##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547385_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2491372
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.270059228409085	31.0	31.0	33.0	30.0	34.0
2	31.669662740048455	31.0	31.0	34.0	30.0	34.0
3	31.708672169391	31.0	31.0	34.0	30.0	34.0
4	35.51281221752512	37.0	35.0	37.0	33.0	37.0
5	35.40529314771138	37.0	35.0	37.0	33.0	37.0
6	35.44840513580469	37.0	35.0	37.0	33.0	37.0
7	35.92884362511901	37.0	35.0	37.0	35.0	37.0
8	35.94703079267167	37.0	35.0	37.0	35.0	37.0
9	37.69228561611835	39.0	37.0	39.0	35.0	39.0
10	36.95933044121873	39.0	37.0	39.0	32.0	39.0
11	36.535989808025455	39.0	35.0	39.0	32.0	39.0
12	36.20577938581633	38.0	35.0	39.0	32.0	39.0
13	36.014316208097384	38.0	35.0	39.0	31.0	39.0
14	37.11711659278502	39.0	35.0	41.0	31.0	41.0
15	37.30967956611859	39.0	35.0	41.0	32.0	41.0
16	37.42238252657572	39.0	35.0	41.0	32.0	41.0
17	37.338141393577516	39.0	35.0	41.0	32.0	41.0
18	37.19071499559279	39.0	35.0	41.0	32.0	41.0
19	37.08851468187007	38.0	35.0	41.0	32.0	41.0
20	36.923810253948425	38.0	35.0	41.0	32.0	41.0
21	36.79028663724245	38.0	35.0	41.0	31.0	41.0
22	36.68550702183375	38.0	35.0	40.0	31.0	41.0
23	36.596217265025054	38.0	35.0	40.0	31.0	41.0
24	36.51320878616281	38.0	35.0	40.0	31.0	41.0
25	36.423335816570145	38.0	35.0	40.0	31.0	41.0
26	36.22365547979186	38.0	35.0	40.0	30.0	41.0
27	36.20545827760768	38.0	35.0	40.0	30.0	41.0
28	36.224681019133236	38.0	35.0	40.0	30.0	41.0
29	36.25649963152833	38.0	35.0	40.0	30.0	41.0
30	36.23125570970534	38.0	35.0	40.0	30.0	41.0
31	36.1467424375003	38.0	35.0	40.0	30.0	41.0
32	35.93905245784251	38.0	35.0	40.0	30.0	41.0
33	35.72248744868289	38.0	35.0	40.0	29.0	41.0
34	35.50799117915751	38.0	34.0	40.0	27.0	41.0
35	35.35140115566844	38.0	34.0	40.0	26.0	41.0
36	35.1059841725764	38.0	34.0	40.0	25.0	41.0
37	35.05618711296426	38.0	34.0	40.0	25.0	41.0
38	35.00681150787598	38.0	34.0	40.0	25.0	41.0
39	34.89724256353527	38.0	34.0	40.0	24.0	41.0
40	34.78589187002182	38.0	34.0	40.0	24.0	41.0
41	34.78570522587554	38.0	34.0	40.0	24.0	41.0
42	34.7129894692563	38.0	34.0	40.0	24.0	41.0
43	34.61079236661566	37.0	33.0	40.0	23.0	41.0
44	34.525559410637996	37.0	33.0	40.0	23.0	41.0
45	34.39037446033752	37.0	33.0	40.0	23.0	41.0
46	34.30590935436378	37.0	33.0	40.0	23.0	41.0
47	34.27877009133923	37.0	33.0	40.0	23.0	41.0
48	34.18456296370032	37.0	33.0	40.0	23.0	41.0
49	34.056509023943434	37.0	33.0	40.0	23.0	41.0
50	33.928035636588994	36.0	33.0	40.0	22.0	41.0
51	33.46790764285703	36.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	1.0
13	4.0
14	25.0
15	65.0
16	213.0
17	521.0
18	1178.0
19	2342.0
20	3985.0
21	6408.0
22	9526.0
23	14569.0
24	20968.0
25	30893.0
26	42601.0
27	50718.0
28	54457.0
29	59140.0
30	68101.0
31	81031.0
32	98914.0
33	124604.0
34	187821.0
35	259276.0
36	201484.0
37	244051.0
38	353038.0
39	575254.0
40	182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.693999932567277	24.510831782648275	29.904646917441475	15.890521367342975
2	31.893711577395912	25.774793968945627	29.69676949086688	12.634724962791585
3	26.533693081563094	25.476925966896957	34.7606860797986	13.228694871741354
4	23.588849838562847	28.216902172778696	34.18626363305039	14.007984355608075
5	21.71771216823501	32.55009689440196	32.252670416140184	13.479520521222843
6	20.877251570620526	40.50603442601105	28.473788739698445	10.142925263669976
7	88.61338250570368	2.4747408255370935	7.919812858136	0.9920638106232229
8	89.48535184629193	1.7889741074395955	7.5311916486177095	1.1944823976507724
9	84.83598595472695	4.838980288772611	8.65306345258757	1.671970303912864
10	40.26568493183675	38.58074988399966	13.321856390775846	7.83170879338774
11	27.613258879043357	25.019948847462363	32.25993548936088	15.106856784133402
12	29.442451789616324	23.213113095916626	30.501787769951655	16.84264734451539
13	21.199925181787385	30.813864810233078	31.52540046207471	16.460809545904826
14	17.1682109295601	32.758094736554796	31.405747515826622	18.667946818058486
15	16.04473358454699	27.424005728570442	40.12568175286549	16.40557893401708
16	19.265689748459884	25.09765703395559	39.07080114892517	16.565852068659357
17	19.105416613817606	25.577392697678224	31.39735856387565	23.919832124628517
18	19.697178903832906	27.124572324004603	33.940214468172556	19.238034303989927
19	23.581183380081335	27.6014180138494	29.786800204867035	19.03059840120223
20	25.778326159240773	27.100449069829796	29.994075553550413	17.127149217379017
21	22.274714494663982	28.585133011047724	31.76835896044429	17.371793533844002
22	22.421501084542975	26.068728395438335	30.33934715490099	21.170423365117692
23	18.982592724009102	30.277774655892415	30.845132722050337	19.894499898048142
24	19.50563785737337	27.10161308708615	36.27707945662068	17.11566959891979
25	18.147952212676387	29.192669741812942	33.074587014705145	19.584791030805516
26	18.395366087440976	33.568451439608374	29.47652939825927	18.559653074691372
27	18.57277837271993	32.731844140497685	30.9325945703813	17.762782916401086
28	17.174352124050525	30.204883092528934	35.108566685344464	17.51219809807608
29	17.488636783266408	27.1117681341847	34.76040511011603	20.639189972432863
30	18.48660095722357	30.354318825129283	32.41378645983017	18.745293757816977
31	22.382727268348525	28.967653164601675	29.62347654224259	19.026143024807215
32	23.356528049604798	28.898855730898475	29.962727364680987	17.78188885481574
33	21.878145856981615	30.185215214749146	29.356675759380774	18.57996316888847
34	18.764439834757717	30.09855613693981	30.932153046594408	20.20485098170807
35	18.99483497446387	29.21534800904883	31.292918119012334	20.496898897474967
36	22.805707056192333	29.274110811231722	29.1051677549559	18.81501437762004
37	19.60140838060314	32.2075547128249	30.15876392606162	18.032272980510335
38	19.317026923317755	32.107890752565254	28.726500899905755	19.848581424211236
39	19.29270297651254	32.282132094283796	29.054553073567497	19.37061185563617
40	21.885732038410964	29.544524061440846	28.86052343849092	19.70922046165727
41	17.719072061498643	29.190903646665372	29.91672861379192	23.173295678044063
42	19.74759289259091	29.685410287985896	27.780716809854166	22.786280009569023
43	20.01427325987448	30.04689785387329	28.98487259229051	20.95395629396172
44	19.27488147093248	31.425415393606414	28.99763664358434	20.302066491876765
45	18.145905147846246	33.10015525581888	27.47682802889332	21.277111567441555
46	19.76421024238853	32.24869670205814	27.83382007985961	20.153272975693714
47	19.508527831251214	30.163821380347855	29.3416639506264	20.98598683777453
48	20.18658795234112	28.47254444538993	30.470439581082232	20.87042802118672
49	19.21836642621014	29.204390191428658	30.244499817771093	21.332743564590114
50	18.447345478716144	31.986993512008645	29.198971490407693	20.366689518867513
51	17.622458629221168	31.83844082698208	28.193501412073346	22.345599131723404
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1202.0
1	1950.0
2	2698.0
3	25203.0
4	47708.0
5	31356.0
6	15004.0
7	15020.5
8	15037.0
9	16286.0
10	17535.0
11	18954.5
12	20374.0
13	20397.5
14	20421.0
15	20542.5
16	20664.0
17	20134.0
18	19604.0
19	19289.5
20	18975.0
21	19168.5
22	19362.0
23	20206.0
24	21050.0
25	23696.5
26	30108.5
27	33874.0
28	40710.5
29	47547.0
30	54215.0
31	60883.0
32	69249.5
33	77616.0
34	90726.5
35	103837.0
36	106490.0
37	109143.0
38	117970.0
39	126797.0
40	145837.0
41	164877.0
42	180724.5
43	196572.0
44	199563.5
45	202555.0
46	205637.0
47	208719.0
48	200941.0
49	193163.0
50	180034.5
51	166906.0
52	152233.0
53	137560.0
54	120861.0
55	104162.0
56	94935.5
57	85709.0
58	76740.5
59	67772.0
60	61415.5
61	55059.0
62	46319.0
63	37579.0
64	28554.0
65	19529.0
66	15047.0
67	10565.0
68	7980.0
69	5395.0
70	4663.0
71	3931.0
72	3035.5
73	2140.0
74	1850.5
75	1383.5
76	1206.0
77	811.0
78	416.0
79	316.0
80	216.0
81	127.5
82	39.0
83	41.0
84	43.0
85	29.0
86	15.0
87	9.5
88	4.0
89	2.5
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2491372.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.487865744949122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3160987575461	17.836497802728225
2	6.96384671791385	3.1320410012170252
3	2.58927355869875	1.7468170849489242
4	1.477496042852937	1.3290293060148177
5	0.9771789464012748	1.0987334477731352
6	0.6942924765818845	0.9367893600660991
7	0.5121609406707316	0.8062184531527186
8	0.43473567895529447	0.7821022066308773
9	0.3442540807492317	0.6967385595035638
>10	4.397985100692584	23.748620606901707
>50	1.6223942871332369	26.210421103008358
>100	0.6677581387614827	19.794361212670047
>500	0.0019841431049982287	0.29878601178670766
>1k	3.6075329181785976E-4	0.182046106556984
>5k	0.0	0.0
>10k+	1.8037664590892988E-4	1.4007977370407498
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34534	1.3861438596885571	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.027705216242296E-5	0.0	0.0	0.04728318372366712	0.0
2	8.027705216242296E-5	0.0	0.0	0.1532087540519842	0.0
3	8.027705216242296E-5	0.0	0.0	0.2234511746941043	0.0
4	8.027705216242296E-5	0.0	0.0	0.35474429350574704	0.0
5	8.027705216242296E-5	0.0	0.0	0.6295326430577208	0.0
6	8.027705216242296E-5	0.0	0.0	1.0399089337120269	0.0
7	8.027705216242296E-5	0.0	0.0	1.2552922646638078	0.0
8	8.027705216242296E-5	0.0	0.0	1.6750609704211175	0.0
9	8.027705216242296E-5	0.0	0.0	1.8404316978757087	0.0
10	8.027705216242296E-5	0.0	0.0	2.057862093657631	0.0
11	8.027705216242296E-5	0.0	0.0	2.359141870423205	0.0
12	8.027705216242296E-5	0.0	0.0	2.590821442963957	0.0
13	8.027705216242296E-5	0.0	0.0	2.7167761378067987	0.0
14	8.027705216242296E-5	0.0	0.0	2.7833258140494475	0.0
15	8.027705216242296E-5	0.0	0.0	2.854250589634948	0.0
16	8.027705216242296E-5	0.0	0.0	2.973100765361415	0.0
17	8.027705216242296E-5	0.0	0.0	3.128436861295704	0.0
18	8.027705216242296E-5	0.0	0.0	3.3103848000218354	0.0
19	8.027705216242296E-5	0.0	0.0	3.437904897381844	0.0
20	8.027705216242296E-5	0.0	0.0	3.566147488211315	0.0
21	8.027705216242296E-5	0.0	0.0	3.7366960855303826	0.0
22	8.027705216242296E-5	0.0	0.0	3.937228161832115	0.0
23	8.027705216242296E-5	0.0	0.0	4.156665483918098	0.0
24	8.027705216242296E-5	0.0	0.0	4.320470808855522	0.0
25	8.027705216242296E-5	0.0	0.0	4.4602331566703	0.0
26	8.027705216242296E-5	0.0	0.0	4.601279937319678	0.0
27	8.027705216242296E-5	0.0	0.0	4.739476882617288	0.0
28	8.027705216242296E-5	0.0	0.0	4.888992892269801	0.0
29	1.2041557824363443E-4	0.0	0.0	5.05127295321614	0.0
30	1.2041557824363443E-4	0.0	0.0	5.24578425060569	0.0
31	1.2041557824363443E-4	0.0	0.0	5.417537003707194	0.0
32	1.2041557824363443E-4	0.0	0.0	5.582988008213948	0.0
33	1.2041557824363443E-4	0.0	0.0	5.748840397981514	0.0
34	1.2041557824363443E-4	0.0	0.0	5.917542623100846	0.0
35	1.2041557824363443E-4	0.0	0.0	6.107919652304032	0.0
36	1.2041557824363443E-4	0.0	0.0	6.293680751007878	0.0
37	1.2041557824363443E-4	0.0	0.0	6.488633572184322	0.0
38	1.2041557824363443E-4	0.0	0.0	6.673672177418707	0.0
39	1.2041557824363443E-4	0.0	0.0	6.862644358209051	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTG	20	7.0348737E-4	45.000004	11
CCACGCG	20	7.0348737E-4	45.000004	45
TCGAATC	35	1.2126839E-7	45.000004	41
GTACGAC	20	7.0348737E-4	45.000004	33
CGTTTTT	15280	0.0	43.380238	1
GCGATAC	80	0.0	42.187504	9
ACGTGCG	60	3.6379788E-12	41.250004	1
TTTGACG	180	0.0	41.25	1
TTACGCG	50	1.0822987E-9	40.5	1
GCGATCG	90	0.0	40.0	9
AGGGCGA	2015	0.0	39.640198	6
TACGGGA	1035	0.0	39.56522	4
TAGGGAC	2650	0.0	39.31132	5
AAGGGAT	5075	0.0	38.748768	5
ACATACG	930	0.0	38.70968	17
CGAGGGA	1705	0.0	38.665688	4
CGGGATC	815	0.0	38.650307	6
TGGGCGA	1060	0.0	38.632076	6
CGGTTTA	35	6.2512263E-6	38.57143	34
CAACCGG	70	0.0	38.57143	2
>>END_MODULE