FastQCFastQC Report
Thu 26 May 2016
SRR1547390_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547390_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2533070
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142730.5634664655931341No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26590.10497143782051029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG302.166542E-645.00000410
ACGTTAG750.045.0000041
TCGATAG1650.040.909091
CGTTTTT82000.040.856711
CGTTAGG4200.039.6428532
ACGGGTA6400.039.3755
GTTACGC403.4604273E-739.37540
TTAATCG2750.039.27272820
CGGGTAT4450.038.9325876
AGGGTAC14950.038.6789326
ACACGAC9550.038.63874426
CACGACC9550.038.63874427
TATCGCG1300.038.076921
TACGGGT6750.038.04
TAATCGT2850.037.89473721
AACACGT9450.037.6190541
TTACGCG601.5643309E-1037.5000041
CGGTCGA301.1403099E-437.50000431
TCGGTAA301.1403099E-437.50000437
CGCAATC1750.037.28571320
Produced by FastQC (version 0.11.5)