FastQCFastQC Report
Thu 26 May 2016
SRR1547385_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547385_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2491372
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345341.3861438596885571No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTG207.0348737E-445.00000411
CCACGCG207.0348737E-445.00000445
TCGAATC351.2126839E-745.00000441
GTACGAC207.0348737E-445.00000433
CGTTTTT152800.043.3802381
GCGATAC800.042.1875049
ACGTGCG603.6379788E-1241.2500041
TTTGACG1800.041.251
TTACGCG501.0822987E-940.51
GCGATCG900.040.09
AGGGCGA20150.039.6401986
TACGGGA10350.039.565224
TAGGGAC26500.039.311325
AAGGGAT50750.038.7487685
ACATACG9300.038.7096817
CGAGGGA17050.038.6656884
CGGGATC8150.038.6503076
TGGGCGA10600.038.6320766
CGGTTTA356.2512263E-638.5714334
CAACCGG700.038.571432