##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547383_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1863320
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.303388575231306	31.0	31.0	33.0	30.0	34.0
2	31.717386707597193	31.0	31.0	34.0	30.0	34.0
3	31.83358467681343	31.0	31.0	34.0	30.0	34.0
4	35.620189768799776	37.0	35.0	37.0	33.0	37.0
5	35.527672648820385	37.0	35.0	37.0	33.0	37.0
6	35.55995159178241	37.0	35.0	37.0	33.0	37.0
7	35.905520790846445	37.0	35.0	37.0	35.0	37.0
8	35.8952858338879	37.0	35.0	37.0	35.0	37.0
9	37.65136530493957	39.0	37.0	39.0	35.0	39.0
10	36.92388263958955	39.0	37.0	39.0	32.0	39.0
11	36.6643373118949	39.0	35.0	39.0	32.0	39.0
12	36.2793658630831	38.0	35.0	39.0	32.0	39.0
13	36.12589624970483	38.0	35.0	39.0	31.0	39.0
14	37.191180795569196	39.0	35.0	41.0	32.0	41.0
15	37.33727164416203	39.0	35.0	41.0	32.0	41.0
16	37.419292982418476	39.0	35.0	41.0	32.0	41.0
17	37.322544705149944	39.0	35.0	41.0	32.0	41.0
18	37.19756778223815	39.0	35.0	41.0	32.0	41.0
19	37.156922053109504	39.0	35.0	41.0	32.0	41.0
20	37.0332814546079	39.0	35.0	41.0	32.0	41.0
21	36.904599854024	39.0	35.0	41.0	31.0	41.0
22	36.80277086061439	38.0	35.0	41.0	31.0	41.0
23	36.70359304896636	38.0	35.0	40.0	31.0	41.0
24	36.5874562608677	38.0	35.0	40.0	31.0	41.0
25	36.47352306635468	38.0	35.0	40.0	30.0	41.0
26	36.265350020393704	38.0	35.0	40.0	30.0	41.0
27	36.27477352252968	38.0	35.0	40.0	30.0	41.0
28	36.33093027499302	38.0	35.0	40.0	30.0	41.0
29	36.35891365948952	38.0	35.0	40.0	30.0	41.0
30	36.34619281712212	38.0	35.0	40.0	30.0	41.0
31	36.278456733142995	38.0	35.0	40.0	30.0	41.0
32	36.12846692999592	38.0	35.0	40.0	30.0	41.0
33	35.96744949874418	38.0	35.0	40.0	29.0	41.0
34	35.76734323680313	38.0	35.0	40.0	28.0	41.0
35	35.63616340725157	38.0	34.0	40.0	28.0	41.0
36	35.41748062597944	38.0	34.0	40.0	27.0	41.0
37	35.40021198720563	38.0	34.0	40.0	27.0	41.0
38	35.3102108065174	38.0	34.0	40.0	27.0	41.0
39	35.18056426164051	38.0	34.0	40.0	26.0	41.0
40	35.06473820921796	38.0	34.0	40.0	25.0	41.0
41	35.03343494407831	38.0	34.0	40.0	25.0	41.0
42	34.95130895391023	38.0	34.0	40.0	25.0	41.0
43	34.84439173088895	37.0	34.0	40.0	25.0	41.0
44	34.75305046905523	37.0	34.0	40.0	24.0	41.0
45	34.59339190262542	37.0	33.0	40.0	24.0	41.0
46	34.509990769164716	37.0	33.0	40.0	24.0	41.0
47	34.47130068909259	37.0	33.0	40.0	24.0	41.0
48	34.35697357405062	37.0	33.0	40.0	23.0	41.0
49	34.23165532490393	36.0	33.0	40.0	24.0	41.0
50	34.10591256466952	36.0	33.0	40.0	24.0	41.0
51	33.61235912242664	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	8.0
13	7.0
14	23.0
15	72.0
16	191.0
17	411.0
18	841.0
19	1684.0
20	2877.0
21	4484.0
22	6945.0
23	9834.0
24	14040.0
25	19864.0
26	27229.0
27	33152.0
28	37742.0
29	42789.0
30	49919.0
31	60230.0
32	73790.0
33	94475.0
34	143585.0
35	184330.0
36	153814.0
37	193958.0
38	278585.0
39	428320.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.76726488203851	23.920206942446814	28.59127793401026	14.72125024150441
2	31.23934697207136	26.03320953996093	29.300012880235276	13.427430607732434
3	28.59900607517764	25.93773479595561	31.776989459674134	13.686269669192624
4	24.966082047098727	28.308503101989995	31.778170147908035	14.947244703003241
5	22.976515037674687	32.94978854947084	29.97273683532619	14.100959577528283
6	22.402324882467852	41.36423158663032	26.40378464246614	9.829658888435695
7	90.54821501406093	2.580823476375502	5.821759010797931	1.049202498765644
8	91.12691325161539	2.0513384711160723	5.686838546250779	1.1349097310177532
9	86.19126076036322	4.449316274177275	7.173539703325248	2.1858832621342548
10	40.149840070411955	32.776764055556754	16.036000257604705	11.037395616426593
11	32.621181546916254	25.019213017624452	25.564637314041605	16.794968121417682
12	31.019846295859004	22.505635102934544	27.91195285833888	18.56256574286757
13	24.205128480346907	27.3121632355151	29.206309168580812	19.276399115557176
14	21.081403086963054	28.634534057488782	30.034561964665222	20.249500890882942
15	20.874406972500697	25.878539381319364	34.70906768563639	18.537985960543544
16	24.101066912822276	24.32013824785866	33.43204602537406	18.146748813945003
17	24.178294656849065	23.602816478114335	28.799508404353517	23.41938046068308
18	24.124358671618403	24.73520382972329	30.807215078462104	20.333222420196208
19	26.531084301139902	25.877519696026447	27.402861558937808	20.188534443895843
20	28.389594916600476	25.896088701886953	27.438550544189937	18.275765837322627
21	26.61845523044888	26.182566601549922	28.76183371616255	18.437144451838652
22	25.95388875770131	23.70634136916901	29.01476933645321	21.32500053667647
23	24.003338127643133	26.34061782195222	28.588111542837513	21.06793250756714
24	23.077034540497607	25.059356417577227	32.374471373677096	19.489137668248073
25	22.599607152823992	26.4714058776807	29.946010347122343	20.982976622372966
26	22.047850074061355	29.08883068930726	27.845029302535256	21.018289934096128
27	21.249651160294526	28.511313139986687	29.210548912693472	21.02848678702531
28	20.364403323100703	27.703400382113646	31.67840199214306	20.253794302642593
29	21.43754159242642	25.422203378915054	31.62972543631797	21.510529592340554
30	21.954414700641863	26.83774123607325	30.450325225940794	20.757518837344097
31	25.04647618229826	26.102065131056396	28.157106669815168	20.694352016830177
32	26.102387136938365	25.894532340124083	28.107839769873127	19.89524075306442
33	25.03928471760084	26.553946718760063	27.845619646652214	20.561148916986884
34	22.391430350127727	26.648187106884485	29.324431659618316	21.63595088336947
35	22.58983964107078	27.132215615138573	29.53545284760535	20.742491896185303
36	24.77749393555589	27.75272094970268	27.14289547689071	20.326889637850716
37	23.174011978618807	28.981012386492928	27.48518772942919	20.359787905459072
38	22.713865573277804	29.286327630251378	26.84241032136187	21.157396475108943
39	23.00608591116931	28.506751389992058	27.494203894124464	20.992958804714164
40	24.23545070089947	26.72863490973102	27.38359487366636	21.65231951570315
41	21.149346328059593	26.748867612648393	28.32229568726789	23.779490372024128
42	22.16940729450658	26.867097438979886	27.303576411995795	23.65991885451774
43	22.324560461971103	26.66493141274714	27.69052014683468	23.319987978447074
44	21.89076487130498	27.534776635253206	28.29793057553185	22.276527917909966
45	21.286842839662537	29.030386621728958	27.178155120966874	22.50461541764163
46	22.26021295322328	28.85602043663998	27.319623038447503	21.564143571689243
47	22.202788570937894	27.01978189468261	28.77229890732671	22.00513062705279
48	22.65225511452676	26.24176201618616	29.79778030612026	21.30820256316682
49	22.695833243887257	26.302406457291287	29.019760427623808	21.981999871197647
50	21.38977738660026	28.459899534164823	28.55854066934289	21.591782409892023
51	21.23483888972372	28.403602172466353	27.769035914389367	22.59252302342056
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1023.0
1	1722.5
2	2422.0
3	12210.5
4	21999.0
5	14982.0
6	7965.0
7	7942.5
8	7920.0
9	8393.5
10	8867.0
11	9132.5
12	9398.0
13	9241.0
14	9084.0
15	8717.0
16	8350.0
17	7920.5
18	7491.0
19	7962.0
20	8433.0
21	8144.0
22	7855.0
23	8965.5
24	10076.0
25	11393.0
26	15256.5
27	17803.0
28	22211.0
29	26619.0
30	31321.0
31	36023.0
32	41111.5
33	46200.0
34	51428.5
35	56657.0
36	60522.5
37	64388.0
38	71085.0
39	77782.0
40	91579.5
41	105377.0
42	115636.0
43	125895.0
44	129175.0
45	132455.0
46	131888.0
47	131321.0
48	130708.0
49	130095.0
50	126320.5
51	122546.0
52	117296.0
53	112046.0
54	107847.5
55	103649.0
56	98918.0
57	94187.0
58	89977.5
59	85768.0
60	79401.0
61	73034.0
62	66668.0
63	60302.0
64	52366.0
65	44430.0
66	38177.5
67	31925.0
68	26757.5
69	21590.0
70	18519.5
71	15449.0
72	12801.0
73	10153.0
74	8332.0
75	5206.5
76	3902.0
77	2900.0
78	1898.0
79	1414.5
80	931.0
81	695.5
82	460.0
83	318.5
84	177.0
85	128.0
86	79.0
87	53.5
88	28.0
89	22.5
90	17.0
91	16.5
92	16.0
93	14.0
94	12.0
95	7.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1863320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.32565882033604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.08349613693444	16.43856339294955
2	5.114872199648292	2.181560388786424
3	1.9524041778882955	1.2490891613113337
4	1.2055051795592429	1.0283276866171343
5	0.9074625833362774	0.9676118722225107
6	0.7164128360985392	0.9166785430288062
7	0.6314618318460639	0.9426437708809496
8	0.5696247436929885	0.971809835169443
9	0.5077438536386849	0.974517497173911
>10	9.751493770270535	51.871556636006666
>50	1.5186324527747792	20.431434012997833
>100	0.03987432801209884	1.165353547977941
>500	5.079531499069652E-4	0.06949007419201253
>1k	2.539765749534826E-4	0.06932758765843806
>5k	0.0	0.0
>10k+	2.539765749534826E-4	0.7220359930270608
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13331	0.7154434020994783	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.366764699568512E-5	0.0	0.0	0.015134276452783204	0.0
2	5.366764699568512E-5	0.0	0.0	0.04159242642165597	0.0
3	5.366764699568512E-5	0.0	0.0	0.0641865058068394	0.0
4	5.366764699568512E-5	0.0	0.0	0.1110920292810682	0.0
5	5.366764699568512E-5	0.0	0.0	0.18923212330678574	0.0
6	5.366764699568512E-5	0.0	0.0	0.3429899319494236	0.0
7	5.366764699568512E-5	0.0	0.0	0.4151729171586201	0.0
8	5.366764699568512E-5	0.0	0.0	0.5695210699182105	0.0
9	5.366764699568512E-5	0.0	0.0	0.6453534551231136	0.0
10	5.366764699568512E-5	0.0	0.0	0.7588605285189877	0.0
11	5.366764699568512E-5	0.0	0.0	0.8822424489620677	0.0
12	5.366764699568512E-5	0.0	0.0	0.9835132988429255	0.0
13	5.366764699568512E-5	0.0	0.0	1.0387373076014854	0.0
14	5.366764699568512E-5	0.0	0.0	1.07276259579675	0.0
15	5.366764699568512E-5	0.0	0.0	1.1050168516411567	0.0
16	5.366764699568512E-5	0.0	0.0	1.1595968486357684	0.0
17	5.366764699568512E-5	0.0	0.0	1.2220123220917503	0.0
18	5.366764699568512E-5	0.0	0.0	1.2956443337698302	0.0
19	5.366764699568512E-5	0.0	0.0	1.3472726101796793	0.0
20	5.366764699568512E-5	0.0	0.0	1.3998669042354508	0.0
21	5.366764699568512E-5	0.0	0.0	1.4706545306227594	0.0
22	5.366764699568512E-5	0.0	0.0	1.554054054054054	0.0
23	5.366764699568512E-5	0.0	0.0	1.6424446686559475	0.0
24	5.366764699568512E-5	0.0	0.0	1.7144666509241568	0.0
25	5.366764699568512E-5	0.0	0.0	1.778706824377992	0.0
26	5.366764699568512E-5	0.0	0.0	1.8423566537148746	0.0
27	5.366764699568512E-5	0.0	0.0	1.9068651654036881	0.0
28	5.366764699568512E-5	0.0	0.0	1.976257432969109	0.0
29	5.366764699568512E-5	0.0	0.0	2.052250821114999	0.0
30	5.366764699568512E-5	0.0	0.0	2.1443981710065905	0.0
31	5.366764699568512E-5	0.0	0.0	2.2299444003177125	0.0
32	5.366764699568512E-5	0.0	0.0	2.3154369619818387	0.0
33	5.366764699568512E-5	0.0	0.0	2.3979241354142067	0.0
34	5.366764699568512E-5	0.0	0.0	2.4823970117854155	0.0
35	5.366764699568512E-5	0.0	0.0	2.5825408410793638	0.0
36	5.366764699568512E-5	0.0	0.0	2.676405555674817	0.0
37	5.366764699568512E-5	0.0	0.0	2.7777837408496664	0.0
38	5.366764699568512E-5	0.0	0.0	2.8949938818882424	0.0
39	5.366764699568512E-5	0.0	0.0	3.0635639611016896	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCG	55	1.8189894E-12	45.0	1
TAATACG	60	3.6379788E-12	41.250004	1
TACGATC	50	1.0822987E-9	40.5	11
CGTTTTT	7205	0.0	40.471897	1
TATAGCG	145	0.0	40.344826	1
GACCGAT	1070	0.0	39.11215	9
CGTAAGG	365	0.0	38.835617	2
GCGATAA	180	0.0	38.75	9
GGGCGAT	3495	0.0	38.3691	7
ACGTAGG	330	0.0	38.18182	2
GCGTAAG	190	0.0	37.894737	1
GTACGGG	655	0.0	37.78626	3
AGGGCGA	1945	0.0	37.24936	6
TAAGGGA	2475	0.0	37.18182	4
TTAGGGA	2375	0.0	36.94737	4
GGCACCG	1005	0.0	36.9403	8
TAGGGAC	1995	0.0	36.654137	5
CGTTAGG	295	0.0	36.61017	2
ATAGGGA	2455	0.0	36.56823	4
CATACGA	555	0.0	36.48649	18
>>END_MODULE