##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547384_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1778880
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46801245727649	31.0	31.0	33.0	30.0	34.0
2	31.866211323979133	31.0	31.0	34.0	30.0	34.0
3	31.834824159021405	31.0	31.0	34.0	30.0	34.0
4	35.65734113599568	37.0	35.0	37.0	33.0	37.0
5	35.59907750944414	37.0	35.0	37.0	33.0	37.0
6	35.65314186454398	37.0	35.0	37.0	33.0	37.0
7	36.06398689062781	37.0	35.0	37.0	35.0	37.0
8	36.08876933801044	37.0	35.0	37.0	35.0	37.0
9	37.867620075553155	39.0	38.0	39.0	35.0	39.0
10	37.2321303291959	39.0	37.0	39.0	33.0	39.0
11	36.851860721352764	39.0	37.0	39.0	32.0	39.0
12	36.55295466810578	39.0	35.0	39.0	32.0	39.0
13	36.39673614858788	39.0	35.0	39.0	32.0	39.0
14	37.52824080320202	40.0	36.0	41.0	32.0	41.0
15	37.691074721172875	40.0	36.0	41.0	33.0	41.0
16	37.781117332254006	40.0	36.0	41.0	33.0	41.0
17	37.69705320201475	40.0	36.0	41.0	33.0	41.0
18	37.49751304191401	39.0	36.0	41.0	32.0	41.0
19	37.34959468879295	39.0	36.0	41.0	32.0	41.0
20	37.125456466990464	39.0	35.0	41.0	32.0	41.0
21	37.00163642291779	38.0	35.0	41.0	32.0	41.0
22	36.91081860496492	38.0	35.0	41.0	32.0	41.0
23	36.83973230347185	38.0	35.0	40.0	32.0	41.0
24	36.72369299784134	38.0	35.0	40.0	31.0	41.0
25	36.63881599658212	38.0	35.0	40.0	31.0	41.0
26	36.46229200395754	38.0	35.0	40.0	31.0	41.0
27	36.443363801942795	38.0	35.0	40.0	31.0	41.0
28	36.439867781975174	38.0	35.0	40.0	31.0	41.0
29	36.46324203993524	38.0	35.0	40.0	31.0	41.0
30	36.40129913203813	38.0	35.0	40.0	31.0	41.0
31	36.274815052167654	38.0	35.0	40.0	30.0	41.0
32	36.00094216585717	38.0	35.0	40.0	30.0	41.0
33	35.68663822180248	38.0	35.0	40.0	29.0	41.0
34	35.339275274329914	38.0	34.0	40.0	26.0	41.0
35	35.07918465551358	38.0	34.0	40.0	24.0	41.0
36	34.815759354200395	38.0	34.0	40.0	23.0	41.0
37	34.75352693829826	38.0	34.0	40.0	23.0	41.0
38	34.67568694909156	38.0	34.0	40.0	23.0	41.0
39	34.5660179438748	38.0	34.0	40.0	23.0	41.0
40	34.46585660640403	38.0	34.0	40.0	22.0	41.0
41	34.44096116657672	38.0	34.0	40.0	22.0	41.0
42	34.365385523475446	38.0	34.0	40.0	22.0	41.0
43	34.274976389638425	37.0	33.0	40.0	21.0	41.0
44	34.196424154524195	37.0	33.0	40.0	21.0	41.0
45	34.05212830545062	37.0	33.0	40.0	20.0	41.0
46	33.975676830365174	37.0	33.0	40.0	20.0	41.0
47	33.94579285842777	37.0	33.0	40.0	20.0	41.0
48	33.8554674851592	37.0	33.0	40.0	20.0	41.0
49	33.741321505666484	36.0	33.0	40.0	20.0	41.0
50	33.62690344486418	36.0	33.0	40.0	20.0	41.0
51	33.19068402590394	36.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	3.0
14	14.0
15	49.0
16	124.0
17	335.0
18	727.0
19	1431.0
20	2619.0
21	4326.0
22	6668.0
23	10136.0
24	15886.0
25	24889.0
26	35922.0
27	41006.0
28	40528.0
29	40745.0
30	43875.0
31	51273.0
32	63739.0
33	81386.0
34	127772.0
35	184520.0
36	145504.0
37	173157.0
38	250119.0
39	431986.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.508286112610183	23.02173277567908	28.458018528512323	19.011962583198418
2	34.8709300233855	25.05329195898543	28.022969508904477	12.052808508724592
3	25.615218564490018	25.03344801223242	36.81591788091384	12.535415542363735
4	22.409549829105956	27.12487632667746	36.88281390537866	13.58275993883792
5	20.728716945493794	31.840371469688794	34.91157357438388	12.51933801043353
6	20.732989296636088	39.22209480122324	31.00130419140133	9.043611710739341
7	85.78408886490377	2.3961706242129877	11.055439377585897	0.7643011332973557
8	86.57453004137435	1.8341878035617918	10.775431732325957	0.8158504227379025
9	82.17569481921208	4.78891212448282	11.629339809318223	1.406053246986868
10	38.30230257240511	37.51270462313366	16.33196168375607	7.853031120705163
11	25.655299964022305	24.673502428494334	34.654445493793844	15.016752113689513
12	26.73721667566109	23.790868411584817	33.86102491455298	15.610889998201117
13	20.866612700125923	28.94787731606404	34.380677729807516	15.804832254002518
14	16.806136445403848	31.99777388019428	34.494906907717215	16.701182766684656
15	15.599703184025904	27.184633027522935	42.048704803022126	15.166958985429032
16	19.176897823349524	25.542644810217663	40.3804641122504	14.899993254182405
17	18.797220723151646	25.1290699766145	34.30591158481741	21.767797715416442
18	19.861317233315347	26.295983989926246	36.73890313005936	17.103795646699048
19	22.401960784313726	27.496289800323797	33.18307024644721	16.918679168915276
20	24.520372369131138	27.56133072495053	32.772643461054145	15.145653444864186
21	21.375472207231518	27.475265335491994	34.839393326137795	16.309869131138694
22	21.563568087785573	25.199619985608923	34.2780288720993	18.958783054506206
23	18.93151870840079	28.737126731426514	34.125854470228454	18.205500089944234
24	18.40006071235834	27.483360316603704	38.17362610181687	15.942952869221083
25	17.892831444504406	28.725771271811478	36.67374977513941	16.707647508544703
26	17.8137367332254	32.45412844036697	32.59556574923547	17.136569077172155
27	16.80697967260299	31.999910055765422	35.20125022486059	15.991860046771004
28	15.448540654794026	29.982629519697788	38.44604470228458	16.1227851232236
29	16.808047760388558	28.003856359057384	37.53507825148408	17.653017629069975
30	17.842237812556217	29.831972926785394	35.853402140672785	16.47238711998561
31	21.361699496312287	28.828644990106135	32.83313095880554	16.97652455477604
32	21.624449091563232	29.53639368591473	32.90171343766865	15.937443784853391
33	21.078206511962584	29.76569526893326	32.61304865983091	16.54304955927325
34	18.171602356538948	30.001517808958443	33.15670534268753	18.670174491815075
35	18.59242894405469	30.1290699766145	32.775116927504946	18.50338415182587
36	21.72074563770462	29.924334412664148	31.215315254542187	17.139604695089044
37	18.92853930563051	32.49685195178989	31.650870210469513	16.923738532110093
38	19.153175031480483	32.766572225220365	29.909549829105952	18.1707029141932
39	18.918533009534087	32.53440366972477	30.78718069796726	17.75988262277388
40	20.629384781435512	30.364723871199857	30.311431912214427	18.694459435150208
41	17.487801313185823	30.40154479222882	31.00214741860047	21.10850647598489
42	19.010051268213708	30.214460784313722	30.14733989926246	20.62814804821011
43	19.1804393775859	30.914957726209753	30.357191041554238	19.547411854650118
44	18.720543263176832	32.11599433351322	30.43701654973916	18.726445853570787
45	17.77078836121605	33.55825013491635	29.20292543622954	19.468036067638064
46	19.45415092642562	32.62114364094262	30.02985024284943	17.894855189782337
47	18.72110541464292	31.49043218204713	30.930473106673862	18.857989296636084
48	19.82297850332794	29.75939917251304	31.95724275948912	18.460379564669903
49	19.023036967080408	29.677156413023926	32.31437758589674	18.985429033998923
50	18.046579870480304	32.348837470768125	30.96380868861306	18.640773970138515
51	17.673536157582298	32.606640133117466	30.14531615398453	19.574507555315705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	825.0
1	1348.0
2	1871.0
3	28880.0
4	55889.0
5	36050.5
6	16212.0
7	16369.0
8	16526.0
9	17967.0
10	19408.0
11	20793.0
12	22178.0
13	22424.5
14	22671.0
15	21916.5
16	21162.0
17	20234.0
18	19306.0
19	18391.5
20	17477.0
21	17576.5
22	17676.0
23	18311.5
24	18947.0
25	20449.5
26	24799.5
27	27647.0
28	35307.0
29	42967.0
30	45863.0
31	48759.0
32	56241.0
33	63723.0
34	69244.0
35	74765.0
36	77915.0
37	81065.0
38	89435.5
39	97806.0
40	105945.5
41	114085.0
42	124667.5
43	135250.0
44	136276.0
45	137302.0
46	134156.5
47	131011.0
48	125111.5
49	119212.0
50	112385.0
51	105558.0
52	95398.5
53	85239.0
54	75803.5
55	66368.0
56	59400.5
57	52433.0
58	47045.0
59	41657.0
60	36433.0
61	31209.0
62	26522.0
63	21835.0
64	17342.5
65	12850.0
66	9693.5
67	6537.0
68	5327.5
69	4118.0
70	3147.0
71	2176.0
72	1685.0
73	1194.0
74	1164.5
75	726.5
76	318.0
77	310.0
78	302.0
79	185.0
80	68.0
81	71.5
82	75.0
83	60.0
84	45.0
85	46.0
86	47.0
87	26.5
88	6.0
89	6.5
90	7.0
91	9.0
92	11.0
93	5.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1778880.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.39352734876687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.05446938350508	18.796303062512507
2	6.9033071454553525	3.3679202329920583
3	3.044588680093366	2.2280477170081063
4	1.8262617330770583	1.7819586212728642
5	1.1837654301005607	1.4438107196841403
6	0.8513040151577934	1.2459784665520006
7	0.6440171082891843	1.0996894260888184
8	0.519925659324021	1.0146256632036923
9	0.4189963535674516	0.9198719108803102
>10	5.785645778031974	33.959583887033915
>50	1.6101880112958311	26.72455258920366
>100	0.15660131755031523	4.896929506135501
>500	4.646922760002436E-4	0.0799151603907773
>1k	2.323461380001218E-4	0.10133922466575164
>5k	0.0	0.0
>10k+	2.323461380001218E-4	2.3394738123759673
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41277	2.320392606583918	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1788	0.10051268213707501	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0238914373088685	0.0
2	0.0	0.0	0.0	0.07111216046051448	0.0
3	0.0	0.0	0.0	0.11153085087245908	0.0
4	0.0	0.0	0.0	0.1601569526893326	0.0
5	0.0	0.0	0.0	0.2716315884151826	0.0
6	0.0	0.0	0.0	0.45225085447022845	0.0
7	0.0	0.0	0.0	0.5663676020867062	0.0
8	0.0	0.0	0.0	0.7736890627810757	0.0
9	5.621514660910236E-5	0.0	0.0	0.8871312286382443	0.0
10	5.621514660910236E-5	0.0	0.0	1.047850332793668	0.0
11	5.621514660910236E-5	0.0	0.0	1.2683261377945674	0.0
12	5.621514660910236E-5	0.0	0.0	1.4248291059543083	0.0
13	5.621514660910236E-5	0.0	0.0	1.5098826227738802	0.0
14	5.621514660910236E-5	0.0	0.0	1.5601951789890267	0.0
15	5.621514660910236E-5	0.0	0.0	1.6122504047490556	0.0
16	5.621514660910236E-5	0.0	0.0	1.6967417701025365	0.0
17	5.621514660910236E-5	0.0	0.0	1.8065299514301134	0.0
18	5.621514660910236E-5	0.0	0.0	1.9273363014930742	0.0
19	5.621514660910236E-5	0.0	0.0	2.0137951969778736	0.0
20	5.621514660910236E-5	0.0	0.0	2.0940142111890627	0.0
21	5.621514660910236E-5	0.0	0.0	2.202172153264976	0.0
22	5.621514660910236E-5	0.0	0.0	2.328656233135456	0.0
23	5.621514660910236E-5	0.0	0.0	2.461998560892247	0.0
24	5.621514660910236E-5	0.0	0.0	2.5694819212088507	0.0
25	5.621514660910236E-5	0.0	0.0	2.6638109372189245	0.0
26	5.621514660910236E-5	0.0	0.0	2.7535865263536605	0.0
27	5.621514660910236E-5	0.0	0.0	2.8516819571865444	0.0
28	5.621514660910236E-5	0.0	0.0	2.9529816513761467	0.0
29	5.621514660910236E-5	0.0	0.0	3.0653557294477425	0.0
30	5.621514660910236E-5	5.621514660910236E-5	0.0	3.203588774959525	0.0
31	5.621514660910236E-5	5.621514660910236E-5	0.0	3.324957276488577	0.0
32	5.621514660910236E-5	5.621514660910236E-5	0.0	3.4453139053786654	0.0
33	5.621514660910236E-5	5.621514660910236E-5	0.0	3.5607236013671524	0.0
34	5.621514660910236E-5	5.621514660910236E-5	0.0	3.6843969239071774	0.0
35	5.621514660910236E-5	5.621514660910236E-5	0.0	3.8220116028062603	0.0
36	5.621514660910236E-5	5.621514660910236E-5	0.0	3.953779906457996	0.0
37	5.621514660910236E-5	5.621514660910236E-5	0.0	4.083693110271631	0.0
38	5.621514660910236E-5	5.621514660910236E-5	0.0	4.225017988846915	0.0
39	5.621514660910236E-5	5.621514660910236E-5	0.0	4.406761557834143	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCGA	20	7.0343306E-4	45.0	22
TCGCCGA	35	1.2124292E-7	45.0	28
AACCCGT	20	7.0343306E-4	45.0	31
GCGAACG	70	0.0	45.0	1
CGCGCGT	20	7.0343306E-4	45.0	45
AGACGCG	45	3.8562575E-10	45.0	1
ACGGCGC	25	3.8916703E-5	45.0	41
CGACTAT	20	7.0343306E-4	45.0	37
ATCGCCG	20	7.0343306E-4	45.0	1
CGTTTTT	17220	0.0	43.719513	1
TATTGCG	110	0.0	42.954544	1
CGACGGT	55	6.184564E-11	40.909092	28
CGACCCG	55	6.184564E-11	40.909092	32
TACGGGA	660	0.0	40.90909	4
AGGGCGA	1830	0.0	40.32787	6
GCGATCG	95	0.0	40.263157	9
TGGGCGA	805	0.0	39.40994	6
GCGATAT	160	0.0	39.375	9
TCGTCCC	400	0.0	38.8125	38
GGCACCG	825	0.0	38.727272	8
>>END_MODULE