##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547381_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3762388
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.277074028515933	31.0	31.0	33.0	30.0	34.0
2	31.681999836274198	31.0	31.0	34.0	30.0	34.0
3	31.716834095792354	31.0	31.0	34.0	30.0	34.0
4	35.53497805117388	37.0	35.0	37.0	33.0	37.0
5	35.451684940521815	37.0	35.0	37.0	33.0	37.0
6	35.49305733486286	37.0	35.0	37.0	33.0	37.0
7	35.92562622462117	37.0	35.0	37.0	35.0	37.0
8	35.92906978227658	37.0	35.0	37.0	35.0	37.0
9	37.681150375771985	39.0	37.0	39.0	35.0	39.0
10	36.813251849623164	39.0	37.0	39.0	32.0	39.0
11	36.60712425193786	39.0	35.0	39.0	32.0	39.0
12	36.20875624736205	38.0	35.0	39.0	32.0	39.0
13	35.996600829047935	38.0	35.0	39.0	31.0	39.0
14	37.06180755413849	39.0	35.0	41.0	31.0	41.0
15	37.248101737513515	39.0	35.0	41.0	32.0	41.0
16	37.351054968280785	39.0	35.0	41.0	32.0	41.0
17	37.2966456410131	39.0	35.0	41.0	32.0	41.0
18	37.12203393164129	39.0	35.0	41.0	32.0	41.0
19	37.003132584943394	38.0	35.0	41.0	32.0	41.0
20	36.795525873461216	38.0	35.0	40.0	31.0	41.0
21	36.68223027502746	38.0	35.0	40.0	31.0	41.0
22	36.56921960201872	38.0	35.0	40.0	31.0	41.0
23	36.48044566376461	38.0	35.0	40.0	31.0	41.0
24	36.36881204171393	38.0	35.0	40.0	31.0	41.0
25	36.26598080793369	38.0	35.0	40.0	30.0	41.0
26	36.05266229851892	38.0	35.0	40.0	30.0	41.0
27	36.02570495121715	38.0	35.0	40.0	30.0	41.0
28	36.0659102676279	38.0	35.0	40.0	30.0	41.0
29	36.07540264321489	38.0	35.0	40.0	30.0	41.0
30	36.03706422623079	38.0	35.0	40.0	30.0	41.0
31	35.917486447437106	38.0	35.0	40.0	30.0	41.0
32	35.69457748642618	38.0	34.0	40.0	29.0	41.0
33	35.422819496553785	38.0	34.0	40.0	27.0	41.0
34	35.14440801958756	38.0	34.0	40.0	25.0	41.0
35	34.942244925297445	38.0	34.0	40.0	24.0	41.0
36	34.67955351760637	38.0	34.0	40.0	23.0	41.0
37	34.617551671970034	38.0	34.0	40.0	23.0	41.0
38	34.54533291090658	37.0	33.0	40.0	23.0	41.0
39	34.419261649782	37.0	33.0	40.0	23.0	41.0
40	34.31650776049679	37.0	33.0	40.0	23.0	41.0
41	34.30925066739528	37.0	33.0	40.0	23.0	41.0
42	34.211841521927035	37.0	33.0	40.0	22.0	41.0
43	34.09879922006981	37.0	33.0	40.0	22.0	41.0
44	34.00545743820148	37.0	33.0	40.0	21.0	41.0
45	33.852410490358785	36.0	33.0	40.0	21.0	41.0
46	33.75894963517851	36.0	33.0	40.0	20.0	41.0
47	33.73226259492641	36.0	33.0	40.0	20.0	41.0
48	33.629258066951095	36.0	33.0	40.0	20.0	41.0
49	33.51593137124613	36.0	33.0	40.0	20.0	41.0
50	33.3905984709711	36.0	32.0	40.0	20.0	41.0
51	32.91630554849739	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	9.0
13	16.0
14	45.0
15	128.0
16	398.0
17	887.0
18	2205.0
19	4140.0
20	7135.0
21	11234.0
22	16925.0
23	25249.0
24	37055.0
25	54215.0
26	74444.0
27	85662.0
28	89766.0
29	95152.0
30	106662.0
31	125028.0
32	150767.0
33	190465.0
34	293454.0
35	398592.0
36	301745.0
37	360784.0
38	518892.0
39	811116.0
40	214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.451720556199945	25.013528641915723	28.512636123653383	17.022114678230952
2	33.25055789036112	24.86840804297696	29.125890259058874	12.755143807603044
3	27.13263491165717	24.685359404718493	35.0796887508678	13.102316932756535
4	23.86681012165678	27.584183236816617	34.82128903239113	13.727717609135476
5	22.176341196070158	31.931608329603435	32.91337841817484	12.978672056151572
6	20.8966486178459	40.5253259366126	29.29455973174484	9.283465713796662
7	87.22186547479951	2.670245599337442	9.064881134003192	1.0430077918598508
8	87.53254581930412	2.2989654442869796	8.933209440387328	1.2352792960215693
9	83.20069594098216	4.15948594350184	10.667241124519853	1.972576990996144
10	37.97000734639809	24.694183587657626	24.367954607552438	12.96785445839185
11	32.77551384918302	23.584409688740234	25.980467724221956	17.659608737854786
12	30.867815865880928	22.885119769678195	28.430906116009304	17.81615824843158
13	22.616965608012784	29.35159797447791	30.078397018064056	17.953039399445245
14	19.25984241922949	31.48394051862806	31.300599512862576	17.95561754927987
15	18.720078843542982	25.665455024840604	38.945823769372005	16.668642362244405
16	20.188348463794803	23.663109705857025	38.350722998265994	17.797818832082175
17	20.684310071156936	23.814449759036016	31.164887831876985	24.33635233793006
18	21.68226668807151	25.38898167865728	34.292077265821604	18.63667436744961
19	24.795103535307895	26.60531024445113	29.872012136972582	18.72757408326839
20	26.202348083185466	26.20979016518233	30.261791181558095	17.326070570074112
21	23.35216357270967	27.538441011400206	31.40975890843794	17.699636507452183
22	23.429242279105715	24.647803469498626	30.798391872395936	21.12456237899972
23	20.837005646413925	28.452169207428902	30.154013886925007	20.55681125923217
24	20.983056505602292	26.170719234698815	35.37862123736308	17.467603022335815
25	19.981086480182267	27.349252655494325	33.39145244988024	19.278208414443167
26	19.451635503834268	30.929930671690425	30.17094462346786	19.447489201007446
27	19.145898828084718	30.637137902842554	31.36377747324306	18.85318579582967
28	17.845049473897962	28.63327758859533	35.24532823302648	18.27634470448024
29	18.673592409926886	26.704024146366617	34.722734603661294	19.899648840045206
30	19.86445842374577	29.489356228012635	32.420579695661374	18.225605652580224
31	23.73458027189115	27.438212114221074	29.87286266062937	18.95434495325841
32	24.317853448395006	27.379260193260237	30.195264284279027	18.107622074065727
33	23.443648023542497	28.810478876713407	28.97282257970204	18.77305052004206
34	20.13532362956718	28.689943727228556	30.396413129108428	20.778319514095838
35	20.165543798247285	28.778318450941264	30.917730972988434	20.138406777823022
36	24.029579086473802	28.808857565992664	28.009179276565842	19.152384070967692
37	20.99945566485966	30.928734622798075	29.538128443956342	18.53368126838593
38	21.03379555750231	31.103065393574507	27.735310659081414	20.12782838984177
39	20.357895039001825	31.122228754716417	28.50859613628366	20.011280069998097
40	22.388095007745083	28.72050942114423	28.030442367985437	20.86095320312525
41	18.770472370207433	28.30061652333571	29.315370982471773	23.613540123985086
42	20.627298407288137	28.851197696781938	27.849147934769086	22.672355961160836
43	20.72808546061703	28.24240881057456	29.121292115539386	21.90821361326902
44	20.404886471039138	29.929635114719694	28.831316706304612	20.834161707936556
45	19.61318184089467	32.33241760286286	27.218750431906546	20.835650124335928
46	21.20924264057827	31.505868081654526	27.55736516276365	19.727524115003554
47	20.767103233371998	29.01324903226355	28.895265453749055	21.3243822806154
48	20.994458838376055	28.092955856759062	30.577760720053327	20.33482458481156
49	21.091019852285303	27.28248654843679	30.401755480827603	21.224738118450304
50	19.700094727072273	30.530955340065937	29.26146904572309	20.5074808871387
51	19.403527759497425	31.139823962866135	27.64122147954969	21.815426798086747
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1888.0
1	3106.0
2	4324.0
3	41609.0
4	78894.0
5	53003.5
6	27113.0
7	27451.0
8	27789.0
9	29940.5
10	32092.0
11	34061.0
12	36030.0
13	35720.0
14	35410.0
15	33242.0
16	31074.0
17	29140.0
18	27206.0
19	26233.5
20	25261.0
21	25857.0
22	26453.0
23	25748.5
24	25044.0
25	28965.5
26	37079.5
27	41272.0
28	49361.0
29	57450.0
30	65210.0
31	72970.0
32	82810.0
33	92650.0
34	106151.5
35	119653.0
36	125084.5
37	130516.0
38	145617.5
39	160719.0
40	192043.5
41	223368.0
42	250723.0
43	278078.0
44	286055.0
45	294032.0
46	291368.0
47	288704.0
48	282759.0
49	276814.0
50	268406.5
51	259999.0
52	246779.5
53	233560.0
54	212072.5
55	190585.0
56	177557.0
57	164529.0
58	150817.0
59	137105.0
60	121366.5
61	105628.0
62	92654.5
63	79681.0
64	66787.5
65	53894.0
66	43558.0
67	33222.0
68	26914.0
69	20606.0
70	16836.5
71	13067.0
72	11159.0
73	9251.0
74	7722.0
75	4943.0
76	3693.0
77	2847.5
78	2002.0
79	1519.0
80	1036.0
81	685.5
82	335.0
83	233.5
84	132.0
85	122.0
86	112.0
87	72.0
88	32.0
89	27.0
90	22.0
91	13.0
92	4.0
93	5.5
94	7.0
95	4.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3762388.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.27310837493886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.74671955302547	17.17733715972306
2	7.23188796768885	3.0768947298412255
3	2.47641796983866	1.580433235620717
4	1.3147500042498061	1.1187527730542983
5	0.8856596577469903	0.9420366941281493
6	0.5879279481800664	0.7504232974973996
7	0.4517959312040849	0.6727772665502644
8	0.35289051287435	0.6005662499891046
9	0.30202098526955956	0.5782432636030639
>10	3.3280746310556504	16.94186091387092
>50	1.2566507070783208	19.308614014571233
>100	1.060153910595977	34.97591202116639
>500	0.00454519907298536	0.6616854069707958
>1k	3.787665894154466E-4	0.1565043378153445
>5k	0.0	0.0
>10k+	1.2625552980514887E-4	1.4579586355981373
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54283	1.442780489412575	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6578864274498007E-5	0.0	0.0	0.029582275937516278	0.0
2	2.6578864274498007E-5	0.0	0.0	0.08683314958478498	0.0
3	2.6578864274498007E-5	0.0	0.0	0.13624325827107678	0.0
4	2.6578864274498007E-5	0.0	0.0	0.2212956239494704	0.0
5	2.6578864274498007E-5	0.0	0.0	0.3746822496775984	0.0
6	2.6578864274498007E-5	0.0	0.0	0.6600595153928834	0.0
7	2.6578864274498007E-5	0.0	0.0	0.8124361442785805	0.0
8	2.6578864274498007E-5	0.0	0.0	1.1297877837160868	0.0
9	2.6578864274498007E-5	0.0	0.0	1.26541972810885	0.0
10	2.6578864274498007E-5	0.0	0.0	1.4959382179615712	0.0
11	2.6578864274498007E-5	0.0	0.0	1.7677868417611369	0.0
12	2.6578864274498007E-5	0.0	0.0	1.9747564578666528	0.0
13	2.6578864274498007E-5	0.0	0.0	2.094547399151815	0.0
14	2.6578864274498007E-5	0.0	0.0	2.1533664257912792	0.0
15	2.6578864274498007E-5	0.0	0.0	2.2206375312700337	0.0
16	2.6578864274498007E-5	0.0	0.0	2.3484552895660946	0.0
17	2.6578864274498007E-5	0.0	0.0	2.495489566732618	0.0
18	2.6578864274498007E-5	0.0	0.0	2.652969337559018	0.0
19	2.6578864274498007E-5	0.0	0.0	2.754101916123483	0.0
20	2.6578864274498007E-5	0.0	0.0	2.859832638207436	0.0
21	2.6578864274498007E-5	0.0	0.0	3.0096842749870563	0.0
22	2.6578864274498007E-5	0.0	0.0	3.168333515841535	0.0
23	2.6578864274498007E-5	0.0	0.0	3.3471561146803572	0.0
24	2.6578864274498007E-5	0.0	0.0	3.4865091000715505	0.0
25	5.315772854899601E-5	0.0	0.0	3.6136889656250233	0.0
26	5.315772854899601E-5	0.0	0.0	3.7352341119523027	0.0
27	5.315772854899601E-5	0.0	0.0	3.8563805753154647	0.0
28	5.315772854899601E-5	0.0	0.0	3.991640415608385	0.0
29	5.315772854899601E-5	0.0	0.0	4.129372090278833	0.0
30	5.315772854899601E-5	0.0	0.0	4.290652638696487	0.0
31	5.315772854899601E-5	0.0	0.0	4.432982456886425	0.0
32	5.315772854899601E-5	0.0	0.0	4.573079650477303	0.0
33	5.315772854899601E-5	0.0	0.0	4.718120512823239	0.0
34	5.315772854899601E-5	0.0	0.0	4.863746110183214	0.0
35	5.315772854899601E-5	0.0	0.0	5.031060060791178	0.0
36	5.315772854899601E-5	0.0	0.0	5.185669314275933	0.0
37	5.315772854899601E-5	0.0	0.0	5.3555082569899755	0.0
38	5.315772854899601E-5	0.0	0.0	5.528403769095585	0.0
39	5.315772854899601E-5	0.0	0.0	5.740051265313412	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	27495	0.0	43.093292	1
GTCGATA	90	0.0	40.0	25
TAGGGTA	2610	0.0	39.74138	5
CGACGGT	120	0.0	39.375004	28
AGGGTAC	2000	0.0	38.25	6
AGGGCGA	3050	0.0	38.213116	6
GGGCGAT	6210	0.0	38.043476	7
TAAGGGT	2500	0.0	37.98	4
GACCGAT	2435	0.0	37.88501	9
TAGGGTG	2820	0.0	37.81915	5
TGCGTAG	245	0.0	37.65306	1
GGCGATA	1255	0.0	37.47012	8
ATGGGAC	2255	0.0	37.41685	5
CGTAAGG	610	0.0	37.2541	2
ATATACG	115	0.0	37.173912	1
GGGACCG	2775	0.0	37.135136	7
TAGGGCG	1395	0.0	37.096775	5
ACGGGTA	565	0.0	37.0354	5
GTTTTTT	32665	0.0	36.96158	2
CGATCGA	55	2.752131E-9	36.81818	10
>>END_MODULE