##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547388_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3555977
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27997087720196	31.0	31.0	33.0	30.0	34.0
2	31.68567710083614	31.0	31.0	34.0	30.0	34.0
3	31.801299614705044	31.0	31.0	34.0	30.0	34.0
4	35.582246454349956	37.0	35.0	37.0	33.0	37.0
5	35.47484924677522	37.0	35.0	37.0	33.0	37.0
6	35.50263176617847	37.0	35.0	37.0	33.0	37.0
7	35.899430733100914	37.0	35.0	37.0	35.0	37.0
8	35.8969883663477	37.0	35.0	37.0	35.0	37.0
9	37.65052108042319	39.0	37.0	39.0	35.0	39.0
10	36.873260991283125	39.0	37.0	39.0	32.0	39.0
11	36.61297499955708	39.0	35.0	39.0	32.0	39.0
12	36.14765477954441	38.0	35.0	39.0	32.0	39.0
13	35.93119303077607	38.0	35.0	39.0	31.0	39.0
14	36.85755391556245	39.0	35.0	41.0	31.0	41.0
15	37.050155836216035	39.0	35.0	41.0	31.0	41.0
16	37.16037449061116	39.0	35.0	41.0	32.0	41.0
17	37.08580651674631	39.0	35.0	41.0	32.0	41.0
18	36.976549060919126	39.0	35.0	41.0	31.0	41.0
19	36.9208571933958	39.0	35.0	41.0	31.0	41.0
20	36.792459849993406	38.0	35.0	41.0	31.0	41.0
21	36.6800980433788	38.0	35.0	41.0	31.0	41.0
22	36.60655735399863	38.0	35.0	40.0	31.0	41.0
23	36.5048350987647	38.0	35.0	40.0	30.0	41.0
24	36.39253825320017	38.0	35.0	40.0	30.0	41.0
25	36.26611870661706	38.0	35.0	40.0	30.0	41.0
26	36.051059385367225	38.0	35.0	40.0	30.0	41.0
27	36.07345576194672	38.0	35.0	40.0	30.0	41.0
28	36.12339843592914	38.0	35.0	40.0	30.0	41.0
29	36.141717452053264	38.0	35.0	40.0	30.0	41.0
30	36.11771814047166	38.0	35.0	40.0	30.0	41.0
31	36.02723836515253	38.0	35.0	40.0	30.0	41.0
32	35.90026960241869	38.0	34.0	40.0	29.0	41.0
33	35.74776664753456	38.0	34.0	40.0	29.0	41.0
34	35.54724622797054	38.0	34.0	40.0	27.0	41.0
35	35.432027260018835	38.0	34.0	40.0	27.0	41.0
36	35.2105848828606	38.0	34.0	40.0	26.0	41.0
37	35.17848821856834	38.0	34.0	40.0	26.0	41.0
38	35.08178230624101	38.0	34.0	40.0	26.0	41.0
39	34.948154332831734	38.0	34.0	40.0	25.0	41.0
40	34.827811315989955	37.0	34.0	40.0	24.0	41.0
41	34.78930009952258	37.0	34.0	40.0	24.0	41.0
42	34.71230297608786	37.0	33.0	40.0	24.0	41.0
43	34.608836333868304	37.0	33.0	40.0	24.0	41.0
44	34.51914312156687	37.0	33.0	40.0	24.0	41.0
45	34.36854681568526	37.0	33.0	40.0	23.0	41.0
46	34.30294712254888	36.0	33.0	40.0	23.0	41.0
47	34.25352413696714	36.0	33.0	40.0	23.0	41.0
48	34.13961507625049	36.0	33.0	40.0	23.0	41.0
49	34.0093895432957	36.0	33.0	40.0	23.0	41.0
50	33.89753083329842	36.0	33.0	40.0	23.0	41.0
51	33.431024722600846	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	2.0
12	5.0
13	19.0
14	56.0
15	157.0
16	441.0
17	1004.0
18	2066.0
19	3852.0
20	6635.0
21	10176.0
22	15340.0
23	21424.0
24	29835.0
25	41477.0
26	55961.0
27	67199.0
28	75356.0
29	86789.0
30	100753.0
31	120670.0
32	147757.0
33	187360.0
34	287526.0
35	353531.0
36	286755.0
37	359586.0
38	514942.0
39	779068.0
40	229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.46588012239674	23.93584097984886	28.40513310406676	14.193145793687641
2	31.257204419488655	25.410934885124398	29.36635979366571	13.965500901721242
3	28.81863409127787	25.675981593806707	31.80824847854753	13.697135836367897
4	25.385906601758112	28.609746351002833	30.994773025809785	15.009574021429273
5	23.271297873973875	32.38814536764439	29.926908976070433	14.413647782311303
6	21.999636105632856	41.149394385846705	26.699722748487968	10.151246760032475
7	90.43269964906972	2.739134701939861	5.731673742546704	1.0964919064437144
8	91.16015092336086	2.1610657211787365	5.501357292243454	1.1774260632169444
9	86.55972746730363	4.174520813829786	7.016299599238128	2.2494521196284456
10	41.10383728578672	30.296371433223555	16.197433222993286	12.402358057996437
11	34.66588788397675	24.783765474298626	23.892758586458797	16.657588055265823
12	32.15948809567666	22.07809555573616	26.9504555288181	18.811960819769084
13	25.130421259755053	27.494750387868088	28.27791068389925	19.09691766847761
14	21.185288881227297	28.311459832276753	30.71355635877285	19.789694927723097
15	21.70390865857681	24.50901679060354	35.488615365060014	18.29845918575964
16	25.300332369978772	23.62903359611156	32.72968300976075	18.340951024148918
17	25.606408590381776	23.867167869758436	27.835191284983	22.691232254876788
18	26.194938831156673	24.210983366877795	29.84515929096279	19.748918511002742
19	27.442415966132515	25.78278768394734	26.801945006955897	19.972851342964255
20	28.448103010790003	26.506808114900632	26.73293443686503	18.312154437444335
21	26.79634879528186	26.447499519822543	28.48272078250225	18.27343090239335
22	26.17269459279405	23.298322795676125	28.95159333145293	21.577389280076893
23	24.199200388528947	26.202391072833148	28.969281859809552	20.62912667882835
24	23.931538364843192	25.188239406497846	31.743428036795514	19.136794191863444
25	23.56339762602514	25.98891950088541	29.50232805217807	20.945354820911383
26	22.10115532243319	28.562698802607557	27.40897930442182	21.927166570537434
27	21.46540880326279	27.973493641831766	29.227326273482646	21.3337712814228
28	19.876281539503772	27.913172666752345	30.98166832912586	21.22887746461802
29	21.339564344763758	25.986669767549113	30.597048293619444	22.076717594067677
30	23.205521295553936	26.836900238668587	29.97263480613064	19.98494365964684
31	25.546762535303237	26.107396082708075	28.340397027314857	20.005444354673834
32	26.900398962085525	25.604805655379657	27.38428848105598	20.110506901478832
33	26.334169203006656	26.298229712959337	27.020084775576446	20.347516308457564
34	22.175537130864456	26.93431369213018	29.283653971890146	21.60649520511522
35	22.0982025474293	28.138483460382336	29.18671858676251	20.576595405425852
36	25.780116125610487	28.257831813872812	26.090382474352335	19.871669586164366
37	22.81252100336982	29.48568002548948	27.162830355764395	20.53896861537631
38	23.480466830915947	28.522372332554458	27.089742144001494	20.9074186925281
39	22.84831988508362	28.525943784225827	26.55467119163032	22.071065139060238
40	25.284612358291408	26.37502998472712	26.429304801465253	21.911052855516218
41	21.67007829353227	25.734840242217537	27.96958473015995	24.625496734090238
42	22.487040832941272	26.42548025479355	27.215192899166667	23.87228601309851
43	22.117128429120886	26.120613266058808	27.918825121759788	23.84343318306052
44	22.417636559516556	26.512544934908185	28.03808911025015	23.031729395325108
45	21.39901354817537	28.59996001098995	26.324720322994217	23.676306117840472
46	22.520674346318888	28.316184272282975	27.10264998901849	22.060491392379646
47	21.622665163469843	27.132402712390995	29.176201083415332	22.06873104072383
48	22.723206589918888	26.927873830455034	28.951059019785564	21.397860559840517
49	22.68917937320742	26.168954411122456	29.01019888486343	22.131667330806696
50	21.66226047018864	28.32200545729064	27.896215301730017	22.119518770790698
51	20.91458971753754	28.75848184619867	27.264855762565393	23.062072673698395
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1764.0
1	3183.5
2	4603.0
3	20640.0
4	36677.0
5	25135.0
6	13593.0
7	13535.0
8	13477.0
9	14037.0
10	14597.0
11	15123.5
12	15650.0
13	15639.5
14	15629.0
15	15146.5
16	14664.0
17	13899.0
18	13134.0
19	12888.5
20	12643.0
21	14176.0
22	15709.0
23	17563.0
24	19417.0
25	21813.0
26	27569.5
27	30930.0
28	39785.0
29	48640.0
30	53710.5
31	58781.0
32	69824.5
33	80868.0
34	93623.0
35	106378.0
36	111066.5
37	115755.0
38	131603.5
39	147452.0
40	164440.5
41	181429.0
42	204021.5
43	226614.0
44	235343.0
45	244072.0
46	248591.0
47	253110.0
48	255252.0
49	257394.0
50	253064.0
51	248734.0
52	242534.5
53	236335.0
54	222884.0
55	209433.0
56	204392.5
57	199352.0
58	190450.0
59	181548.0
60	164082.5
61	146617.0
62	130161.0
63	113705.0
64	97527.0
65	81349.0
66	70789.0
67	60229.0
68	49685.0
69	39141.0
70	32507.5
71	25874.0
72	21676.0
73	17478.0
74	15006.5
75	10620.5
76	8706.0
77	6254.5
78	3803.0
79	3079.5
80	2356.0
81	1517.5
82	679.0
83	542.5
84	406.0
85	323.0
86	240.0
87	183.0
88	126.0
89	80.5
90	35.0
91	60.5
92	86.0
93	49.0
94	12.0
95	8.0
96	4.0
97	2.0
98	0.0
99	2.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3555977.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.439288699712787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.18458827210675	15.781706493887999
2	6.196507886637564	2.409114114767895
3	1.9482212642170964	1.1361610681810668
4	1.0547328409169352	0.8201302478261017
5	0.6874237800609764	0.6681514659826592
6	0.4769119774006905	0.5562497767825753
7	0.3796391416057772	0.5165940412770093
8	0.3224440899891342	0.5014467003851959
9	0.2672428192051717	0.4675509283909033
>10	4.42691727950825	22.21182056312923
>50	2.09373246518279	29.203906700521287
>100	0.9594437062715064	24.6549179559243
>500	0.0014629845982324708	0.19836467527871338
>1k	5.851938392929883E-4	0.2302452202231489
>5k	0.0	0.0
>10k+	1.4629845982324708E-4	0.6436400474419843
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22632	0.6364495608379919	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.6243333407387056E-5	0.0	0.0	0.016226201688031166	0.0
2	5.6243333407387056E-5	0.0	0.0	0.049887836732352316	0.0
3	5.6243333407387056E-5	0.0	0.0	0.07334130676323272	0.0
4	5.6243333407387056E-5	0.0	0.0	0.13931473685009774	0.0
5	5.6243333407387056E-5	0.0	0.0	0.2437867286543192	0.0
6	5.6243333407387056E-5	0.0	0.0	0.4488780439243561	0.0
7	5.6243333407387056E-5	0.0	0.0	0.5694918724164977	0.0
8	8.436500011108059E-5	0.0	0.0	0.8181998927439632	0.0
9	8.436500011108059E-5	0.0	8.436500011108059E-5	0.9441287162431028	0.0
10	8.436500011108059E-5	0.0	8.436500011108059E-5	1.1212389731429646	0.0
11	8.436500011108059E-5	0.0	8.436500011108059E-5	1.3032986433826765	0.0
12	8.436500011108059E-5	0.0	8.436500011108059E-5	1.4347111918890365	0.0
13	8.436500011108059E-5	0.0	8.436500011108059E-5	1.5004596486422719	0.0
14	8.436500011108059E-5	0.0	8.436500011108059E-5	1.5426140270311084	0.0
15	8.436500011108059E-5	0.0	8.436500011108059E-5	1.581196953748576	0.0
16	8.436500011108059E-5	0.0	8.436500011108059E-5	1.6448362854990344	0.0
17	8.436500011108059E-5	0.0	8.436500011108059E-5	1.7194993105973408	0.0
18	8.436500011108059E-5	0.0	8.436500011108059E-5	1.8119914723857888	0.0
19	8.436500011108059E-5	0.0	8.436500011108059E-5	1.8764182108039507	0.0
20	8.436500011108059E-5	0.0	8.436500011108059E-5	1.9516999125697383	0.0
21	8.436500011108059E-5	0.0	8.436500011108059E-5	2.050435084366406	0.0
22	8.436500011108059E-5	0.0	8.436500011108059E-5	2.1576067561741823	0.0
23	8.436500011108059E-5	0.0	8.436500011108059E-5	2.2740304563274734	0.0
24	8.436500011108059E-5	0.0	8.436500011108059E-5	2.365172778114144	0.0
25	8.436500011108059E-5	0.0	8.436500011108059E-5	2.4528561348962605	0.0
26	8.436500011108059E-5	0.0	8.436500011108059E-5	2.5353369833381936	0.0
27	8.436500011108059E-5	0.0	8.436500011108059E-5	2.630191365129752	0.0
28	8.436500011108059E-5	0.0	8.436500011108059E-5	2.725608180255384	0.0
29	8.436500011108059E-5	0.0	8.436500011108059E-5	2.8480780387499696	0.0
30	8.436500011108059E-5	0.0	8.436500011108059E-5	2.995041868943472	0.0
31	1.1248666681477411E-4	0.0	8.436500011108059E-5	3.108709645759801	0.0
32	1.1248666681477411E-4	0.0	8.436500011108059E-5	3.2214212859082045	0.0
33	1.1248666681477411E-4	0.0	8.436500011108059E-5	3.3386042710624957	0.0
34	1.1248666681477411E-4	0.0	8.436500011108059E-5	3.4611866162238956	0.0
35	1.1248666681477411E-4	0.0	8.436500011108059E-5	3.598982783071994	0.0
36	1.4060833351846764E-4	0.0	8.436500011108059E-5	3.7220713182340606	0.0
37	1.4060833351846764E-4	0.0	8.436500011108059E-5	3.8516559584046806	0.0
38	1.4060833351846764E-4	0.0	8.436500011108059E-5	4.041983398655278	0.0
39	1.4060833351846764E-4	0.0	8.436500011108059E-5	4.467407972548754	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCG	105	0.0	42.857143	1
CGTTTTT	12555	0.0	40.91398	1
CGCGTAA	40	3.4609548E-7	39.375	31
GGGCGAT	7540	0.0	39.240715	7
CACGACC	1365	0.0	38.57143	27
ACGGGAC	910	0.0	38.571426	5
AGGGCGA	4280	0.0	38.3236	6
ACGGGTA	400	0.0	38.25	5
GACCGAT	2300	0.0	38.054348	9
AACACGT	1355	0.0	37.85978	41
CGAGGGT	785	0.0	37.834396	4
ACACGAC	1395	0.0	37.741932	26
ACGGGAT	1260	0.0	37.5	5
ACGACCA	1400	0.0	37.44643	28
TAGGGAT	4310	0.0	37.325985	5
CGTAAGG	635	0.0	37.204723	2
ATAGGGA	4680	0.0	36.971153	4
GCGATGT	795	0.0	36.792454	9
GCTACGA	355	0.0	36.760563	10
GGCGATG	3760	0.0	36.50266	8
>>END_MODULE