##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547375_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1311318
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34033087321306	31.0	31.0	33.0	30.0	34.0
2	31.709339763505113	31.0	31.0	34.0	30.0	34.0
3	31.717229535474996	31.0	31.0	34.0	30.0	34.0
4	35.52435793606128	37.0	35.0	37.0	33.0	37.0
5	35.4376772072068	37.0	35.0	37.0	33.0	37.0
6	35.47716800959035	37.0	35.0	37.0	33.0	37.0
7	35.9083395484543	37.0	35.0	37.0	35.0	37.0
8	35.92077894149245	37.0	35.0	37.0	35.0	37.0
9	37.704582717540674	39.0	37.0	39.0	35.0	39.0
10	37.04828119495043	39.0	37.0	39.0	33.0	39.0
11	36.640357258879995	39.0	35.0	39.0	32.0	39.0
12	35.595256833201404	37.0	35.0	39.0	31.0	39.0
13	34.920648538340814	37.0	34.0	39.0	27.0	39.0
14	35.932535052519675	38.0	35.0	40.0	29.0	41.0
15	36.38256547992173	38.0	35.0	41.0	31.0	41.0
16	36.66011371764896	38.0	35.0	41.0	32.0	41.0
17	36.618577644781816	38.0	35.0	40.0	32.0	41.0
18	36.485491696140826	38.0	35.0	40.0	31.0	41.0
19	36.32142546659163	37.0	35.0	40.0	31.0	41.0
20	36.044208956179965	36.0	35.0	40.0	31.0	41.0
21	35.8490976254425	36.0	34.0	40.0	31.0	41.0
22	35.707941933230536	35.0	34.0	40.0	30.0	41.0
23	35.71860143763755	35.0	34.0	40.0	30.0	41.0
24	35.56750917778906	35.0	34.0	40.0	30.0	41.0
25	35.47761717600155	35.0	34.0	40.0	30.0	41.0
26	35.23653301487511	35.0	34.0	40.0	30.0	41.0
27	35.13595100501938	35.0	34.0	40.0	29.0	41.0
28	35.18217854097938	35.0	34.0	40.0	29.0	41.0
29	35.264379044594826	36.0	34.0	40.0	30.0	41.0
30	35.242732121422875	36.0	34.0	40.0	29.0	41.0
31	35.00797289444666	35.0	34.0	40.0	29.0	41.0
32	34.678020129366026	35.0	34.0	40.0	27.0	41.0
33	34.37760863497641	35.0	33.0	40.0	25.0	41.0
34	34.080650917626386	35.0	33.0	40.0	23.0	41.0
35	33.845540898546346	35.0	33.0	40.0	23.0	41.0
36	33.451021033799584	35.0	33.0	40.0	21.0	41.0
37	33.31699709757664	35.0	33.0	40.0	21.0	41.0
38	33.303590738478384	35.0	33.0	40.0	21.0	41.0
39	33.241301499712506	35.0	33.0	40.0	20.0	41.0
40	33.04098395659939	35.0	32.0	39.0	18.0	41.0
41	33.07024764397347	35.0	33.0	39.0	18.0	41.0
42	32.98689257678153	35.0	32.0	39.0	18.0	41.0
43	32.84515350204909	35.0	32.0	39.0	18.0	41.0
44	32.703622614804345	35.0	32.0	39.0	18.0	41.0
45	32.53039537320467	35.0	32.0	39.0	18.0	41.0
46	32.466382677580874	35.0	32.0	39.0	18.0	41.0
47	32.44852964727092	35.0	32.0	39.0	18.0	41.0
48	32.38651951700503	35.0	32.0	39.0	18.0	41.0
49	32.31878079916542	35.0	31.0	38.0	17.0	41.0
50	32.11562641556053	35.0	31.0	38.0	15.0	40.0
51	31.610966218720403	35.0	30.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	3.0
13	8.0
14	19.0
15	64.0
16	209.0
17	418.0
18	993.0
19	1889.0
20	3383.0
21	5338.0
22	7901.0
23	11556.0
24	16616.0
25	23786.0
26	31804.0
27	35987.0
28	36604.0
29	38060.0
30	42162.0
31	48873.0
32	59408.0
33	77116.0
34	137109.0
35	211641.0
36	87870.0
37	95583.0
38	132288.0
39	204561.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.256276509588066	23.806734903356773	28.25668525864817	17.680303328406993
2	32.765507680059294	26.095195825879003	29.619665100303667	11.519631393758036
3	27.031963261390445	24.960002074248962	35.15585083099599	12.8521838333646
4	23.398748434780885	28.511543347990344	34.08723131993918	14.002476897289599
5	21.19684165091915	31.241697284716597	34.434973057641244	13.126488006723008
6	19.81990638426377	40.250191029178275	30.62384562707139	9.306056959486563
7	84.89001142362113	4.039981148737377	9.953420909344644	1.1165865182968586
8	85.55674519834243	3.2623665655470298	9.536588379020191	1.6442998570903473
9	82.50653159645486	4.906208867719348	10.907346654282179	1.6799128815436073
10	51.91852777129575	23.625695674123286	15.291332842224387	9.164443712356576
11	45.82984447708336	19.98554126459028	23.022104478089982	11.162509780236372
12	43.79273372286509	21.009396652833257	23.4541888390154	11.743680785286253
13	19.871381312542038	43.263876496776525	24.528756564006596	12.335985626674843
14	13.916685350159153	44.93196920960438	26.910711208112755	14.24063423212371
15	12.310591328724229	25.14355785553161	49.287205696863765	13.258645118880393
16	13.689890629122761	20.64495416062313	49.58118473169742	16.08397047855669
17	14.695825116409598	21.431567323868048	29.2026037925202	34.670003767202154
18	18.96908301418878	25.523099660036696	37.033351177975135	18.47446614779939
19	26.20111978940272	26.97858185428706	26.985597696363506	19.834700659946712
20	29.715980410548777	24.4342714734336	28.348196242253977	17.50155187376365
21	20.31406569573513	30.82997411764347	30.78116825972037	18.074791926901028
22	22.027685122906878	26.96706672218333	26.446674262078307	24.558573892831486
23	18.041924231955942	32.26219727022736	25.446840507031855	24.249037990784846
24	18.211600847391708	24.751357031627723	42.239868590227545	14.79717353075303
25	14.971349436216084	26.707709342813875	38.74170872358955	19.5792324973805
26	15.604147887850239	36.85528605570884	28.5391491613781	19.00141689506283
27	15.880282280880762	38.77846563533788	28.49049582176101	16.85075626202035
28	13.751050469832643	32.49776179385931	38.69663956416369	15.054548172144361
29	13.318584813142198	26.450410960575542	38.452228978783175	21.77877524749908
30	16.454513703007205	34.68929733291238	33.041642073089825	15.814546890990592
31	26.955704108385607	30.18169505794933	26.528576592405507	16.334024241259556
32	28.111792867938973	28.118656191709412	28.515508823946593	15.254042116405023
33	26.94876452546217	30.407117114231635	25.379351156622576	17.264767203683622
34	18.62972978331724	30.381341520516	27.854875781465672	23.134052914701087
35	19.08713218304027	28.365430810833068	30.82372086709707	21.723716139029587
36	31.0416695263849	27.077489975734338	25.52759895006398	16.353241547816776
37	19.551245388227724	35.13777741173384	30.20861453896004	15.102362661078397
38	18.56529079902815	35.3514555584534	25.671652490090125	20.41160115242832
39	19.275492290962223	35.761195987548405	27.820787940072506	17.142523781416866
40	24.982574783538393	28.35925381943968	25.17795073353679	21.480220663485134
41	16.192029698364546	26.647998426011082	29.578256380222033	27.581715495402335
42	20.278147634669853	27.473656275594475	25.640081200746117	26.608114888989554
43	21.003829734663903	28.061919381873807	26.87250537245733	24.061745511004958
44	18.217244024714066	32.96576421585001	27.801570633515286	21.01542112592064
45	16.573020426776726	40.48468792466816	21.81027027769008	21.132021370865036
46	22.048808908289217	35.83074433508882	24.699577066737437	17.420869689884526
47	20.01940032852443	30.41954735617142	27.179219685842792	22.38183262946135
48	21.449030669906154	27.650730028871717	31.851694249602307	19.04854505161982
49	21.662175002554683	25.244372455804008	31.98362258430068	21.10982995734063
50	19.66845570639616	35.037801662144496	26.692304993906895	18.601437637552447
51	16.998775278002743	35.83112563085385	24.16942343504779	23.000675656095623
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1262.0
1	1648.5
2	2035.0
3	13610.5
4	25186.0
5	17100.0
6	9014.0
7	10057.5
8	11101.0
9	12539.5
10	13978.0
11	14927.0
12	15876.0
13	15640.5
14	15405.0
15	14595.5
16	13786.0
17	12828.5
18	11871.0
19	11060.5
20	10250.0
21	10300.5
22	10351.0
23	9843.5
24	9336.0
25	8960.0
26	10063.5
27	11543.0
28	13098.0
29	14653.0
30	17208.0
31	19763.0
32	22517.5
33	25272.0
34	29547.5
35	33823.0
36	36211.0
37	38599.0
38	44079.0
39	49559.0
40	70902.0
41	92245.0
42	113681.0
43	135117.0
44	132730.5
45	130344.0
46	127175.5
47	124007.0
48	113438.0
49	102869.0
50	97494.5
51	92120.0
52	80910.5
53	69701.0
54	63199.5
55	56698.0
56	48026.0
57	39354.0
58	36783.0
59	34212.0
60	31175.0
61	28138.0
62	24830.0
63	21522.0
64	16525.0
65	11528.0
66	9514.5
67	7501.0
68	6442.0
69	5383.0
70	4351.5
71	3320.0
72	2756.0
73	2192.0
74	1965.0
75	1338.0
76	938.0
77	688.0
78	438.0
79	432.0
80	426.0
81	295.0
82	164.0
83	103.0
84	42.0
85	33.5
86	25.0
87	30.5
88	36.0
89	21.0
90	6.0
91	4.0
92	2.0
93	1.5
94	1.0
95	2.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1311318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.87783221391269
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.08236296489909	19.838267228112542
2	6.91377371715988	3.3017185756660608
3	2.3318540085390516	1.670388562897056
4	1.164992285078711	1.1126996125444881
5	0.7403903545345263	0.8839458329187375
6	0.4977701105967917	0.7131402709158573
7	0.37932419859711813	0.6340207698145217
8	0.29287444785216316	0.559456554044502
9	0.2374084960379367	0.5101920211096088
>10	2.2871163976283757	12.813529783974134
>50	1.029467380296596	17.981737415781026
>100	1.039124631670554	37.74382246434766
>500	0.0025752778975725917	0.40200430003367654
>1k	6.438194743931479E-4	0.3483524833179009
>5k	0.0	0.0
>10k+	3.2190973719657397E-4	1.4867241245222507
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19342	1.475004537419604	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3216	0.24524943606356353	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1316	0.10035704535436867	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.052313778961319835	0.0
2	0.0	0.0	0.0	0.1838608178946678	0.0
3	0.0	0.0	0.0	0.25150268660996034	0.0
4	0.0	0.0	0.0	0.4199591555976506	0.0
5	0.0	0.0	0.0	0.6720719154316497	0.0
6	0.0	0.0	0.0	1.060841077450321	0.0
7	0.0	0.0	0.0	1.2349407237603693	0.0
8	0.0	0.0	0.0	1.6622207580464845	0.0
9	0.0	0.0	0.0	1.8208397962965506	0.0
10	0.0	0.0	0.0	2.1157339409662645	0.0
11	0.0	0.0	0.0	2.5239491870011697	0.0
12	0.0	0.0	0.0	2.8099972699223223	0.0
13	0.0	0.0	0.0	2.952220590276348	0.0
14	0.0	0.0	0.0	3.017345906942481	0.0
15	0.0	0.0	0.0	3.089792102297078	0.0
16	0.0	0.0	0.0	3.2509276925963038	0.0
17	0.0	0.0	0.0	3.428840296556594	0.0
18	0.0	0.0	0.0	3.625055097238046	0.0
19	0.0	0.0	0.0	3.744629449149634	0.0
20	0.0	0.0	0.0	3.8609246574820144	0.0
21	0.0	0.0	0.0	4.025720687125472	0.0
22	0.0	0.0	0.0	4.1979138546104	0.0
23	0.0	0.0	0.0	4.382384745729106	0.0
24	0.0	0.0	0.0	4.531242612394553	0.0
25	0.0	0.0	0.0	4.659129211983668	0.0
26	0.0	0.0	0.0	4.7825165215454986	0.0
27	0.0	0.0	0.0	4.894922513074632	0.0
28	0.0	0.0	0.0	5.011599017172036	0.0
29	0.0	0.0	0.0	5.14009568998519	0.0
30	0.0	0.0	0.0	5.305044237934658	0.0
31	7.62591530048394E-5	0.0	0.0	5.461604279053593	0.0
32	7.62591530048394E-5	0.0	0.0	5.595896647495116	0.0
33	7.62591530048394E-5	0.0	0.0	5.725384689297333	0.0
34	7.62591530048394E-5	0.0	0.0	5.869819525088499	0.0
35	7.62591530048394E-5	0.0	0.0	6.067330731371033	0.0
36	7.62591530048394E-5	0.0	0.0	6.227093656916171	0.0
37	7.62591530048394E-5	0.0	0.0	6.382662329046044	0.0
38	7.62591530048394E-5	0.0	0.0	6.533884229454641	0.0
39	7.62591530048394E-5	0.0	0.0	6.697536371803026	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTGG	135	0.0	45.000004	2
GGTCGTA	35	1.2121018E-7	45.0	8
GATCGGT	20	7.0336513E-4	45.0	9
GATCGAC	20	7.0336513E-4	45.0	40
GCGAATC	25	3.891108E-5	45.0	44
TTAGCGA	40	6.8157533E-9	45.0	27
CGTCTTA	20	7.0336513E-4	45.0	38
ATTAACG	45	3.8562575E-10	45.0	1
TACGGGA	285	0.0	45.0	4
TACGCCT	20	7.0336513E-4	45.0	30
GTCGTGA	25	3.891108E-5	45.0	10
GTCGTAC	25	3.891108E-5	45.0	20
CGTACTT	20	7.0336513E-4	45.0	40
TCGTATC	20	7.0336513E-4	45.0	31
GCGATAC	50	2.1827873E-11	45.0	9
ACTCGAT	25	3.891108E-5	45.0	45
TCTCCGT	20	7.0336513E-4	45.0	14
ATGCGTC	25	3.891108E-5	45.0	14
TTACGTA	25	3.891108E-5	45.0	20
CGTAAGT	40	6.8157533E-9	45.0	31
>>END_MODULE