FastQCFastQC Report
Thu 26 May 2016
SRR1547382_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547382_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3037399
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384731.2666429402261605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT182200.043.172341
CGTAAGG5850.042.692312
CGTTAGG5600.039.7767872
TCGTTAG1250.039.61
AGGGCGA24750.038.9090926
GCGTAAG3200.038.6718751
GGGCGAT51350.038.5150957
TACGGGA11850.037.784814
TGGGCGA16100.037.7329186
ATAGGGA50400.037.6785744
TAAGGGA46850.037.5080034
AGGGATC47100.037.4522326
TAGGGAT61900.037.330375
TCGTAAG1450.037.2413831
AGGGATT66350.037.2004556
CGAGGGA23900.037.186194
ATAGGGC11650.037.0815474
GCGATCG2550.037.0588239
TAGGGAC41150.036.6342665
AAGGGAT55400.036.592965
Produced by FastQC (version 0.11.5)