FastQCFastQC Report
Thu 26 May 2016
SRR1547383_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547383_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1863320
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133310.7154434020994783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG551.8189894E-1245.01
TAATACG603.6379788E-1241.2500041
TACGATC501.0822987E-940.511
CGTTTTT72050.040.4718971
TATAGCG1450.040.3448261
GACCGAT10700.039.112159
CGTAAGG3650.038.8356172
GCGATAA1800.038.759
GGGCGAT34950.038.36917
ACGTAGG3300.038.181822
GCGTAAG1900.037.8947371
GTACGGG6550.037.786263
AGGGCGA19450.037.249366
TAAGGGA24750.037.181824
TTAGGGA23750.036.947374
GGCACCG10050.036.94038
TAGGGAC19950.036.6541375
CGTTAGG2950.036.610172
ATAGGGA24550.036.568234
CATACGA5550.036.4864918
Produced by FastQC (version 0.11.5)