FastQCFastQC Report
Thu 26 May 2016
SRR1547378_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547378_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2325340
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237131.0197648515915954No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC253.892042E-545.026
CGCGTAA253.892042E-545.031
CGTTTTT131800.042.2173731
CGACGTC556.184564E-1140.90909218
GCGTAAG2450.040.4081651
TAGGGTG16250.039.323085
GGGCGAT35700.039.201687
GGCGATA7450.038.9597328
TAGGGTC11250.038.85
GTAGGGT18550.038.6927224
CGCCGAT356.251046E-638.5714322
ATAGGGT15400.038.1331144
TGCGTTG1550.037.7419361
CGTAAGG4600.037.6630442
CTAGGGT7550.037.5496674
GCGTAAC301.1402812E-437.50000432
GGCGATT12000.037.58
AGGGTAC13200.037.3295446
AGGGCGA18600.037.1370966
CACGACC4000.037.12527
Produced by FastQC (version 0.11.5)