##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547379_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1861892
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210129266359164	31.0	31.0	33.0	30.0	34.0
2	31.579241438278913	31.0	31.0	34.0	30.0	34.0
3	31.48700998768994	31.0	31.0	34.0	30.0	34.0
4	35.35571236140442	37.0	35.0	37.0	33.0	37.0
5	35.29388063324833	37.0	35.0	37.0	33.0	37.0
6	35.35062076640321	37.0	35.0	37.0	33.0	37.0
7	35.865020097835966	37.0	35.0	37.0	35.0	37.0
8	35.88471135812389	37.0	35.0	37.0	35.0	37.0
9	37.63456312181373	39.0	37.0	39.0	35.0	39.0
10	36.83306281997022	39.0	37.0	39.0	32.0	39.0
11	36.52199321980007	39.0	35.0	39.0	32.0	39.0
12	35.895080380602096	37.0	35.0	39.0	31.0	39.0
13	35.528847000792744	37.0	35.0	39.0	30.0	39.0
14	36.63071542280648	39.0	35.0	41.0	31.0	41.0
15	36.90905433827526	39.0	35.0	41.0	31.0	41.0
16	37.0978461693804	39.0	35.0	41.0	32.0	41.0
17	37.04779654244177	38.0	35.0	41.0	32.0	41.0
18	36.82817155882296	38.0	35.0	40.0	32.0	41.0
19	36.608719517566	37.0	35.0	40.0	31.0	41.0
20	36.29334300807995	37.0	35.0	40.0	31.0	41.0
21	36.1269504353636	37.0	35.0	40.0	31.0	41.0
22	36.012925561740424	37.0	35.0	40.0	30.0	41.0
23	35.93815430755382	36.0	34.0	40.0	30.0	41.0
24	35.750499491914674	36.0	34.0	40.0	30.0	41.0
25	35.62172671669463	36.0	34.0	40.0	30.0	41.0
26	35.36339540639307	35.0	34.0	40.0	30.0	41.0
27	35.25295559570587	35.0	34.0	40.0	29.0	41.0
28	35.182243116142075	36.0	34.0	40.0	29.0	41.0
29	35.14939051244648	36.0	34.0	40.0	29.0	41.0
30	35.06106691472975	36.0	34.0	40.0	29.0	41.0
31	34.85183082584812	35.0	34.0	40.0	27.0	41.0
32	34.50032762372898	35.0	33.0	40.0	25.0	41.0
33	34.09609526223863	35.0	33.0	40.0	23.0	41.0
34	33.69752434620268	35.0	33.0	40.0	21.0	41.0
35	33.39047270196123	35.0	33.0	40.0	19.0	41.0
36	33.00127934380727	35.0	33.0	40.0	18.0	41.0
37	32.89950920891223	35.0	32.0	40.0	18.0	41.0
38	32.84650989423662	35.0	32.0	40.0	17.0	41.0
39	32.734285339858594	35.0	32.0	40.0	16.0	41.0
40	32.623550667815316	35.0	32.0	40.0	15.0	41.0
41	32.6208689870304	35.0	32.0	40.0	15.0	41.0
42	32.51315489835071	35.0	32.0	39.0	15.0	41.0
43	32.38732858833917	35.0	31.0	39.0	15.0	41.0
44	32.28061670601732	35.0	31.0	39.0	15.0	41.0
45	32.14410234320788	35.0	31.0	39.0	14.0	41.0
46	32.06473468923009	35.0	31.0	39.0	13.0	41.0
47	32.03609715278867	35.0	31.0	39.0	13.0	41.0
48	31.945002717665687	35.0	31.0	39.0	12.0	41.0
49	31.829010490404386	35.0	31.0	39.0	10.0	41.0
50	31.67171726394442	35.0	31.0	39.0	10.0	41.0
51	31.263838611476928	35.0	30.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	8.0
13	21.0
14	38.0
15	106.0
16	284.0
17	745.0
18	1624.0
19	3201.0
20	5390.0
21	8498.0
22	12548.0
23	18748.0
24	27600.0
25	40032.0
26	53904.0
27	56977.0
28	53779.0
29	53910.0
30	58480.0
31	67660.0
32	81674.0
33	103331.0
34	164034.0
35	248255.0
36	174002.0
37	150370.0
38	188521.0
39	288048.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.207328889108496	23.19124847198441	27.40040775727056	21.201014881636528
2	36.803423614259046	24.4573262036681	26.923151289118813	11.816098892954049
3	24.77533605601184	23.650781033486368	39.29997013790273	12.273912772599054
4	21.437494763391218	26.11907672410645	39.2935787897472	13.149849722755134
5	19.961791553967682	30.170278404977303	37.30382857867159	12.564101462383427
6	18.36314888296421	38.4052888137443	34.17754628087988	9.054016022411611
7	80.71757115879976	3.172901543161472	14.725558732729931	1.3839685653088363
8	81.10078350409154	2.86627795812002	14.330154488015417	1.7027840497730267
9	77.51717070592709	4.688295561718939	15.810852616585711	1.9836811157682617
10	42.237304849046026	23.054183593892663	23.50163167358794	11.20687988347337
11	34.55882510908259	25.226651169885255	27.907741157918935	12.30678256311322
12	32.678103778307225	23.81212229280753	30.38484509305588	13.12492883582936
13	17.61922818294509	40.59360048810564	29.307714948020614	12.479456380928648
14	13.967458907390975	40.778358787727754	31.07887031041543	14.175311994465844
15	12.170630734757976	30.414868316744474	44.913131373892796	12.501369574604757
16	12.7571309184421	26.806173505230163	44.93864305770689	15.498052518620845
17	13.388853918487218	28.512824589181328	32.19402629153571	25.90429520079575
18	16.445475892264426	30.255514283320405	37.0664356471804	16.23257417723477
19	21.493137088509968	33.484541530872896	28.73120460263001	16.29111677798712
20	23.597072225456685	29.810536808794495	31.205569388557446	15.386821577191373
21	16.852857201169563	35.264666264208664	31.50392181716233	16.378554717459444
22	18.89137500993613	31.760596210736175	28.89533871996872	20.452690059358975
23	15.907206218190959	36.37112141842814	28.918111254573304	18.8035611088076
24	15.23547015616373	31.237633546951166	40.16817301970254	13.358723277182566
25	13.36645734553884	34.44958139355022	36.04467928322373	16.13928197768721
26	14.449012080185103	39.35029529102655	30.53549829957914	15.665194329209212
27	14.093406062220579	38.98819050729043	31.023550238144853	15.894853192344133
28	12.700575543586845	35.30118825366885	37.46726448150591	14.530971721238394
29	12.48241036537028	31.36132493184352	37.15430325711696	19.001961445669245
30	14.324085392708064	38.01471836175245	33.00325690211892	14.657939343420564
31	20.38104250944738	34.968032517460735	28.504284888704607	16.14664008438728
32	21.803949960577736	33.48427298683275	28.941044915601978	15.770732136987537
33	19.72101496757062	35.25279661763411	27.975199420804213	17.05098899399106
34	14.351638011227289	34.59905300629682	29.673579348318807	21.375729634157086
35	15.055223396416118	33.972808304670735	32.031127476781684	18.940840822131467
36	22.423427352392082	34.47885269392639	26.685973192859734	16.41174676082179
37	15.242935680479855	38.271231628902214	31.062650250390462	15.423182440227468
38	15.244546944720746	38.69434961856005	29.091966666165387	16.969136770553824
39	15.371568275710942	40.354811127605686	28.244065713800797	16.029554882882575
40	19.71306606398223	35.20402901994316	27.097812332831335	17.985092583243283
41	14.414638443046105	33.036556363097326	29.76891248257149	22.77989271128508
42	16.863115583503234	34.62537032223136	26.704502731629976	21.807011362635425
43	16.914783456827788	35.65964083845895	27.536183623969595	19.889392080743672
44	15.8781497530469	37.812236155480555	27.76482201975195	18.544792071720593
45	14.169296607966519	42.412019601566584	23.918465732706302	19.500218057760602
46	17.22634825220797	40.31807430291338	25.62039044155085	16.835187003327796
47	16.49037645577724	35.90987017506923	28.295733587125355	19.304019782028174
48	17.527010159558127	33.02468671652276	30.787124065198196	18.661179058720915
49	16.46835584448507	33.44350800153822	30.0678557080647	20.020280445912007
50	15.67770848148013	38.16236387502605	28.702148137485956	17.457779506007867
51	13.878248577253673	38.94173238834476	26.79806347521768	20.381955559183883
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1619.0
1	1962.5
2	2306.0
3	37595.5
4	72885.0
5	45928.5
6	18972.0
7	21744.5
8	24517.0
9	30686.5
10	36856.0
11	42225.5
12	47595.0
13	48805.0
14	50015.0
15	45904.5
16	41794.0
17	37184.5
18	32575.0
19	29210.5
20	25846.0
21	24031.0
22	22216.0
23	20978.0
24	19740.0
25	19989.5
26	23021.0
27	25803.0
28	29178.5
29	32554.0
30	35743.0
31	38932.0
32	44378.0
33	49824.0
34	56027.0
35	62230.0
36	62255.0
37	62280.0
38	72406.0
39	82532.0
40	101425.0
41	120318.0
42	133893.0
43	147468.0
44	152454.0
45	157440.0
46	152086.0
47	146732.0
48	141388.0
49	136044.0
50	127381.5
51	118719.0
52	102910.0
53	87101.0
54	73929.0
55	60757.0
56	51011.5
57	41266.0
58	34798.5
59	28331.0
60	23297.0
61	18263.0
62	15143.5
63	12024.0
64	9488.0
65	6952.0
66	5307.0
67	3662.0
68	3044.5
69	2427.0
70	1692.5
71	958.0
72	798.0
73	638.0
74	560.0
75	434.5
76	387.0
77	324.0
78	261.0
79	218.5
80	176.0
81	132.5
82	89.0
83	62.0
84	35.0
85	20.5
86	6.0
87	8.5
88	11.0
89	7.5
90	4.0
91	5.0
92	6.0
93	5.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1861892.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.543968520674845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.63782956099718	22.446357315232973
2	7.340071296153685	4.190295280338395
3	3.2310498688469433	2.7668095723529325
4	2.0061863312352815	2.290580779415521
5	1.4353333711198797	2.0485055280959976
6	1.0104520994557975	1.7305387751109655
7	0.7087249541457609	1.4160875946667328
8	0.5444477430666546	1.2432559391397644
9	0.42852629582910184	1.100865698858455
>10	3.368615549706249	20.004020996925938
>50	0.7801954136420428	16.220932641075144
>100	0.5039944125048903	20.00660004851866
>500	0.0030487355310650594	0.5590684564639103
>1k	0.0013338217948409635	0.587514103193225
>5k	0.0	0.0
>10k+	1.9054597069156622E-4	3.3885672706113965
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62302	3.3461661578652255	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2740	0.1471621340013277	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2176	0.11687036627258723	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0741761605936328E-4	0.0	0.0	0.06778051573345822	0.0
2	1.0741761605936328E-4	0.0	0.0	0.18406008511771896	0.0
3	1.0741761605936328E-4	0.0	0.0	0.27455942664773253	0.0
4	1.0741761605936328E-4	0.0	0.0	0.43423571291997604	0.0
5	1.0741761605936328E-4	0.0	0.0	0.7657264760791711	0.0
6	1.0741761605936328E-4	0.0	0.0	1.4673783441789319	0.0
7	1.0741761605936328E-4	0.0	0.0	1.9470517086920187	0.0
8	1.0741761605936328E-4	0.0	0.0	2.6955913662016915	0.0
9	1.0741761605936328E-4	0.0	0.0	3.0861618181935366	0.0
10	1.0741761605936328E-4	0.0	0.0	3.5076685436104778	0.0
11	1.0741761605936328E-4	0.0	0.0	3.893297785263592	0.0
12	1.0741761605936328E-4	0.0	0.0	4.173926307218679	0.0
13	1.0741761605936328E-4	0.0	0.0	4.3440221022486805	0.0
14	1.0741761605936328E-4	0.0	0.0	4.425390946413648	0.0
15	1.0741761605936328E-4	0.0	0.0	4.502839047592449	0.0
16	1.0741761605936328E-4	0.0	0.0	4.649249258281361	0.0
17	1.0741761605936328E-4	0.0	0.0	4.8268642864355185	0.0
18	1.0741761605936328E-4	0.0	0.0	5.0374565227199	0.0
19	1.0741761605936328E-4	0.0	0.0	5.160503401915901	0.0
20	1.0741761605936328E-4	0.0	0.0	5.282690940183426	0.0
21	1.0741761605936328E-4	0.0	0.0	5.462132067810592	0.0
22	1.0741761605936328E-4	0.0	0.0	5.648447922865558	0.0
23	1.0741761605936328E-4	0.0	0.0	5.886270524820988	0.0
24	1.0741761605936328E-4	0.0	0.0	6.057709040051733	0.0
25	1.0741761605936328E-4	0.0	0.0	6.219104008180926	0.0
26	1.0741761605936328E-4	0.0	0.0	6.3613786406515525	0.0
27	1.0741761605936328E-4	0.0	0.0	6.502310552921437	0.0
28	1.0741761605936328E-4	0.0	0.0	6.661718295153532	0.0
29	1.0741761605936328E-4	0.0	0.0	6.829719446670376	0.0
30	1.0741761605936328E-4	0.0	0.0	7.05164424144902	0.0
31	1.0741761605936328E-4	0.0	0.0	7.2242643504564175	0.0
32	1.0741761605936328E-4	0.0	0.0	7.399945861521506	0.0
33	1.0741761605936328E-4	0.0	0.0	7.579924077228969	0.0
34	1.0741761605936328E-4	0.0	0.0	7.759096660815987	0.0
35	1.0741761605936328E-4	0.0	0.0	7.971246452533229	0.0
36	1.0741761605936328E-4	0.0	0.0	8.148163266183001	0.0
37	1.611264240890449E-4	0.0	0.0	8.34898049940598	0.0
38	1.611264240890449E-4	0.0	0.0	8.545662154410675	0.0
39	1.611264240890449E-4	0.0	0.0	8.758563869440334	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	30	2.166229E-6	45.000004	1
CCGGCTA	30	2.166229E-6	45.000004	34
CACGATG	30	2.166229E-6	45.000004	1
CTACGCG	30	2.166229E-6	45.000004	1
TAACGTT	20	7.034416E-4	45.0	22
GCGCATC	20	7.034416E-4	45.0	33
TCTAGCG	50	2.1827873E-11	45.0	1
TGTTCGA	20	7.034416E-4	45.0	20
ACGCTAG	20	7.034416E-4	45.0	1
TCCGTAG	25	3.891741E-5	45.0	1
ACCGTGC	20	7.034416E-4	45.0	31
CGGTCGT	25	3.891741E-5	45.0	36
GTCGGTC	25	3.891741E-5	45.0	34
AGTCGCG	20	7.034416E-4	45.0	1
AGGTCGC	20	7.034416E-4	45.0	42
CGTTTTT	22105	0.0	44.44017	1
TGTTGCG	95	0.0	42.63158	1
CGTTAGG	270	0.0	41.666664	2
ACGGGCC	120	0.0	41.250004	5
TACTCGC	60	3.6379788E-12	41.250004	45
>>END_MODULE