FastQCFastQC Report
Thu 26 May 2016
SRR1547389_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547389_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1695040
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132440.7813384934868793No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20700.12212101189352463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTACG406.8175723E-945.01
AATGCGT207.0342363E-445.028
GTATGCG800.045.01
TTACGCG207.0342363E-445.01
CGGTCTA207.0342363E-445.031
GCGCTTA207.0342363E-445.08
GCGTAAA207.0342363E-445.045
TACCGTT207.0342363E-445.014
TCGTTAG302.1661144E-644.9999961
TTATACG302.1661144E-644.9999961
CTACGTT302.1661144E-644.99999621
CGGGTAT1400.043.3928576
TACGTAG1250.043.21
GCGATAC1050.042.8571439
CGTTTTT71450.042.1973421
CGTTAGG2300.042.065222
TCGTAAG700.041.7857131
ACGGGAT5050.040.99015
GTAATCG556.184564E-1140.9090921
TAAGGGA18150.040.6611564