##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547376_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	714073
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.075756960422815	33.0	31.0	34.0	31.0	34.0
2	32.53988597804426	34.0	31.0	34.0	31.0	34.0
3	32.49818155846811	34.0	31.0	34.0	31.0	34.0
4	36.2286320866354	37.0	35.0	37.0	35.0	37.0
5	36.244033873287464	37.0	35.0	37.0	35.0	37.0
6	36.30470414089316	37.0	37.0	37.0	35.0	37.0
7	36.442706838096385	37.0	37.0	37.0	35.0	37.0
8	36.46496646701387	37.0	37.0	37.0	35.0	37.0
9	38.324711899203585	39.0	39.0	39.0	37.0	39.0
10	37.97860862964991	39.0	38.0	39.0	35.0	39.0
11	37.761619610319954	39.0	38.0	39.0	35.0	39.0
12	37.66788969755193	39.0	37.0	39.0	35.0	39.0
13	37.632118844992036	39.0	37.0	39.0	35.0	39.0
14	39.00177993006317	40.0	38.0	41.0	35.0	41.0
15	39.041946691724796	40.0	38.0	41.0	35.0	41.0
16	39.08356568586125	40.0	38.0	41.0	35.0	41.0
17	39.00728216862982	40.0	38.0	41.0	35.0	41.0
18	38.73389695451306	40.0	38.0	41.0	35.0	41.0
19	38.54181715314821	40.0	37.0	41.0	35.0	41.0
20	38.28400037531177	40.0	36.0	41.0	35.0	41.0
21	38.21190690587657	40.0	35.0	41.0	35.0	41.0
22	38.1504832139011	40.0	35.0	41.0	35.0	41.0
23	38.0545392417862	40.0	35.0	41.0	34.0	41.0
24	37.93898522980143	40.0	35.0	41.0	34.0	41.0
25	37.87665686841542	40.0	35.0	41.0	34.0	41.0
26	37.76712885097182	40.0	35.0	41.0	34.0	41.0
27	37.76240944553288	40.0	35.0	41.0	34.0	41.0
28	37.77589686208553	40.0	35.0	41.0	34.0	41.0
29	37.76662610125295	40.0	35.0	41.0	34.0	41.0
30	37.71379256742658	40.0	35.0	41.0	34.0	41.0
31	37.578156575028046	40.0	35.0	41.0	33.0	41.0
32	37.295909521855606	40.0	35.0	41.0	33.0	41.0
33	36.882488204987446	40.0	35.0	41.0	32.0	41.0
34	36.42318082324916	40.0	35.0	41.0	30.0	41.0
35	36.07116919418603	40.0	35.0	41.0	27.0	41.0
36	35.841719263996815	40.0	35.0	41.0	25.0	41.0
37	35.74099286767599	40.0	35.0	41.0	24.0	41.0
38	35.6332867928069	39.0	35.0	41.0	24.0	41.0
39	35.52604285556239	39.0	35.0	41.0	23.0	41.0
40	35.441048744316056	39.0	35.0	41.0	23.0	41.0
41	35.36846652933244	39.0	35.0	41.0	22.0	41.0
42	35.27829367585667	39.0	35.0	41.0	22.0	41.0
43	35.18442092055014	39.0	35.0	41.0	21.0	41.0
44	35.082092447130755	39.0	35.0	41.0	21.0	41.0
45	34.98807264803458	39.0	35.0	41.0	20.0	41.0
46	34.89695311263694	39.0	35.0	41.0	20.0	41.0
47	34.86533729744718	38.0	35.0	41.0	20.0	41.0
48	34.766983207599225	38.0	35.0	40.0	20.0	41.0
49	34.67925828311671	38.0	35.0	40.0	20.0	41.0
50	34.589920078199285	38.0	34.0	40.0	20.0	41.0
51	34.172010424704474	38.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	2.0
13	1.0
14	3.0
15	10.0
16	22.0
17	63.0
18	115.0
19	260.0
20	433.0
21	717.0
22	1200.0
23	2028.0
24	3858.0
25	7617.0
26	13753.0
27	17106.0
28	15343.0
29	12590.0
30	11222.0
31	11144.0
32	12983.0
33	17917.0
34	31589.0
35	51633.0
36	50872.0
37	68950.0
38	124277.0
39	258265.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.010721592890363	22.617155388874806	27.399299511394492	21.972823506840335
2	37.41578242000468	23.16765932894816	26.826108815205167	12.590449435841993
3	25.003045907071126	23.989424050482235	38.66761521581127	12.339914826635372
4	22.21691619764366	26.51745689866442	37.97426873722995	13.291358166461972
5	20.624221893279817	30.224920981468284	36.36630988708438	12.784547238167526
6	19.98605184623981	37.707629332015074	32.914141831437405	9.392176990307714
7	83.81271942784562	2.0205217113656446	13.320766924390085	0.8459919363986595
8	84.62916256461173	1.5824712599412105	13.134651499216465	0.6537146762305814
9	79.92712229702005	4.636920875036586	13.993667314126146	1.4422895138172147
10	31.115866304985623	41.15097476028362	19.335278045802042	8.397880888928723
11	21.26771352508777	25.01592974387773	37.68760336828307	16.028753362751427
12	21.93557241346473	22.88827612863111	37.62332422595449	17.552827231949674
13	20.532214493476157	24.96831556437507	37.76224559673871	16.737224345410063
14	18.00390156188513	26.908873462517136	37.15936605921243	17.9278589163853
15	17.266862071524898	26.298571714656628	39.9540383126095	16.48052790120898
16	21.126831570441677	25.445717734741407	37.88156112890419	15.545889565912729
17	20.472416685688998	25.13426498411227	36.29124753351548	18.102070796683254
18	20.396934207006847	24.82715352632014	37.6520327753605	17.123879491312515
19	21.458170243098394	26.151527925016072	35.308574893603314	17.08172693828222
20	22.858307203885317	26.558068992946097	35.254938920810616	15.328684882357965
21	22.713364039811058	25.300214403849463	36.439131573382554	15.547289982956924
22	22.233721202174006	23.49073554104412	36.37989393241307	17.895649324368797
23	19.23640860248182	26.529640526948928	36.36280884447388	17.87114202609537
24	18.503570363254177	26.64041351514481	38.33697675167665	16.519039369924364
25	19.227305891694545	27.85597550950673	36.18747663054058	16.729241968258147
26	18.9193541836759	29.252331344274324	34.37827785114408	17.450036620905706
27	17.654777592767125	28.461935964530237	36.3586075933413	17.52467884936134
28	17.199221928290246	28.0238855131058	37.42950650703779	17.347386051566158
29	18.064819703307645	26.061201025665444	37.53943924500716	18.33454002601975
30	18.52611707766573	26.768131549575465	37.23680912175646	17.468942251002346
31	19.806518381173913	27.655295747073477	35.17357469054284	17.364611181209764
32	19.94908083627304	27.35112516507416	35.00412422819515	17.69566977045764
33	19.710169688533245	28.22232460826834	33.97425753389359	18.093248169304818
34	18.706630834662562	29.254151886431778	34.04091738519731	17.998299893708346
35	19.03474854811763	29.103467012476315	33.41100979871806	18.450774640687996
36	20.606716680227372	29.96976499601581	31.472832609551126	17.95068571420569
37	19.78145091608281	30.206155393076056	31.689197042879368	18.323196647961762
38	19.552622771061223	30.389049859048022	31.4883772387417	18.569950131149056
39	19.519853012227042	30.331912843644837	31.331250446382935	18.816983697745187
40	20.68962136924376	29.53479546208861	31.350296118183994	18.425287050483636
41	18.902969304258807	29.799614325146027	31.322707902413338	19.974708468181824
42	19.837047472737382	29.765724232676494	30.66829301766066	19.72893527692547
43	19.779070207107676	30.159801588913176	30.72809082544782	19.333037378531326
44	19.435687947870875	30.96658184807436	30.878915741107704	18.718814462947066
45	19.334017670462263	30.649947554381697	30.11288761793262	19.903147157223422
46	19.689443516279148	30.8531480674945	30.590429830003373	18.866978586222977
47	19.320013500020305	30.508085307804667	31.296660145391296	18.875241046783735
48	19.67249847004438	29.837985752156992	31.566520509807823	18.922995267990807
49	19.587353113757278	29.860952591681805	31.47045190057599	19.08124239398493
50	18.873840629739536	30.738173828166026	31.674352622210893	18.71363291988354
51	18.8979278028997	30.445346624224694	31.039823659485794	19.61690191338981
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	375.0
1	530.0
2	685.0
3	13874.5
4	27064.0
5	17694.0
6	8324.0
7	8353.0
8	8382.0
9	8555.0
10	8728.0
11	9129.5
12	9531.0
13	9360.5
14	9190.0
15	8879.5
16	8569.0
17	7998.0
18	7427.0
19	7226.5
20	7026.0
21	7012.5
22	6999.0
23	7482.5
24	7966.0
25	8537.0
26	10630.5
27	12153.0
28	13680.0
29	15207.0
30	17812.5
31	20418.0
32	22661.5
33	24905.0
34	27272.0
35	29639.0
36	31228.0
37	32817.0
38	34678.0
39	36539.0
40	38536.5
41	40534.0
42	42516.0
43	44498.0
44	45086.0
45	45674.0
46	44947.5
47	44221.0
48	44187.0
49	44153.0
50	42347.5
51	40542.0
52	38201.5
53	35861.0
54	33296.5
55	30732.0
56	27744.0
57	24756.0
58	22390.0
59	20024.0
60	18068.0
61	16112.0
62	14195.0
63	12278.0
64	10281.0
65	8284.0
66	6915.0
67	5546.0
68	4379.5
69	3213.0
70	2684.5
71	2156.0
72	1908.5
73	1661.0
74	1428.5
75	1087.0
76	978.0
77	670.0
78	362.0
79	245.5
80	129.0
81	94.5
82	60.0
83	45.5
84	31.0
85	21.5
86	12.0
87	8.0
88	4.0
89	3.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	714073.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.021685180857176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.99585663891381	23.692344573651358
2	10.35898797499951	7.6701438320543405
3	5.864309331738617	6.513198416483699
4	4.0760414808664	6.036076979550031
5	3.0710603165083175	5.684791410459726
6	2.471628232685181	5.49023053887535
7	1.9450827780170248	5.040716958091738
8	1.6243531044319228	4.8109031403861335
9	1.2741224340789021	4.245314367270291
>10	5.293074179522169	27.257528338451593
>50	0.01711580430758711	0.4404612389074098
>100	0.00760702175508294	0.4867884055775574
>500	3.803510877540848E-4	0.07674274834820428
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.803510877540848E-4	2.554759051892606
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18143	2.54077664328437	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0075622520386571124	0.0
2	0.0	0.0	0.0	0.034450259287215734	0.0
3	0.0	0.0	0.0	0.05097518040872572	0.0
4	0.0	0.0	0.0	0.07702293743076688	0.0
5	0.0	0.0	0.0	0.1502647488422052	0.0
6	0.0	0.0	0.0	0.30263012325070404	0.0
7	0.0	0.0	0.0	0.38959602169526086	0.0
8	0.0	0.0	0.0	0.505830636363509	0.0
9	0.0	0.0	0.0	0.589435533901996	0.0
10	0.0	0.0	0.0	0.6827033090454337	0.0
11	0.0	0.0	0.0	0.784373586454046	0.0
12	0.0	0.0	0.0	0.8655977750174002	0.0
13	0.0	0.0	0.0	0.9253955828045592	0.0
14	0.0	0.0	0.0	0.9707690950365019	0.0
15	0.0	0.0	0.0	1.0078801467076894	0.0
16	0.0	0.0	0.0	1.051012991668919	0.0
17	0.0	0.0	0.0	1.0987672128759944	0.0
18	0.0	0.0	0.0	1.1528233107819508	0.0
19	0.0	0.0	0.0	1.19273519654153	0.0
20	0.0	0.0	0.0	1.2334873325276268	0.0
21	0.0	0.0	0.0	1.2777405111242128	0.0
22	0.0	0.0	0.0	1.3386586525467283	0.0
23	0.0	0.0	0.0	1.40755917112116	0.0
24	0.0	0.0	0.0	1.46035489368734	0.0
25	0.0	0.0	0.0	1.5144109915932964	0.0
26	0.0	0.0	0.0	1.5618851293915328	0.0
27	0.0	0.0	0.0	1.6198623950212374	0.0
28	0.0	0.0	0.0	1.6757390350846482	0.0
29	0.0	0.0	0.0	1.742538928092786	0.0
30	0.0	0.0	0.0	1.8174612399572592	0.0
31	0.0	0.0	0.0	1.8911231764819563	0.0
32	0.0	0.0	0.0	1.9625444457359402	0.0
33	0.0	0.0	0.0	2.035646215442959	0.0
34	0.0	0.0	0.0	2.1147697784400195	0.0
35	0.0	0.0	0.0	2.198234634274087	0.0
36	0.0	0.0	0.0	2.269235778414812	0.0
37	0.0	0.0	0.0	2.3529807176577187	0.0
38	0.0	0.0	0.0	2.4412069914420513	0.0
39	0.0	0.0	0.0	2.5581418146323975	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACGCG	20	7.0314895E-4	45.0	1
CATAGCG	20	7.0314895E-4	45.0	1
GAACGTA	20	7.0314895E-4	45.0	40
CAATGCG	20	7.0314895E-4	45.0	1
CGTTTTT	8405	0.0	44.089825	1
AGTAGCG	60	3.6379788E-12	41.250004	1
TCGTAAG	50	1.0804797E-9	40.5	1
TATTGCG	45	1.9264917E-8	40.0	1
TTAGCGG	130	0.0	39.807693	2
AGTACGG	85	0.0	39.705883	2
TTTAGCG	40	3.4557706E-7	39.375	1
GCGAGAC	325	0.0	38.76923	21
TAGGGAT	1505	0.0	38.72093	5
ACACGAC	315	0.0	38.57143	26
GTTAGCG	35	6.2449653E-6	38.57143	1
TAATCGT	35	6.2449653E-6	38.57143	21
AGCACGG	70	0.0	38.57143	2
GTAGCGG	170	0.0	38.38235	2
ACGGGAC	270	0.0	38.333336	5
ACGCCGG	100	0.0	38.25	27
>>END_MODULE