FastQCFastQC Report
Thu 26 May 2016
SRR1547384_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547384_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1778880
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT412772.320392606583918No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17880.10051268213707501No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGA207.0343306E-445.022
TCGCCGA351.2124292E-745.028
AACCCGT207.0343306E-445.031
GCGAACG700.045.01
CGCGCGT207.0343306E-445.045
AGACGCG453.8562575E-1045.01
ACGGCGC253.8916703E-545.041
CGACTAT207.0343306E-445.037
ATCGCCG207.0343306E-445.01
CGTTTTT172200.043.7195131
TATTGCG1100.042.9545441
CGACGGT556.184564E-1140.90909228
CGACCCG556.184564E-1140.90909232
TACGGGA6600.040.909094
AGGGCGA18300.040.327876
GCGATCG950.040.2631579
TGGGCGA8050.039.409946
GCGATAT1600.039.3759
TCGTCCC4000.038.812538
GGCACCG8250.038.7272728