FastQCFastQC Report
Thu 26 May 2016
SRR1547374_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547374_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3382302
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372201.1004339647967567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT197300.042.4569171
TAGTACG1300.039.807691
AGGGCGA29700.039.5454566
GACCGAT18600.039.072589
GGGCGAT55850.038.6750227
ATAGGGA46300.038.488124
ACACGAC15900.037.526
CGTAAGG5700.037.4999962
CGGGTAT4450.037.415736
GCGAGAC16000.037.4062521
TAAGGGA47150.037.3170744
TACGGGA14950.037.023414
CACGACC16050.037.00934627
CGTTAGG4500.037.02
TATACGG3650.036.9862982
TATTGCG1950.036.9230771
TAGGGAC33550.036.8852465
AACACGT15050.036.62790741
TAGGGAA37550.036.4913445
TCGTAAG1550.036.2903251
Produced by FastQC (version 0.11.5)