FastQCFastQC Report
Thu 26 May 2016
SRR1547388_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547388_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3555977
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226320.6364495608379919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG1050.042.8571431
CGTTTTT125550.040.913981
CGCGTAA403.4609548E-739.37531
GGGCGAT75400.039.2407157
CACGACC13650.038.5714327
ACGGGAC9100.038.5714265
AGGGCGA42800.038.32366
ACGGGTA4000.038.255
GACCGAT23000.038.0543489
AACACGT13550.037.8597841
CGAGGGT7850.037.8343964
ACACGAC13950.037.74193226
ACGGGAT12600.037.55
ACGACCA14000.037.4464328
TAGGGAT43100.037.3259855
CGTAAGG6350.037.2047232
ATAGGGA46800.036.9711534
GCGATGT7950.036.7924549
GCTACGA3550.036.76056310
GGCGATG37600.036.502668
Produced by FastQC (version 0.11.5)