FastQCFastQC Report
Thu 26 May 2016
SRR1547387_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547387_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3371028
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371231.1012367740641726No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT41820.12405711254845703No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA40830.12112032294006457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT188300.041.5108871
TCGTTGA10450.037.67942424
TCGTAAG1200.037.4999961
TAACGGG8950.037.45813
GGGCGAT54550.037.2868927
TACGGGA11450.037.1397364
TTAATCG10950.036.986320
AGGGATT41100.036.8430676
AAGGGAT47400.036.693045
TCGATAG1350.036.6666641
TAATCGT11050.036.65158521
CGTTAGG6100.036.5163962
GTTAATC12650.036.4624540
GGATGCC32150.036.46198
TAGGGAC30950.036.421655
GCATATC13200.036.30681628
CGTTGAT10800.036.2525
TATGGGA32700.036.123854
TTAGGGA40400.036.0891084
AGGGCGA27950.036.06446
Produced by FastQC (version 0.11.5)