FastQCFastQC Report
Thu 26 May 2016
SRR1547377_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547377_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1634031
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT934765.72057690459973No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT25540.1563005842606413No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT25240.1544646337798977No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC17250.10556715264275893No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT302.1660653E-645.00000428
CGGTCTA302.1660653E-645.00000431
TCGTTAG502.1827873E-1145.01
TACGAAC207.034162E-445.034
TAGTCCG207.034162E-445.01
TATCCGT207.034162E-445.019
CGTTTTT325750.044.475061
CGTTAGG2150.043.9534872
TACGGGT900.042.54
GTTTTTT354450.040.9754562
GGACCGT556.184564E-1140.9090928
TCGTTGA3200.040.7812524
AACCGTA501.0822987E-940.534
GCGTAAG1000.040.51
CTACGGG2800.040.178573
GCGTTCG451.9292202E-840.044
TCGATCA451.9292202E-840.017
ACGGGTA850.039.7058835
CGAACGT403.4594086E-739.37529
ATTCGAA403.4594086E-739.37526