##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547377_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1634031
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.304120301267233	31.0	31.0	33.0	30.0	34.0
2	31.671118234598975	31.0	31.0	34.0	30.0	34.0
3	31.431371865038056	31.0	31.0	34.0	30.0	34.0
4	35.366573216787195	37.0	35.0	37.0	33.0	37.0
5	35.36192275421947	37.0	35.0	37.0	33.0	37.0
6	35.431431839420426	37.0	35.0	37.0	33.0	37.0
7	35.95711892858826	37.0	35.0	37.0	35.0	37.0
8	35.98808345741298	37.0	35.0	37.0	35.0	37.0
9	37.73143593970983	39.0	38.0	39.0	35.0	39.0
10	37.06783469836252	39.0	37.0	39.0	33.0	39.0
11	36.662317299977786	39.0	35.0	39.0	32.0	39.0
12	36.47244819712723	39.0	35.0	39.0	32.0	39.0
13	36.36409223570422	39.0	35.0	39.0	32.0	39.0
14	37.56000100365293	40.0	36.0	41.0	32.0	41.0
15	37.692544388692745	40.0	36.0	41.0	32.0	41.0
16	37.768089467090896	40.0	36.0	41.0	32.0	41.0
17	37.68940368940369	40.0	36.0	41.0	32.0	41.0
18	37.34959618269176	39.0	36.0	40.0	32.0	41.0
19	37.02002715982744	38.0	36.0	40.0	32.0	41.0
20	36.60914633810497	38.0	35.0	40.0	32.0	41.0
21	36.47696341134287	38.0	35.0	40.0	31.0	41.0
22	36.39081082305048	38.0	35.0	40.0	31.0	41.0
23	36.30547829263949	37.0	35.0	40.0	31.0	41.0
24	36.12497559715819	37.0	35.0	40.0	31.0	41.0
25	35.99145915836358	37.0	35.0	40.0	31.0	41.0
26	35.768735721660114	36.0	35.0	40.0	30.0	41.0
27	35.65555733030769	36.0	35.0	40.0	30.0	41.0
28	35.50676272359582	36.0	34.0	40.0	30.0	41.0
29	35.37920455609471	36.0	34.0	40.0	29.0	41.0
30	35.23857870505517	36.0	34.0	40.0	29.0	41.0
31	35.007310142830825	35.0	34.0	40.0	27.0	41.0
32	34.557918423824276	35.0	33.0	40.0	24.0	41.0
33	33.97446192881286	35.0	33.0	40.0	21.0	41.0
34	33.33756703514193	35.0	33.0	40.0	18.0	41.0
35	32.853797143383446	35.0	32.0	40.0	15.0	41.0
36	32.465336948931814	35.0	31.0	40.0	14.0	41.0
37	32.34570825155704	35.0	31.0	40.0	12.0	41.0
38	32.2453264350554	35.0	31.0	40.0	10.0	41.0
39	32.0755493622826	35.0	31.0	40.0	10.0	41.0
40	31.955866198376896	35.0	31.0	40.0	10.0	41.0
41	31.89264157167153	35.0	31.0	40.0	10.0	41.0
42	31.768367919580474	35.0	30.0	40.0	10.0	41.0
43	31.647193351900913	35.0	30.0	40.0	10.0	41.0
44	31.54144260421008	35.0	30.0	40.0	10.0	41.0
45	31.42232246511847	35.0	30.0	39.0	10.0	41.0
46	31.32636528927542	35.0	29.0	39.0	10.0	41.0
47	31.285309152641535	35.0	29.0	39.0	10.0	41.0
48	31.152259657252525	35.0	29.0	39.0	9.0	41.0
49	31.03910084937189	35.0	28.0	39.0	8.0	41.0
50	30.906447307303228	35.0	28.0	39.0	8.0	41.0
51	30.591421460180378	35.0	27.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	10.0
14	33.0
15	83.0
16	252.0
17	663.0
18	1521.0
19	2859.0
20	4763.0
21	7767.0
22	11583.0
23	17038.0
24	25468.0
25	39380.0
26	57070.0
27	61233.0
28	55392.0
29	50368.0
30	50576.0
31	55667.0
32	64933.0
33	79234.0
34	113072.0
35	168387.0
36	165050.0
37	149808.0
38	178840.0
39	272902.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.5679359816307	21.43863855704084	24.98802042311315	28.005405038215308
2	42.15795171572632	22.154108459386634	24.461469825235874	11.226469999651169
3	22.653486990148902	21.561586040901304	44.546278497776356	11.238648471173436
4	19.70452212962912	23.939570301909818	44.41011217045454	11.945795398006524
5	18.425048239598883	27.706389903251527	42.69894512405211	11.169616733097474
6	17.91789751846813	34.34800196569098	39.11694453777193	8.61715597806896
7	74.70176514399054	2.3352678131565434	21.749158981683948	1.213808061168974
8	75.27959995862992	1.9346022199089246	21.474623186463415	1.311174634997745
9	71.14981294724518	4.6445263278358855	22.41640458473554	1.7892561401833869
10	34.21556873768001	31.931034356141346	26.42183655022457	7.43156035595408
11	20.972429531630674	23.879718316237575	41.48458627773891	13.663265874392835
12	22.212002097879417	22.681699429202993	40.39696921294639	14.709329259971199
13	17.018465377951824	28.005833426660814	40.76685203646687	14.208849158920486
14	13.838660343653212	30.62420480394803	40.0493013902429	15.487833462155859
15	12.891738284035004	28.04053289074687	45.11438277486779	13.95334605035033
16	15.41647618680429	27.64733349612094	43.84133471152016	13.094855605554606
17	15.280432256181186	28.167091077219464	39.10721400022398	17.445262666375363
18	15.763838017760984	29.245834381355067	40.71813815037781	14.27218945050614
19	17.854312433485045	30.832217993416283	36.78877573314093	14.524693839957747
20	18.99670202095309	30.40229958917548	37.1408498369982	13.460148552873234
21	16.71963383803612	30.837970638255946	38.65434621497389	13.788049308734045
22	17.173541995225307	28.854348540511165	38.21353450454734	15.758574959716187
23	14.626589091639019	31.976504729714428	37.82945366397578	15.567452514670773
24	14.301075071403174	30.52059599848473	42.159481674460274	13.018847255651819
25	14.415332389654786	32.00496196216596	39.38340215087719	14.19630349730207
26	14.411476893645226	34.80056375919428	36.56766609691003	14.220293250250455
27	13.39148400489342	34.18925344745601	37.82902527553027	14.590237272120296
28	12.985738948649079	32.685242813630836	40.28375226663387	14.045265971086227
29	13.091612093038627	30.551562363259936	40.253397885352236	16.103427658349197
30	14.331674246082235	33.54618119240088	38.123144542545404	13.999000018971488
31	16.92972777138255	33.30610006786897	35.7486485874503	14.01552357329818
32	17.156284060706316	33.16203915347995	35.50520155370369	14.17647523211004
33	16.720551813276494	34.27511473160546	34.32884688234189	14.675486572776158
34	14.452296192667092	34.00406724229834	34.67467875456463	16.868957810469936
35	15.378472011852896	34.22077059737545	34.320034320034324	16.080723070737335
36	17.798744332267873	34.89248367993019	31.62632777468726	15.682444213114685
37	15.098489563539491	36.91374276253021	33.27121700873484	14.716550665195458
38	15.713900164684757	36.74636527703575	31.872651130853697	15.667083427425796
39	15.473268255008627	37.37836063085707	31.25283424855465	15.895536865579663
40	18.06128525101421	35.331643034924056	30.834176340595743	15.772895373465987
41	15.370638623135058	34.54432627043184	31.849273361398893	18.235761745034214
42	15.949269016316093	35.45220378315956	30.11111784292954	18.487409357594807
43	16.257280308635515	35.86388507929164	30.708291335966088	17.170543276106756
44	15.864142112358945	36.88516313337997	30.362704257140777	16.887990497120313
45	15.347934035523195	37.81384808488946	29.364130790664316	17.47408708892304
46	16.429676058777343	37.190726491725066	29.259542811611283	17.120054637886305
47	15.718796032633408	35.91963677555689	30.533508850199294	17.828058341610408
48	16.34736427889067	35.01867467630663	31.615740460248304	17.018220584554395
49	16.264746507257204	34.79793223017189	31.432329007222016	17.50499225534889
50	15.427308294640676	36.290376375968386	31.473576694689392	16.808738634701545
51	14.683503556542075	36.63100638849569	30.507621948420805	18.177868106541432
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1070.0
1	1628.0
2	2186.0
3	57208.5
4	112231.0
5	69255.0
6	26279.0
7	27840.5
8	29402.0
9	34479.0
10	39556.0
11	45309.0
12	51062.0
13	53339.0
14	55616.0
15	53108.5
16	50601.0
17	45768.5
18	40936.0
19	37338.0
20	33740.0
21	30930.0
22	28120.0
23	26175.5
24	24231.0
25	23965.5
26	24936.0
27	26172.0
28	28106.5
29	30041.0
30	32464.0
31	34887.0
32	38417.5
33	41948.0
34	46252.0
35	50556.0
36	52106.0
37	53656.0
38	59726.0
39	65796.0
40	72693.5
41	79591.0
42	86850.0
43	94109.0
44	98408.0
45	102707.0
46	105251.0
47	107795.0
48	103839.5
49	99884.0
50	92624.5
51	85365.0
52	75345.0
53	65325.0
54	58655.0
55	51985.0
56	46359.5
57	40734.0
58	35186.0
59	29638.0
60	25624.5
61	21611.0
62	17789.5
63	13968.0
64	11351.0
65	8734.0
66	6473.5
67	4213.0
68	3416.5
69	2620.0
70	2126.0
71	1632.0
72	1291.0
73	950.0
74	783.0
75	497.5
76	379.0
77	268.5
78	158.0
79	125.0
80	92.0
81	74.0
82	56.0
83	47.0
84	38.0
85	31.0
86	24.0
87	15.0
88	6.0
89	6.0
90	6.0
91	6.0
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1634031.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.683539772529535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.60530055797389	26.62181209701981
2	8.94904125920746	6.386669393978744
3	4.425899774193807	4.737953118650226
4	2.5351515069987136	3.618527185175071
5	1.681530766714206	3.000148499638923
6	1.1221276977541104	2.4024892999599476
7	0.815728800618545	2.037566377032877
8	0.6274633877072795	1.791209180084706
9	0.5061743468263965	1.6255883193132488
>10	4.436951182151111	33.25729104751788
>50	0.26425574415646247	5.924199064733934
>100	0.027250708181412966	1.6554331006288614
>500	0.002082845011130715	0.5789164369016987
>1k	8.678520879711314E-4	0.572660893933145
>5k	0.0	0.0
>10k+	1.7357041759422627E-4	5.789535985430961
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93476	5.72057690459973	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT	2554	0.1563005842606413	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT	2524	0.1544646337798977	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	1725	0.10556715264275893	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.119834935812112E-5	0.0	0.0	0.05501731607295088	0.0
2	6.119834935812112E-5	0.0	0.0	0.15201669980557284	0.0
3	6.119834935812112E-5	0.0	0.0	0.25024005052535725	0.0
4	6.119834935812112E-5	0.0	0.0	0.3680468730397404	0.0
5	6.119834935812112E-5	0.0	0.0	0.6690815535323381	0.0
6	6.119834935812112E-5	0.0	0.0	1.274333228684156	0.0
7	6.119834935812112E-5	0.0	0.0	1.7532715107608117	0.0
8	6.119834935812112E-5	0.0	0.0	2.3287195897752246	0.0
9	6.119834935812112E-5	0.0	0.0	2.660353444946883	0.0
10	6.119834935812112E-5	0.0	0.0	2.9526979598306275	0.0
11	6.119834935812112E-5	0.0	0.0	3.2340267718299103	0.0
12	6.119834935812112E-5	0.0	0.0	3.4261283904650526	0.0
13	6.119834935812112E-5	0.0	0.0	3.5637634781714667	0.0
14	6.119834935812112E-5	0.0	0.0	3.6539698451253373	0.0
15	6.119834935812112E-5	0.0	0.0	3.7241643518391023	0.0
16	6.119834935812112E-5	0.0	0.0	3.8233668761486164	0.0
17	6.119834935812112E-5	0.0	0.0	3.9384809712912423	0.0
18	6.119834935812112E-5	0.0	0.0	4.074402505215629	0.0
19	6.119834935812112E-5	0.0	0.0	4.167546392938689	0.0
20	6.119834935812112E-5	0.0	0.0	4.258364743386141	0.0
21	6.119834935812112E-5	0.0	0.0	4.372989251733903	0.0
22	6.119834935812112E-5	0.0	0.0	4.497772686075111	0.0
23	6.119834935812112E-5	0.0	0.0	4.637060129214195	0.0
24	6.119834935812112E-5	0.0	0.0	4.756947695606754	0.0
25	6.119834935812112E-5	0.0	0.0	4.862637244948229	0.0
26	6.119834935812112E-5	0.0	0.0	4.965389273520515	0.0
27	6.119834935812112E-5	0.0	0.0	5.074138740329896	0.0
28	6.119834935812112E-5	0.0	0.0	5.184601760921304	0.0
29	6.119834935812112E-5	0.0	0.0	5.304122137217715	0.0
30	6.119834935812112E-5	0.0	0.0	5.462564663705891	0.0
31	6.119834935812112E-5	0.0	0.0	5.593467932982912	0.0
32	6.119834935812112E-5	0.0	0.0	5.726451946138109	0.0
33	6.119834935812112E-5	0.0	0.0	5.859680752690738	0.0
34	6.119834935812112E-5	0.0	0.0	6.005088030765634	0.0
35	6.119834935812112E-5	0.0	0.0	6.164264937446108	0.0
36	6.119834935812112E-5	0.0	0.0	6.317322009190768	0.0
37	6.119834935812112E-5	0.0	0.0	6.470440279284787	0.0
38	6.119834935812112E-5	0.0	0.0	6.6258228883050565	0.0
39	6.119834935812112E-5	0.0	0.0	6.821780002949761	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	30	2.1660653E-6	45.000004	28
CGGTCTA	30	2.1660653E-6	45.000004	31
TCGTTAG	50	2.1827873E-11	45.0	1
TACGAAC	20	7.034162E-4	45.0	34
TAGTCCG	20	7.034162E-4	45.0	1
TATCCGT	20	7.034162E-4	45.0	19
CGTTTTT	32575	0.0	44.47506	1
CGTTAGG	215	0.0	43.953487	2
TACGGGT	90	0.0	42.5	4
GTTTTTT	35445	0.0	40.975456	2
GGACCGT	55	6.184564E-11	40.909092	8
TCGTTGA	320	0.0	40.78125	24
AACCGTA	50	1.0822987E-9	40.5	34
GCGTAAG	100	0.0	40.5	1
CTACGGG	280	0.0	40.17857	3
GCGTTCG	45	1.9292202E-8	40.0	44
TCGATCA	45	1.9292202E-8	40.0	17
ACGGGTA	85	0.0	39.705883	5
CGAACGT	40	3.4594086E-7	39.375	29
ATTCGAA	40	3.4594086E-7	39.375	26
>>END_MODULE