FastQCFastQC Report
Thu 26 May 2016
SRR1547376_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547376_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences714073
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181432.54077664328437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG207.0314895E-445.01
CATAGCG207.0314895E-445.01
GAACGTA207.0314895E-445.040
CAATGCG207.0314895E-445.01
CGTTTTT84050.044.0898251
AGTAGCG603.6379788E-1241.2500041
TCGTAAG501.0804797E-940.51
TATTGCG451.9264917E-840.01
TTAGCGG1300.039.8076932
AGTACGG850.039.7058832
TTTAGCG403.4557706E-739.3751
GCGAGAC3250.038.7692321
TAGGGAT15050.038.720935
ACACGAC3150.038.5714326
GTTAGCG356.2449653E-638.571431
TAATCGT356.2449653E-638.5714321
AGCACGG700.038.571432
GTAGCGG1700.038.382352
ACGGGAC2700.038.3333365
ACGCCGG1000.038.2527
Produced by FastQC (version 0.11.5)