754473342433	15.107199067458236
25	16.741280863634298	29.638129099312216	37.38044930230625	16.240140734747232
26	17.093791533567405	31.559755836668185	34.99884049930964	16.347612130454777
27	15.916547394380157	31.20819971936153	36.42758118048962	16.4476717057687
28	15.19535481001876	29.71387565772887	38.882858986186505	16.207910546065868
29	15.730016581140625	27.542549183324077	39.07034435205248	17.657089883482815
30	16.462776097328433	30.404410257734828	37.074318659987796	16.05849498494895
31	19.221040136691936	29.839539703493497	35.07992132240352	15.859498837411051
32	19.796916121214707	28.465096221712255	35.368252365746386	16.369735291326652
33	19.328679982862973	30.1940717789885	33.651910593231996	16.82533764491653
34	17.08778346703621	30.47802311028059	34.066691784502446	18.36750163818076
35	17.98152547616735	30.79757363952387	33.3680154120944	17.85288547221438
36	20.538996563049043	31.561328040994102	30.413113531681883	17.486561864274968
37	17.69049922540862	33.219498253449444	32.21772334706579	16.872279174076148
38	17.871415023198438	32.78787201582985	31.770936567731965	17.569776393239746
39	17.67017286948065	34.09566526422297	30.42838637370511	17.80577549259127
40	20.392893861047458	31.930178405885883	30.098055014798376	17.578872718268286
41	17.20974160260402	30.96860701012219	31.609055672316682	20.21259571495711
42	18.487494518341165	31.40652206504546	29.69012414237658	20.41585927423679
43	18.296190941969375	32.67147274555726	30.031629382028246	19.00070693044512
44	18.45116536838151	33.16177589462637	30.029944877393326	18.357113859598783
45	17.104796963848845	35.03135143876349	28.313546954724444	19.550304642663225
46	18.38973709936163	34.40364886163984	29.071405589972482	18.135208449026045
47	18.060921794626317	32.39869529501011	30.66573307676456	18.874649833599015
48	18.641065190890874	31.08775763796514	31.565202401654407	18.70597476948958
49	17.99371005969323	31.605967413819343	31.167041656115117	19.233280870372315
50	17.713913839833932	32.934367768912914	31.059626410562068	18.292091980691087
51	16.71343038700371	33.781168248884434	29.911973402794818	19.593427961317037
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1087.0
1	1521.5
2	1956.0
3	43633.5
4	85311.0
5	55471.0
6	25631.0
7	26154.5
8	26678.0
9	29987.5
10	33297.0
11	35828.0
12	38359.0
13	38464.0
14	38569.0
15	36171.0
16	33773.0
17	30812.0
18	27851.0
19	25178.5
20	22506.0
21	21190.0
22	19874.0
23	19140.5
24	18407.0
25	19057.0
26	21273.0
27	22839.0
28	26123.0
29	29407.0
30	32857.5
31	36308.0
32	41980.5
33	47653.0
34	54809.5
35	61966.0
36	64650.5
37	67335.0
38	73316.0
39	79297.0
40	86629.5
41	93962.0
42	103531.5
43	113101.0
44	115918.0
45	118735.0
46	120869.5
47	123004.0
48	119471.5
49	115939.0
50	112022.5
51	108106.0
52	100591.0
53	93076.0
54	84582.0
55	76088.0
56	67056.0
57	58024.0
58	52085.0
59	46146.0
60	40476.5
61	34807.0
62	29531.0
63	24255.0
64	20007.5
65	15760.0
66	12228.5
67	8697.0
68	6934.5
69	5172.0
70	4104.5
71	3037.0
72	2370.0
73	1703.0
74	1550.5
75	1301.5
76	1205.0
77	852.5
78	500.0
79	358.5
80	217.0
81	164.0
82	111.0
83	77.5
84	44.0
85	32.5
86	21.0
87	15.5
88	10.0
89	7.0
90	4.0
91	4.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1780939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.754767798886494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.29827319296965	23.15780907706901
2	7.702958095891714	4.738053752074051
3	3.6977054112573247	3.4116621393571536
4	2.2156324767228446	2.7256504939713153
5	1.5433870998863226	2.373325594040035
6	1.0554663430765308	1.9476373380515146
7	0.8410586414374431	1.8106594255858852
8	0.6660322350618568	1.638693338872064
9	0.569458904484401	1.5762218740583434
>10	5.8277102912152925	39.560123354927214
>50	0.5334507596763677	10.377390483399955
>100	0.0453496587194022	2.022394970944291
>500	0.0025913923374825247	0.5706922413509035
>1k	7.403978107092928E-4	0.4112969021207259
>5k	0.0	0.0
>10k+	1.850994526773232E-4	3.6783890141775535
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64616	3.628198382987851	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTT	2107	0.11830837552549527	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1852	0.10399008612872197	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	1822	0.10230558149380749	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03543074748770171	0.0
2	0.0	0.0	0.0	0.10881899941547689	0.0
3	0.0	0.0	0.0	0.18810301756545283	0.0
4	0.0	0.0	0.0	0.263737275673114	0.0
5	0.0	0.0	0.0	0.4559392545168588	0.0
6	0.0	0.0	0.0	0.8425892183842344	0.0
7	0.0	0.0	0.0	1.1144682664594352	0.0
8	0.0	0.0	0.0	1.4745030570951616	0.0
9	0.0	0.0	0.0	1.6782158176108222	0.0
10	0.0	0.0	0.0	1.8870943923402206	0.0
11	0.0	0.0	0.0	2.0822723293723144	0.0
12	0.0	0.0	0.0	2.2160219973845257	0.0
13	0.0	0.0	0.0	2.3087820526138176	0.0
14	0.0	0.0	0.0	2.3666167117458823	0.0
15	0.0	0.0	0.0	2.4138951418324828	0.0
16	0.0	0.0	0.0	2.4875079943782463	0.0
17	0.0	0.0	0.0	2.568869568244617	0.0
18	0.0	0.0	0.0	2.656070758178691	0.0
19	0.0	0.0	0.0	2.718790480752008	0.0
20	0.0	0.0	0.0	2.784710762131662	0.0
21	0.0	0.0	0.0	2.8780323189059254	0.0
22	0.0	0.0	0.0	2.973599881860075	0.0
23	0.0	0.0	0.0	3.0889322991972215	0.0
24	0.0	0.0	0.0	3.1856790153958108	0.0
25	5.61501544971501E-5	0.0	0.0	3.273104805947874	0.0
26	5.61501544971501E-5	0.0	0.0	3.3556455330586843	0.0
27	5.61501544971501E-5	0.0	0.0	3.4473948855070273	0.0
28	5.61501544971501E-5	0.0	0.0	3.546724508812486	0.0
29	5.61501544971501E-5	0.0	0.0	3.654869706373997	0.0
30	5.61501544971501E-5	0.0	0.0	3.7823867072370247	0.0
31	5.61501544971501E-5	0.0	0.0	3.8967084217932224	0.0
32	5.61501544971501E-5	0.0	0.0	4.012153139439363	0.0
33	5.61501544971501E-5	0.0	0.0	4.125913352450589	0.0
34	5.61501544971501E-5	0.0	0.0	4.246411584001473	0.0
35	5.61501544971501E-5	0.0	0.0	4.377802945524804	0.0
36	5.61501544971501E-5	0.0	0.0	4.500491033101078	0.0
37	5.61501544971501E-5	0.0	0.0	4.6300855896805	0.0
38	5.61501544971501E-5	0.0	0.0	4.767653468198517	0.0
39	5.61501544971501E-5	0.0	0.0	4.956205686999947	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGCG	25	3.8916725E-5	45.0	1
CGTTTTT	28510	0.0	44.455456	1
GCGTAAG	115	0.0	43.043476	1
TCGTTAG	65	0.0	41.53846	1
CCGGGTA	60	3.6379788E-12	41.249996	5
GTTTTTT	31700	0.0	40.287064	2
TCTAGCG	45	1.929402E-8	40.0	1
GGCGATC	440	0.0	39.886364	8
CGGGTAA	205	0.0	39.512196	6
GTTAGCG	80	0.0	39.375	1
GGCGATA	745	0.0	39.261745	8
ACGGGTA	305	0.0	39.098362	5
TACGATG	35	6.250224E-6	38.571426	1
TAGGGCG	665	0.0	38.571426	5
TGGGCGA	880	0.0	38.352272	6
GGGCGAT	3120	0.0	38.29327	7
AGCGGGT	670	0.0	38.283585	4
GACCGAT	1070	0.0	38.271027	9
AGGGCGA	1655	0.0	38.202415	6
GGACCGA	1115	0.0	38.13901	8
>>END_MODULE