##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547390_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2533070
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266710750196403	31.0	31.0	33.0	30.0	34.0
2	31.6741519184231	31.0	31.0	34.0	30.0	34.0
3	31.80026015862175	31.0	31.0	34.0	30.0	34.0
4	35.58607223645616	37.0	35.0	37.0	33.0	37.0
5	35.479484578002186	37.0	35.0	37.0	33.0	37.0
6	35.50941466283995	37.0	35.0	37.0	33.0	37.0
7	35.90499117671442	37.0	35.0	37.0	35.0	37.0
8	35.90343693620784	37.0	35.0	37.0	35.0	37.0
9	37.65178222473126	39.0	37.0	39.0	35.0	39.0
10	36.770323757337934	39.0	37.0	39.0	32.0	39.0
11	36.595566644427514	39.0	35.0	39.0	32.0	39.0
12	36.105422668935326	38.0	35.0	39.0	32.0	39.0
13	35.85261994338885	38.0	35.0	39.0	31.0	39.0
14	36.88365106372899	39.0	35.0	41.0	31.0	41.0
15	37.07928205695066	39.0	35.0	41.0	32.0	41.0
16	37.189705772047354	39.0	35.0	41.0	32.0	41.0
17	37.113791170397974	39.0	35.0	41.0	32.0	41.0
18	37.013217952918794	39.0	35.0	41.0	32.0	41.0
19	36.96655718160177	38.0	35.0	41.0	31.0	41.0
20	36.83509614815224	38.0	35.0	41.0	31.0	41.0
21	36.697471842467834	38.0	35.0	40.0	31.0	41.0
22	36.58043046579842	38.0	35.0	40.0	31.0	41.0
23	36.49648805599529	38.0	35.0	40.0	31.0	41.0
24	36.40327744594504	38.0	35.0	40.0	30.0	41.0
25	36.30987221040082	38.0	35.0	40.0	30.0	41.0
26	36.080172281066055	38.0	35.0	40.0	30.0	41.0
27	36.074683289447194	38.0	35.0	40.0	30.0	41.0
28	36.14675393889628	38.0	35.0	40.0	30.0	41.0
29	36.18975748794941	38.0	35.0	40.0	30.0	41.0
30	36.18428073444476	38.0	35.0	40.0	30.0	41.0
31	36.09919504790629	38.0	35.0	40.0	30.0	41.0
32	35.957570852759694	38.0	35.0	40.0	30.0	41.0
33	35.79284741440229	38.0	34.0	40.0	29.0	41.0
34	35.62722151381525	38.0	34.0	40.0	28.0	41.0
35	35.50715613859862	38.0	34.0	40.0	27.0	41.0
36	35.286841263762945	38.0	34.0	40.0	27.0	41.0
37	35.24024957857461	38.0	34.0	40.0	27.0	41.0
38	35.163732940660935	38.0	34.0	40.0	27.0	41.0
39	35.04198818035032	38.0	34.0	40.0	26.0	41.0
40	34.923246495359386	37.0	34.0	40.0	25.0	41.0
41	34.927995673234456	37.0	34.0	40.0	25.0	41.0
42	34.85396337250846	37.0	34.0	40.0	25.0	41.0
43	34.74616414074621	37.0	33.0	40.0	25.0	41.0
44	34.65594752612443	37.0	33.0	40.0	24.0	41.0
45	34.490077652808644	37.0	33.0	40.0	24.0	41.0
46	34.41229496223949	36.0	33.0	40.0	24.0	41.0
47	34.38385674300355	36.0	33.0	40.0	24.0	41.0
48	34.27894689053204	36.0	33.0	40.0	23.0	41.0
49	34.144007469197454	36.0	33.0	40.0	24.0	41.0
50	33.9985440591851	36.0	33.0	40.0	24.0	41.0
51	33.51170634842306	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	6.0
12	6.0
13	10.0
14	30.0
15	100.0
16	226.0
17	572.0
18	1190.0
19	2344.0
20	4043.0
21	6378.0
22	9567.0
23	13862.0
24	19727.0
25	27710.0
26	37724.0
27	45999.0
28	52305.0
29	59880.0
30	70719.0
31	85664.0
32	104587.0
33	133740.0
34	209921.0
35	277751.0
36	203788.0
37	254109.0
38	363974.0
39	547020.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.9759540794372	23.22513787617397	29.76494925130375	14.033958793085072
2	30.539227103870008	25.69486828236093	30.52588361158594	13.240021002183122
3	28.823325056157152	25.76438866671667	31.781790475588913	13.630495801537265
4	25.137165573789904	29.163426198249553	31.532527723276498	14.16688050468404
5	23.126561839980734	32.33795355043485	30.49745960435361	14.038025005230805
6	21.738128042257024	41.59916622912119	26.490543095927077	10.172162632694715
7	90.71190294780642	2.735376440445783	5.503519444784391	1.049201166963408
8	91.57172916658442	1.977047614159893	5.16870832626023	1.2825148929954562
9	87.0384947909059	3.790815097885175	7.255938446233228	1.9147516649757015
10	40.35044432250194	25.99604432566017	21.825768731223377	11.82774262061451
11	36.523349137607724	25.29077364620796	21.912975164523683	16.272902051660633
12	33.24775864859637	22.84504573501719	26.023283999257817	17.88391161712862
13	24.213227427587867	29.58354881625853	26.829657293323912	19.37356646282969
14	20.560545109294257	31.186702301949808	28.85324921932674	19.39950336942919
15	20.74463003391142	24.71352943266471	36.83206543838109	17.709775095042772
16	22.80821295897863	22.930830967955877	35.6276376096989	18.633318463366585
17	22.8104237150967	23.382417382859533	28.40047847078841	25.406680431255353
18	24.698764740019026	24.467030125499885	31.098943179619987	19.735261954861098
19	27.224869427216774	25.727634846253757	26.89380869853577	20.1536870279937
20	29.16236029797834	25.936472343835742	26.306418693522087	18.594748664663825
21	26.040338403597218	27.166521256814853	28.330168530676215	18.462971808911714
22	25.88846735384336	23.420079192442373	28.450891605837974	22.240561847876293
23	23.864243783235363	26.805970620630305	28.042375457448866	21.28741013868547
24	23.036039272503324	25.095003296395284	32.876430576336226	18.992526854765167
25	21.89986853896655	26.472106969013886	30.986194617598407	20.64182987442116
26	21.75103727887504	30.00876406889664	27.272834939421337	20.96736371280699
27	20.692795698500237	30.044136166785755	28.86860607878977	20.394462055924233
28	19.23977623989862	28.554678710023808	32.57407019940231	19.631474850675268
29	20.5721121011263	25.738096460026767	31.7998713024117	21.889920136435233
30	22.216046141638408	28.169849234328304	29.95436367727698	19.65974094675631
31	25.43028814837332	26.301089192166028	27.65833553750982	20.610287121950837
32	26.973632785513228	25.717962788237198	27.500029608340864	19.808374817908703
33	25.893559988472486	26.646677746765786	26.963052738376753	20.49670952638498
34	21.766828394004115	27.394702870429953	29.396187235252086	21.442281500313847
35	22.083913985795892	27.2369101525027	29.638936152573752	21.040239709127658
36	25.981634933104893	27.9796057748108	26.198565377190526	19.840193914893785
37	22.93462083558686	29.844536471554278	27.74787116029166	19.4729715325672
38	22.814450449454615	30.04638640069165	26.16860173623311	20.970561413620626
39	22.433134496875333	29.749750302992023	27.161112799883146	20.6560024002495
40	24.468017070195454	26.54387758727552	26.872095915233295	22.116009427295733
41	20.83669223511391	25.627558654123256	28.02307081920358	25.512678291559254
42	22.22875798931731	26.746990805623216	26.389243092374077	24.635008112685398
43	21.79473918999475	26.362319241079007	27.929903239942043	23.913038328984197
44	21.29522674067436	28.506042075426265	27.467578866750625	22.731152317148755
45	20.394462055924233	30.22494443501364	26.17144413695634	23.209149372105784
46	22.47059891751906	29.041202967150532	26.836013217163362	21.652184898167047
47	22.09007252069623	26.777586091185796	28.502410118946575	22.6299312691714
48	22.535697789638657	25.826684615900863	30.292293541039133	21.34532405342134
49	22.75495742320583	25.704658773740956	29.88618553770721	21.654198265346004
50	21.529211589099393	28.72909157662441	28.43940356958157	21.30229326469462
51	20.665319158175652	29.31130999143332	27.098540506184193	22.924830344206832
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1428.0
1	2465.0
2	3502.0
3	13748.0
4	23994.0
5	16728.5
6	9463.0
7	9714.5
8	9966.0
9	10850.0
10	11734.0
11	12011.5
12	12289.0
13	12077.5
14	11866.0
15	11466.5
16	11067.0
17	10790.5
18	10514.0
19	10158.0
20	9802.0
21	10305.5
22	10809.0
23	11072.5
24	11336.0
25	14054.5
26	18697.0
27	20621.0
28	26304.0
29	31987.0
30	36519.0
31	41051.0
32	49529.5
33	58008.0
34	65195.5
35	72383.0
36	76694.0
37	81005.0
38	93593.5
39	106182.0
40	122745.5
41	139309.0
42	163207.0
43	187105.0
44	190338.5
45	193572.0
46	195130.0
47	196688.0
48	195897.0
49	195106.0
50	188059.0
51	181012.0
52	175543.0
53	170074.0
54	156761.5
55	143449.0
56	135570.5
57	127692.0
58	121853.0
59	116014.0
60	106008.0
61	96002.0
62	83671.0
63	71340.0
64	61193.0
65	51046.0
66	43554.0
67	36062.0
68	29084.5
69	22107.0
70	18770.5
71	15434.0
72	12925.0
73	10416.0
74	8692.5
75	5515.0
76	4061.0
77	3095.5
78	2130.0
79	1500.5
80	871.0
81	680.5
82	490.0
83	331.5
84	173.0
85	128.0
86	83.0
87	54.5
88	26.0
89	26.5
90	27.0
91	23.0
92	19.0
93	15.5
94	12.0
95	6.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2533070.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.036961620940005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.42869568020436	15.501549545072535
2	6.191957947321517	2.3575213160326833
3	1.947519599118144	1.1122456759332169
4	0.9624597770714847	0.732892393512333
5	0.6164147141713211	0.5867331628131071
6	0.4399857869646214	0.5025595524122749
7	0.3654484237338209	0.4869919331937638
8	0.296862457922016	0.4521087374527468
9	0.26300881691985645	0.45061998783049284
>10	4.315544534956135	21.410682467065083
>50	2.125096030661675	29.087104260380265
>100	1.0447018960805026	26.422498080040558
>500	0.0018853648973302022	0.22125189252988586
>1k	2.0948498859224468E-4	0.10603979492375026
>5k	0.0	0.0
>10k+	2.0948498859224468E-4	0.5692012008073288
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14273	0.5634664655931341	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2659	0.10497143782051029	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.895557564536314E-5	0.0	0.0	0.0287003517470895	0.0
2	7.895557564536314E-5	0.0	0.0	0.08902241154014694	0.0
3	7.895557564536314E-5	0.0	0.0	0.12514458739790058	0.0
4	7.895557564536314E-5	0.0	0.0	0.22778683573687264	0.0
5	7.895557564536314E-5	0.0	0.0	0.361142803001891	0.0
6	7.895557564536314E-5	0.0	0.0	0.6032995535062198	0.0
7	7.895557564536314E-5	0.0	0.0	0.7122187701089981	0.0
8	7.895557564536314E-5	0.0	0.0	0.9640081008420612	0.0
9	7.895557564536314E-5	0.0	0.0	1.0509776674154288	0.0
10	7.895557564536314E-5	0.0	0.0	1.212481297398019	0.0
11	1.184333634680447E-4	0.0	0.0	1.405409246487464	0.0
12	1.184333634680447E-4	0.0	0.0	1.5608727749331839	0.0
13	1.184333634680447E-4	0.0	0.0	1.6388019280951573	0.0
14	1.184333634680447E-4	0.0	0.0	1.6755952263458964	0.0
15	1.184333634680447E-4	0.0	0.0	1.7157836143493863	0.0
16	1.184333634680447E-4	0.0	0.0	1.7971473350519331	0.0
17	1.184333634680447E-4	0.0	0.0	1.892249325916773	0.0
18	1.184333634680447E-4	0.0	0.0	2.013011878866356	0.0
19	1.184333634680447E-4	0.0	0.0	2.0819795741925806	0.0
20	1.184333634680447E-4	0.0	0.0	2.163106428168191	0.0
21	1.184333634680447E-4	0.0	0.0	2.267998910413056	0.0
22	1.184333634680447E-4	0.0	0.0	2.3846952512169026	0.0
23	1.184333634680447E-4	0.0	0.0	2.5114189501277107	0.0
24	1.184333634680447E-4	0.0	0.0	2.6135874650128104	0.0
25	1.184333634680447E-4	0.0	0.0	2.709952745087976	0.0
26	1.184333634680447E-4	0.0	0.0	2.7981066452960244	0.0
27	1.184333634680447E-4	0.0	0.0	2.8858657676258455	0.0
28	1.184333634680447E-4	0.0	0.0	2.979625513704714	0.0
29	1.184333634680447E-4	0.0	0.0	3.0842416514348203	0.0
30	1.184333634680447E-4	0.0	0.0	3.2187029967588736	0.0
31	1.184333634680447E-4	0.0	0.0	3.333464925959409	0.0
32	1.184333634680447E-4	0.0	0.0	3.448542677462526	0.0
33	1.184333634680447E-4	0.0	0.0	3.562475573118785	0.0
34	1.5791115129072628E-4	0.0	0.0	3.686198960155069	0.0
35	1.5791115129072628E-4	0.0	0.0	3.82077873884259	0.0
36	1.5791115129072628E-4	0.0	0.0	3.9404753915209607	0.0
37	1.5791115129072628E-4	0.0	0.0	4.0716600804557315	0.0
38	1.5791115129072628E-4	0.0	0.0	4.216306695038037	0.0
39	1.5791115129072628E-4	0.0	0.0	4.425657403861718	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	30	2.166542E-6	45.000004	10
ACGTTAG	75	0.0	45.000004	1
TCGATAG	165	0.0	40.90909	1
CGTTTTT	8200	0.0	40.85671	1
CGTTAGG	420	0.0	39.642853	2
ACGGGTA	640	0.0	39.375	5
GTTACGC	40	3.4604273E-7	39.375	40
TTAATCG	275	0.0	39.272728	20
CGGGTAT	445	0.0	38.932587	6
AGGGTAC	1495	0.0	38.678932	6
ACACGAC	955	0.0	38.638744	26
CACGACC	955	0.0	38.638744	27
TATCGCG	130	0.0	38.07692	1
TACGGGT	675	0.0	38.0	4
TAATCGT	285	0.0	37.894737	21
AACACGT	945	0.0	37.61905	41
TTACGCG	60	1.5643309E-10	37.500004	1
CGGTCGA	30	1.1403099E-4	37.500004	31
TCGGTAA	30	1.1403099E-4	37.500004	37
CGCAATC	175	0.0	37.285713	20
>>END_MODULE