FastQCFastQC Report
Thu 26 May 2016
SRR1547373_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547373_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3424659
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250080.730233287460153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT134150.040.9578821
CGCGTAA403.4609002E-739.37531
GGGCGAT61400.038.770367
CATACGA8300.038.7650618
ACGGGAC10600.038.2075465
ACATACG8550.037.6315817
GCGATAA2700.037.59
TAGGGAC37850.037.3910185
TATTGCG2350.037.3404271
TACGGGA12500.037.264
AGGGCGA29250.037.230776
TCGTCCC12000.037.12538
CGTCATA3050.036.88524638
TAAGGGA48000.036.843754
AGGGATT49400.036.7105266
TCGTAAG2150.036.6279071
ACGGGAT15300.036.617655
GACCGAT21550.036.5429239
TAGGGAT51700.036.42655
GTAGGGA47200.036.1811454
Produced by FastQC (version 0.11.5)