##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547387_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3371028
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23922494859135	31.0	31.0	33.0	30.0	34.0
2	31.64432125749178	31.0	31.0	34.0	30.0	34.0
3	31.72316664234174	31.0	31.0	34.0	30.0	34.0
4	35.53654315538168	37.0	35.0	37.0	33.0	37.0
5	35.44268513936995	37.0	35.0	37.0	33.0	37.0
6	35.474220920146614	37.0	35.0	37.0	33.0	37.0
7	35.85028810202704	37.0	35.0	37.0	35.0	37.0
8	35.83209483872575	37.0	35.0	37.0	35.0	37.0
9	37.561306521334146	39.0	37.0	39.0	35.0	39.0
10	36.88082656091851	39.0	37.0	39.0	32.0	39.0
11	36.563401727900214	39.0	35.0	39.0	32.0	39.0
12	36.18160424653844	38.0	35.0	39.0	31.0	39.0
13	36.039127826882485	38.0	35.0	39.0	31.0	39.0
14	37.09827981256756	39.0	35.0	41.0	31.0	41.0
15	37.234168627492856	39.0	35.0	41.0	31.0	41.0
16	37.324103804536776	39.0	35.0	41.0	32.0	41.0
17	37.24568232598483	39.0	35.0	41.0	32.0	41.0
18	37.07068941581025	39.0	35.0	41.0	31.0	41.0
19	36.977174618543664	39.0	35.0	41.0	31.0	41.0
20	36.79555257328032	38.0	35.0	41.0	31.0	41.0
21	36.649951290822855	38.0	35.0	40.0	31.0	41.0
22	36.54440485216973	38.0	35.0	40.0	30.0	41.0
23	36.43266060086122	38.0	35.0	40.0	30.0	41.0
24	36.294884231160346	38.0	35.0	40.0	30.0	41.0
25	36.172953176301114	38.0	35.0	40.0	30.0	41.0
26	35.93513551355848	38.0	34.0	40.0	30.0	41.0
27	35.91774497275015	38.0	34.0	40.0	30.0	41.0
28	35.94929439921591	38.0	35.0	40.0	30.0	41.0
29	35.942660517800505	38.0	35.0	40.0	30.0	41.0
30	35.917230886245974	38.0	35.0	40.0	29.0	41.0
31	35.843809366163676	38.0	34.0	40.0	29.0	41.0
32	35.64758554363832	38.0	34.0	40.0	28.0	41.0
33	35.40773407993051	38.0	34.0	40.0	27.0	41.0
34	35.12622381065954	38.0	34.0	40.0	25.0	41.0
35	34.949436492369685	38.0	34.0	40.0	24.0	41.0
36	34.68894414404152	38.0	33.0	40.0	24.0	41.0
37	34.65138557140433	38.0	33.0	40.0	23.0	41.0
38	34.556300926601615	37.0	33.0	40.0	23.0	41.0
39	34.377289064344765	37.0	33.0	40.0	23.0	41.0
40	34.22541462129653	37.0	33.0	40.0	23.0	41.0
41	34.16551508916568	37.0	33.0	40.0	23.0	41.0
42	34.06439193029545	37.0	33.0	40.0	23.0	41.0
43	33.93784358955191	36.0	33.0	40.0	22.0	41.0
44	33.81686180002065	36.0	33.0	40.0	22.0	41.0
45	33.662750057252566	36.0	33.0	40.0	22.0	41.0
46	33.56613205229977	36.0	32.0	40.0	22.0	41.0
47	33.52406862239056	35.0	32.0	40.0	22.0	41.0
48	33.39806047294772	35.0	32.0	40.0	21.0	41.0
49	33.24164913492264	35.0	32.0	39.0	20.0	41.0
50	33.111818116016835	35.0	32.0	39.0	20.0	41.0
51	32.63249519137782	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	3.0
12	9.0
13	21.0
14	62.0
15	173.0
16	491.0
17	1067.0
18	2388.0
19	4316.0
20	7423.0
21	11520.0
22	16546.0
23	23937.0
24	33101.0
25	47112.0
26	63580.0
27	75679.0
28	81948.0
29	88692.0
30	100787.0
31	118038.0
32	141654.0
33	177189.0
34	260316.0
35	312044.0
36	294336.0
37	364416.0
38	485292.0
39	658694.0
40	190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.928946303620144	23.030482096262624	27.552722789606023	16.487848810511217
2	33.299634414190564	25.086798448425824	28.080188001998202	13.533379135385406
3	28.12596038953103	25.089112282662736	33.15036244136804	13.634564886438202
4	24.692586356446757	28.247851990550068	32.26846528714683	14.79109636585635
5	22.481984723947708	31.743996193446034	31.3407364163098	14.433282666296455
6	22.280799803502077	40.03585849776389	27.671707265558158	10.011634433175875
7	87.10722663828363	2.966276162642375	8.23567766271891	1.6908195363550822
8	87.14457429603077	2.733528170041898	8.457865078545773	1.664032455381563
9	82.08863883658042	5.110399557642357	10.020652453791543	2.780309151985685
10	43.234615672133245	30.910600564575553	15.530930030839258	10.32385373245194
11	32.58293909157681	24.765531463992584	25.928173838959513	16.72335560547109
12	30.29271782969468	20.84598526028262	29.70693212871563	19.154364781307066
13	25.34642251562431	23.93691182630343	30.478269536770387	20.238396121301868
14	22.087564980178154	24.267256160435334	31.389979555198	22.255199304188515
15	20.68733929234643	23.442759894014525	34.941181147115955	20.92871966652309
16	24.616407813877547	22.658221764992756	32.6841545071711	20.04121591395859
17	25.28786471070546	23.19959964734793	29.418147817223705	22.0943878247229
18	24.866183253298402	23.531427208554778	30.80659668208036	20.79579285606646
19	26.920690068430165	24.959211255438994	27.4604067364614	20.659691939669443
20	28.59730028940727	24.104190175815805	28.472827873277822	18.825681661499104
21	27.468653478998096	24.367967278824146	29.045768827787843	19.11761041438991
22	27.604072110940635	22.426393373178747	28.907977032525388	21.061557483355227
23	25.654399785466037	24.082297744189606	28.90462493933601	21.358677531008347
24	23.99185055715942	23.08978151471895	32.04761277568742	20.87075515243421
25	23.620539491217514	25.05378181373753	29.276855606064384	22.04882308898057
26	23.531278885847286	25.892873034575803	27.9929149209084	22.582933158668514
27	22.389045715431614	25.524914061823278	30.07699728391458	22.00904293883053
28	21.91215854629508	24.987303576238464	31.070106804215214	22.030431073251243
29	22.869047661425533	23.462368155945306	30.890636328146787	22.77794785448237
30	23.05786246806612	24.972026337366522	29.785305847355765	22.184805347211594
31	25.033698919142765	24.21297004949232	28.250581128367962	22.50274990299695
32	26.24410120592294	24.283690316425734	27.570343527256373	21.901864950394952
33	25.469886337342796	25.265052678292793	27.27224454973379	21.992816434630626
34	23.172545585500924	25.36377627240118	29.078993114266627	22.38468502783127
35	23.868535058148435	26.046772675872166	28.362802088858352	21.721890177121043
36	25.335387306186718	26.579903815690642	26.173944565278013	21.910764312844627
37	23.820152190963707	27.482061851755606	26.85661465879251	21.841171298488177
38	23.933530068572555	26.69251041522052	27.325818711680828	22.0481408045261
39	23.55216272306252	27.111818709307666	26.852580281148658	22.483438286481157
40	25.904353212135884	24.987570557111955	27.127629909926586	21.980446320825575
41	22.36780590371839	24.86395841268598	28.652743317468737	24.11549236612689
42	23.273523684763223	24.664553364730285	27.45684699148153	24.605075959024962
43	22.866555839939625	25.417973389719695	27.904188277285147	23.81128249305553
44	23.06216382658346	25.318241201200347	28.267489917022342	23.352105055193846
45	22.168875488426675	26.31464348560736	27.211966201408	24.30451482455797
46	22.415981119112626	27.1137172399636	27.33397646059303	23.136325180330747
47	22.449146076508413	25.559710569001503	28.998246232306585	22.9928971221835
48	23.10683862608083	24.71685195139287	29.682221565647037	22.494087856879265
49	23.24741888824418	25.560422517997477	28.519757177929105	22.672401415829235
50	21.839599077788733	26.203994745816413	29.324823169668125	22.631583006726732
51	21.77445574465712	26.435823137630422	28.37876161218477	23.41095950552769
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1894.0
1	3251.5
2	4609.0
3	31220.5
4	57832.0
5	39277.0
6	20722.0
7	20543.5
8	20365.0
9	20863.5
10	21362.0
11	21362.0
12	21362.0
13	20757.0
14	20152.0
15	19589.0
16	19026.0
17	18003.0
18	16980.0
19	16624.5
20	16269.0
21	16099.5
22	15930.0
23	16793.0
24	17656.0
25	19415.5
26	26928.0
27	32681.0
28	36677.0
29	40673.0
30	47992.0
31	55311.0
32	61790.0
33	68269.0
34	79932.5
35	91596.0
36	95920.5
37	100245.0
38	105598.0
39	110951.0
40	125002.5
41	139054.0
42	153985.0
43	168916.0
44	175397.5
45	181879.0
46	193375.0
47	204871.0
48	205098.5
49	205326.0
50	204394.0
51	203462.0
52	200727.5
53	197993.0
54	196059.0
55	194125.0
56	193265.0
57	192405.0
58	188329.5
59	184254.0
60	177518.0
61	170782.0
62	162153.0
63	153524.0
64	136921.0
65	120318.0
66	106660.5
67	93003.0
68	80562.0
69	68121.0
70	56691.5
71	45262.0
72	38286.0
73	31310.0
74	25268.5
75	15447.0
76	11667.0
77	8634.5
78	5602.0
79	4133.0
80	2664.0
81	1932.0
82	1200.0
83	882.0
84	564.0
85	394.5
86	225.0
87	172.0
88	119.0
89	89.0
90	59.0
91	42.5
92	26.0
93	15.0
94	4.0
95	3.0
96	2.0
97	2.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3371028.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.221742322069943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.22594228203202	18.397644168965336
2	5.384407374815874	2.500706412300546
3	2.072283679955631	1.4436611290248156
4	1.2167145948592606	1.1301693120529392
5	0.8617995006890089	1.000624296914435
6	0.644928877678124	0.8985823328101896
7	0.5195990646427734	0.8446196912946133
8	0.47024032156902	0.8735839661538471
9	0.4439712544757326	0.9278807462857444
>10	7.518743955244392	43.459583749015934
>50	1.5383962853318	23.62198155606575
>100	0.100264951710169	3.1395715897965726
>500	0.0019341835687352266	0.29239738196323495
>1k	6.447278562449222E-4	0.35740452136328904
>5k	0.0	0.0
>10k+	1.2894557124898447E-4	1.111589145992743
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37123	1.1012367740641726	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	4182	0.12405711254845703	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	4083	0.12112032294006457	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.025392847523070115	0.0
2	0.0	0.0	0.0	0.07223315854985482	0.0
3	0.0	0.0	0.0	0.10854255734452517	0.0
4	0.0	0.0	0.0	0.18475076445523442	0.0
5	0.0	0.0	0.0	0.3257166656580723	0.0
6	0.0	0.0	0.0	0.6437798796094247	0.0
7	0.0	0.0	0.0	0.8122744753232545	0.0
8	2.9664541498913684E-5	0.0	0.0	1.1300707084011168	0.0
9	2.9664541498913684E-5	0.0	0.0	1.3126559613269306	0.0
10	2.9664541498913684E-5	0.0	0.0	1.5277832162770526	0.0
11	2.9664541498913684E-5	0.0	0.0	1.7248744299958352	0.0
12	2.9664541498913684E-5	0.0	0.0	1.8708833032534882	0.0
13	2.9664541498913684E-5	0.0	0.0	1.9662548041724957	0.0
14	2.9664541498913684E-5	0.0	0.0	2.0326143835055657	0.0
15	2.9664541498913684E-5	0.0	0.0	2.0880870761085344	0.0
16	2.9664541498913684E-5	0.0	0.0	2.155425585311068	0.0
17	2.9664541498913684E-5	0.0	0.0	2.225938200453986	0.0
18	2.9664541498913684E-5	0.0	0.0	2.3127959779628053	0.0
19	2.9664541498913684E-5	0.0	0.0	2.3831899349397276	0.0
20	2.9664541498913684E-5	0.0	0.0	2.4580929022244846	0.0
21	2.9664541498913684E-5	0.0	0.0	2.5518921824440497	0.0
22	2.9664541498913684E-5	0.0	0.0	2.6575572792631803	0.0
23	2.9664541498913684E-5	0.0	0.0	2.7692442780065902	0.0
24	2.9664541498913684E-5	0.0	0.0	2.8678492139489795	0.0
25	2.9664541498913684E-5	0.0	0.0	2.9623011140815207	0.0
26	2.9664541498913684E-5	0.0	0.0	3.0461034438159516	0.0
27	2.9664541498913684E-5	0.0	0.0	3.135097068312693	0.0
28	2.9664541498913684E-5	0.0	0.0	3.2222514912365012	0.0
29	2.9664541498913684E-5	0.0	0.0	3.324060197660773	0.0
30	2.9664541498913684E-5	0.0	0.0	3.454257870299505	0.0
31	2.9664541498913684E-5	0.0	0.0	3.5665381598728936	0.0
32	2.9664541498913684E-5	0.0	0.0	3.6854633067420384	0.0
33	2.9664541498913684E-5	0.0	0.0	3.7929676051341015	0.0
34	2.9664541498913684E-5	0.0	0.0	3.911091809382776	0.0
35	2.9664541498913684E-5	0.0	0.0	4.034436972935259	0.0
36	2.9664541498913684E-5	0.0	0.0	4.152709499891428	0.0
37	2.9664541498913684E-5	0.0	0.0	4.2751053981159455	0.0
38	2.9664541498913684E-5	0.0	0.0	4.417109558271245	0.0
39	2.9664541498913684E-5	0.0	0.0	4.644814578816907	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18830	0.0	41.510887	1
TCGTTGA	1045	0.0	37.679424	24
TCGTAAG	120	0.0	37.499996	1
TAACGGG	895	0.0	37.4581	3
GGGCGAT	5455	0.0	37.286892	7
TACGGGA	1145	0.0	37.139736	4
TTAATCG	1095	0.0	36.9863	20
AGGGATT	4110	0.0	36.843067	6
AAGGGAT	4740	0.0	36.69304	5
TCGATAG	135	0.0	36.666664	1
TAATCGT	1105	0.0	36.651585	21
CGTTAGG	610	0.0	36.516396	2
GTTAATC	1265	0.0	36.46245	40
GGATGCC	3215	0.0	36.4619	8
TAGGGAC	3095	0.0	36.42165	5
GCATATC	1320	0.0	36.306816	28
CGTTGAT	1080	0.0	36.25	25
TATGGGA	3270	0.0	36.12385	4
TTAGGGA	4040	0.0	36.089108	4
AGGGCGA	2795	0.0	36.0644	6
>>END_MODULE