##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547378_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2325340
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.327339227811848	31.0	31.0	33.0	30.0	34.0
2	31.734228542922754	31.0	31.0	34.0	30.0	34.0
3	31.80817342840187	31.0	31.0	34.0	30.0	34.0
4	35.61244463175277	37.0	35.0	37.0	33.0	37.0
5	35.5273977138827	37.0	35.0	37.0	33.0	37.0
6	35.56718372367052	37.0	35.0	37.0	33.0	37.0
7	35.94450832996465	37.0	35.0	37.0	35.0	37.0
8	35.94869094412	37.0	35.0	37.0	35.0	37.0
9	37.72596136478967	39.0	37.0	39.0	35.0	39.0
10	36.886580457051444	39.0	37.0	39.0	32.0	39.0
11	36.68670904039839	39.0	35.0	39.0	32.0	39.0
12	36.20401833710339	38.0	35.0	39.0	32.0	39.0
13	35.95634530864304	38.0	35.0	39.0	31.0	39.0
14	37.03050349626291	39.0	35.0	41.0	31.0	41.0
15	37.22741964615927	39.0	35.0	41.0	32.0	41.0
16	37.345633756783954	39.0	35.0	41.0	32.0	41.0
17	37.265336251902944	39.0	35.0	41.0	32.0	41.0
18	37.12719430276863	39.0	35.0	41.0	32.0	41.0
19	37.04232499333431	38.0	35.0	41.0	32.0	41.0
20	36.869687013512	38.0	35.0	41.0	32.0	41.0
21	36.73765470855875	38.0	35.0	40.0	31.0	41.0
22	36.63940972072901	38.0	35.0	40.0	31.0	41.0
23	36.551682334626335	38.0	35.0	40.0	31.0	41.0
24	36.44306208984493	38.0	35.0	40.0	31.0	41.0
25	36.326273147152676	38.0	35.0	40.0	31.0	41.0
26	36.13675634530864	38.0	35.0	40.0	30.0	41.0
27	36.111015163373956	38.0	35.0	40.0	30.0	41.0
28	36.16984828025149	38.0	35.0	40.0	30.0	41.0
29	36.1969866772171	38.0	35.0	40.0	30.0	41.0
30	36.18005581979409	38.0	35.0	40.0	30.0	41.0
31	36.0673071464818	38.0	35.0	40.0	30.0	41.0
32	35.86715749094756	38.0	35.0	40.0	30.0	41.0
33	35.630379213362346	38.0	34.0	40.0	29.0	41.0
34	35.396400526374634	38.0	34.0	40.0	27.0	41.0
35	35.2227269990625	38.0	34.0	40.0	26.0	41.0
36	34.96514488203876	38.0	34.0	40.0	25.0	41.0
37	34.913021321613186	38.0	34.0	40.0	25.0	41.0
38	34.84911582822297	38.0	34.0	40.0	24.0	41.0
39	34.7288581454755	37.0	34.0	40.0	24.0	41.0
40	34.597222341679064	37.0	34.0	40.0	23.0	41.0
41	34.57610327952042	37.0	34.0	40.0	23.0	41.0
42	34.487530425658186	37.0	33.0	40.0	23.0	41.0
43	34.36629912184885	37.0	33.0	40.0	23.0	41.0
44	34.2805512312178	37.0	33.0	40.0	23.0	41.0
45	34.129984432384084	36.0	33.0	40.0	23.0	41.0
46	34.04335021975281	36.0	33.0	40.0	23.0	41.0
47	34.002104638461475	36.0	33.0	40.0	23.0	41.0
48	33.89698710726174	36.0	33.0	40.0	23.0	41.0
49	33.77858850748708	36.0	33.0	40.0	22.0	41.0
50	33.642205010880126	35.0	33.0	40.0	22.0	41.0
51	33.16873274445887	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	6.0
13	9.0
14	31.0
15	74.0
16	223.0
17	507.0
18	1152.0
19	2174.0
20	4015.0
21	6249.0
22	9462.0
23	13702.0
24	19871.0
25	29099.0
26	40305.0
27	47790.0
28	50656.0
29	55425.0
30	62748.0
31	75493.0
32	93383.0
33	119324.0
34	190390.0
35	262647.0
36	186387.0
37	226888.0
38	324493.0
39	502711.0
40	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.61924707784668	23.603086000326833	29.23017709238219	15.547489829444297
2	32.48927038626609	25.306879854128862	29.1409858343296	13.062863925275444
3	27.67642581299939	25.081321441165592	33.90656850181048	13.33568424402453
4	23.831310690049627	28.19316745078139	33.5348379161757	14.440683942993282
5	22.484625904168855	31.99175174383101	32.106831689129336	13.416790662870806
6	20.731333912460112	41.184127912477315	28.37253906955542	9.711999105507152
7	88.41051201114676	2.790860691339761	7.826898432057247	0.9717288654562343
8	89.21727575322318	2.0005676589229964	7.517997368126812	1.2641592197270077
9	85.07297857517611	3.798283261802575	9.297866118503102	1.8308720445182212
10	40.583527570161785	24.45629456337568	23.018784349815512	11.941393516647029
11	35.239663877110445	24.462272183852683	24.102840874882816	16.19522306415406
12	32.244273955636594	22.08446936792039	27.652343313235917	18.0189133632071
13	22.330626919074202	30.337542036863425	28.972924389551636	18.358906654510736
14	19.286856975754084	31.646210876689	30.219021734456035	18.84791041310088
15	18.926608581970807	24.936525411337694	38.45553768481169	17.68132832187981
16	20.94816241925912	22.934237573860166	37.76540204873266	18.352197958148057
17	21.180515537512797	23.39718062734912	30.03448958001841	25.387814255119682
18	22.286031290047905	24.814908787532143	33.10956677303104	19.789493149388907
19	25.740579872190732	26.029311842569257	28.37365718561587	19.85645109962414
20	27.31914472722269	25.195369279331192	28.885668332372898	18.59981766107322
21	23.92867279623625	27.403734507641893	30.26912193485684	18.39847076126502
22	24.42816964400905	24.17620649023369	29.187645677621337	22.20797818813593
23	21.724177969673253	27.760585548779964	29.181324021433426	21.33391246011336
24	21.34186828592808	25.406005143333875	34.67682145406693	18.57530511667111
25	20.188703587432375	26.724780032167338	32.953245546887764	20.13327083351252
26	20.306277791634773	30.450643776824034	28.776479998623856	20.466598432917337
27	19.250174168078647	31.03726766838398	29.876362166392873	19.836195997144504
28	18.079076608151926	28.204477624777454	34.31356274781322	19.4028830192574
29	18.86515520311008	25.39000748277671	34.51310346013916	21.231733853974042
30	19.807210988500607	29.253485511796125	31.43789725373494	19.50140624596833
31	24.63751537409583	27.31948876293359	28.74680691855815	19.296188944412428
32	26.285102393628456	26.524035194853223	28.53767621079068	18.653186200727635
33	24.607498258319215	27.74398582572871	27.491205587139945	20.15731032881213
34	20.31986720221559	27.655912683736574	29.793621577919787	22.23059853612805
35	20.49283115587398	27.842853088150548	30.637756199093463	21.026559556882006
36	25.460663816904194	27.523157903790413	27.57936473805981	19.43681354124558
37	21.430457481486577	30.922531758796563	28.39292318542665	19.25408757429021
38	21.788469643148957	30.34936826442585	27.327100553037404	20.53506153938779
39	21.165119939449713	30.819579072307707	27.404207556744392	20.61109343149819
40	24.025346831001055	27.64563461687323	27.155426733294917	21.173591818830793
41	19.49895499152812	27.01488814538949	28.5931519691744	24.89300489390799
42	21.444046892067398	27.461790533857418	27.254380004644478	23.839782569430707
43	21.15776617612908	27.679823165644596	28.05628424230435	23.106126415921974
44	20.93354090154558	28.834665038230966	28.43855092158566	21.79324313863779
45	19.802093457300867	32.0324769711096	25.9492375308557	22.21619204073383
46	22.081846095624726	30.78999200116972	26.661262439041174	20.46689946416438
47	20.922058709694067	28.8217206946081	28.124532326455487	22.131688269242346
48	21.611463269887416	27.489313390729958	30.097276097258895	20.801947242123735
49	21.095624725846545	27.146180773564293	30.434173067164373	21.32402143342479
50	20.78164913518023	30.341627460930443	28.416102591448993	20.46062081244033
51	19.530348250148364	31.13114641299767	27.049635752191076	22.288869584662887
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1395.0
1	2232.5
2	3070.0
3	19764.0
4	36458.0
5	24981.5
6	13505.0
7	13778.0
8	14051.0
9	15101.5
10	16152.0
11	17033.5
12	17915.0
13	17869.0
14	17823.0
15	16822.0
16	15821.0
17	14993.5
18	14166.0
19	13460.0
20	12754.0
21	13210.5
22	13667.0
23	14171.5
24	14676.0
25	15809.0
26	20785.0
27	24628.0
28	29559.5
29	34491.0
30	38099.0
31	41707.0
32	49408.5
33	57110.0
34	63434.5
35	69759.0
36	76234.5
37	82710.0
38	90332.0
39	97954.0
40	118872.5
41	139791.0
42	152701.0
43	165611.0
44	170143.0
45	174675.0
46	174398.5
47	174122.0
48	172757.5
49	171393.0
50	164456.0
51	157519.0
52	148154.5
53	138790.0
54	131459.5
55	124129.0
56	114596.0
57	105063.0
58	98389.5
59	91716.0
60	83383.5
61	75051.0
62	67712.0
63	60373.0
64	51910.5
65	43448.0
66	36557.0
67	29666.0
68	25240.0
69	20814.0
70	17603.5
71	14393.0
72	11289.0
73	8185.0
74	7022.5
75	5129.5
76	4399.0
77	3130.0
78	1861.0
79	1434.0
80	1007.0
81	700.0
82	393.0
83	274.5
84	156.0
85	115.0
86	74.0
87	55.5
88	37.0
89	30.0
90	23.0
91	19.5
92	16.0
93	16.0
94	16.0
95	10.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2325340.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.687460218652664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34797922867365	17.4254360317099
2	6.878133981762115	2.983385142160578
3	2.3572645199517064	1.5336924150388198
4	1.3461111068297256	1.167749243170247
5	0.8029552947238209	0.8707030505839695
6	0.5975433518151885	0.7775518602847363
7	0.4771206403473019	0.7243274434921135
8	0.3826397494446993	0.663878747532571
9	0.31153486792537416	0.6080760049369287
>10	3.962248820416666	21.21395687169453
>50	1.70746306920281	26.669437463913066
>100	0.8274018776512923	24.0597618143445
>500	0.0010021820348359418	0.1367117851476995
>1k	4.0087281393437675E-4	0.13453830684646423
>5k	0.0	0.0
>10k+	2.0043640696718837E-4	1.0307938191438468
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23713	1.0197648515915954	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.600892772669803E-5	0.0	0.0	0.017760843575563144	0.0
2	8.600892772669803E-5	0.0	0.0	0.06915117789226521	0.0
3	8.600892772669803E-5	0.0	0.0	0.10815622661632278	0.0
4	8.600892772669803E-5	0.0	0.0	0.19244497578848685	0.0
5	8.600892772669803E-5	0.0	0.0	0.3237376039632914	0.0
6	8.600892772669803E-5	0.0	0.0	0.5746256461420696	0.0
7	8.600892772669803E-5	0.0	0.0	0.6852761316624666	0.0
8	1.2901339159004704E-4	0.0	0.0	0.9367662363353316	0.0
9	1.2901339159004704E-4	0.0	0.0	1.041181074595543	0.0
10	1.2901339159004704E-4	0.0	0.0	1.2264443049188505	0.0
11	1.2901339159004704E-4	0.0	0.0	1.4778053962001256	0.0
12	1.2901339159004704E-4	0.0	0.0	1.671325483585196	0.0
13	1.2901339159004704E-4	0.0	0.0	1.7854593306785245	0.0
14	1.2901339159004704E-4	0.0	0.0	1.842440245297462	0.0
15	1.2901339159004704E-4	0.0	0.0	1.9017003965011567	0.0
16	1.2901339159004704E-4	0.0	0.0	2.013985051648361	0.0
17	1.2901339159004704E-4	0.0	0.0	2.1403321664788804	0.0
18	1.2901339159004704E-4	0.0	0.0	2.288267522168801	0.0
19	1.2901339159004704E-4	0.0	0.0	2.382146266782492	0.0
20	1.2901339159004704E-4	0.0	0.0	2.481916622945462	0.0
21	1.2901339159004704E-4	0.0	0.0	2.621724134965209	0.0
22	1.2901339159004704E-4	0.0	0.0	2.7630798076840377	0.0
23	1.2901339159004704E-4	0.0	0.0	2.941634341644663	0.0
24	1.2901339159004704E-4	0.0	0.0	3.0684115011138156	0.0
25	1.2901339159004704E-4	0.0	0.0	3.183190415165094	0.0
26	1.2901339159004704E-4	0.0	0.0	3.2917766864200506	0.0
27	1.2901339159004704E-4	0.0	0.0	3.405179457627702	0.0
28	1.2901339159004704E-4	0.0	0.0	3.522882675221688	0.0
29	1.2901339159004704E-4	0.0	0.0	3.651681044492418	0.0
30	1.2901339159004704E-4	0.0	0.0	3.7997024091100657	0.0
31	1.2901339159004704E-4	0.0	0.0	3.9356395193821117	0.0
32	1.2901339159004704E-4	0.0	0.0	4.062932732417625	0.0
33	1.2901339159004704E-4	0.0	0.0	4.196934641815821	0.0
34	1.2901339159004704E-4	0.0	0.0	4.333989868148314	0.0
35	1.2901339159004704E-4	0.0	0.0	4.49701979065427	0.0
36	1.2901339159004704E-4	0.0	0.0	4.641385775843532	0.0
37	1.7201785545339606E-4	0.0	0.0	4.7968469127095394	0.0
38	1.7201785545339606E-4	0.0	0.0	4.957124549528241	0.0
39	1.7201785545339606E-4	0.0	0.0	5.161997815373236	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTATC	25	3.892042E-5	45.0	26
CGCGTAA	25	3.892042E-5	45.0	31
CGTTTTT	13180	0.0	42.217373	1
CGACGTC	55	6.184564E-11	40.909092	18
GCGTAAG	245	0.0	40.408165	1
TAGGGTG	1625	0.0	39.32308	5
GGGCGAT	3570	0.0	39.20168	7
GGCGATA	745	0.0	38.959732	8
TAGGGTC	1125	0.0	38.8	5
GTAGGGT	1855	0.0	38.692722	4
CGCCGAT	35	6.251046E-6	38.57143	22
ATAGGGT	1540	0.0	38.133114	4
TGCGTTG	155	0.0	37.741936	1
CGTAAGG	460	0.0	37.663044	2
CTAGGGT	755	0.0	37.549667	4
GCGTAAC	30	1.1402812E-4	37.500004	32
GGCGATT	1200	0.0	37.5	8
AGGGTAC	1320	0.0	37.329544	6
AGGGCGA	1860	0.0	37.137096	6
CACGACC	400	0.0	37.125	27
>>END_MODULE