FastQCFastQC Report
Thu 26 May 2016
SRR1547375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547375_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1311318
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193421.475004537419604No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32160.24524943606356353No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT13160.10035704535436867No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGG1350.045.0000042
GGTCGTA351.2121018E-745.08
GATCGGT207.0336513E-445.09
GATCGAC207.0336513E-445.040
GCGAATC253.891108E-545.044
TTAGCGA406.8157533E-945.027
CGTCTTA207.0336513E-445.038
ATTAACG453.8562575E-1045.01
TACGGGA2850.045.04
TACGCCT207.0336513E-445.030
GTCGTGA253.891108E-545.010
GTCGTAC253.891108E-545.020
CGTACTT207.0336513E-445.040
TCGTATC207.0336513E-445.031
GCGATAC502.1827873E-1145.09
ACTCGAT253.891108E-545.045
TCTCCGT207.0336513E-445.014
ATGCGTC253.891108E-545.014
TTACGTA253.891108E-545.020
CGTAAGT406.8157533E-945.031