FastQCFastQC Report
Thu 26 May 2016
SRR1547381_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547381_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3762388
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT542831.442780489412575No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT274950.043.0932921
GTCGATA900.040.025
TAGGGTA26100.039.741385
CGACGGT1200.039.37500428
AGGGTAC20000.038.256
AGGGCGA30500.038.2131166
GGGCGAT62100.038.0434767
TAAGGGT25000.037.984
GACCGAT24350.037.885019
TAGGGTG28200.037.819155
TGCGTAG2450.037.653061
GGCGATA12550.037.470128
ATGGGAC22550.037.416855
CGTAAGG6100.037.25412
ATATACG1150.037.1739121
GGGACCG27750.037.1351367
TAGGGCG13950.037.0967755
ACGGGTA5650.037.03545
GTTTTTT326650.036.961582
CGATCGA552.752131E-936.8181810
Produced by FastQC (version 0.11.5)