##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547373_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3424659
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.303584094066007	31.0	31.0	33.0	30.0	34.0
2	31.7168193972013	31.0	31.0	34.0	30.0	34.0
3	31.820126616985807	31.0	31.0	34.0	30.0	34.0
4	35.60437550132728	37.0	35.0	37.0	33.0	37.0
5	35.49675485938892	37.0	35.0	37.0	33.0	37.0
6	35.53268778000963	37.0	35.0	37.0	33.0	37.0
7	35.91346291703787	37.0	35.0	37.0	35.0	37.0
8	35.90745385160975	37.0	35.0	37.0	35.0	37.0
9	37.65341804833707	39.0	37.0	39.0	35.0	39.0
10	36.95373232780256	39.0	37.0	39.0	32.0	39.0
11	36.638622998669355	39.0	35.0	39.0	32.0	39.0
12	36.24743660609713	38.0	35.0	39.0	32.0	39.0
13	36.08140226515983	38.0	35.0	39.0	31.0	39.0
14	37.12517684242431	39.0	35.0	41.0	31.0	41.0
15	37.284617534183695	39.0	35.0	41.0	32.0	41.0
16	37.3799479013823	39.0	35.0	41.0	32.0	41.0
17	37.30154768693759	39.0	35.0	41.0	32.0	41.0
18	37.16910734762205	39.0	35.0	41.0	32.0	41.0
19	37.105700742760085	39.0	35.0	41.0	32.0	41.0
20	36.96438711124232	39.0	35.0	41.0	31.0	41.0
21	36.83535879046644	39.0	35.0	41.0	31.0	41.0
22	36.74976983109851	38.0	35.0	41.0	31.0	41.0
23	36.66269809636521	38.0	35.0	40.0	31.0	41.0
24	36.54731346974984	38.0	35.0	40.0	31.0	41.0
25	36.44995983541719	38.0	35.0	40.0	30.0	41.0
26	36.220593349586046	38.0	35.0	40.0	30.0	41.0
27	36.22450147591337	38.0	35.0	40.0	30.0	41.0
28	36.266460689954826	38.0	35.0	40.0	30.0	41.0
29	36.27449331451686	38.0	35.0	40.0	30.0	41.0
30	36.248633513584856	38.0	35.0	40.0	30.0	41.0
31	36.18411584919841	38.0	35.0	40.0	30.0	41.0
32	36.02251757036248	38.0	35.0	40.0	30.0	41.0
33	35.84919024054658	38.0	35.0	40.0	29.0	41.0
34	35.636718867484326	38.0	34.0	40.0	27.0	41.0
35	35.52073242912652	38.0	34.0	40.0	27.0	41.0
36	35.29361580233244	38.0	34.0	40.0	26.0	41.0
37	35.258610565314676	38.0	34.0	40.0	26.0	41.0
38	35.17776426791689	38.0	34.0	40.0	26.0	41.0
39	35.027092040404604	38.0	34.0	40.0	25.0	41.0
40	34.89286115785542	38.0	34.0	40.0	24.0	41.0
41	34.86001029591559	38.0	34.0	40.0	24.0	41.0
42	34.77657308362672	37.0	34.0	40.0	24.0	41.0
43	34.66662899868279	37.0	33.0	40.0	24.0	41.0
44	34.56578100184573	37.0	33.0	40.0	24.0	41.0
45	34.419442928478425	37.0	33.0	40.0	23.0	41.0
46	34.34568609604635	37.0	33.0	40.0	23.0	41.0
47	34.29150814723452	37.0	33.0	40.0	23.0	41.0
48	34.172220942289435	36.0	33.0	40.0	23.0	41.0
49	34.033799569533784	36.0	33.0	40.0	23.0	41.0
50	33.92280078104127	36.0	33.0	40.0	23.0	41.0
51	33.43656463315034	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	4.0
12	9.0
13	17.0
14	56.0
15	168.0
16	394.0
17	904.0
18	1786.0
19	3438.0
20	6104.0
21	9292.0
22	13730.0
23	20061.0
24	28230.0
25	39390.0
26	53507.0
27	64060.0
28	71776.0
29	80526.0
30	94299.0
31	112420.0
32	136548.0
33	173747.0
34	262819.0
35	331551.0
36	280417.0
37	354479.0
38	500799.0
39	783881.0
40	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.67586641472918	23.90883296701949	28.655290935535483	14.760009682715856
2	31.162285062541994	25.617149035860216	29.815289639056036	13.40527626254176
3	28.847076453451276	25.82762254577755	31.70049923218633	13.624801768584843
4	25.57498425390674	28.17188514243316	31.550177696523946	14.702952907136154
5	22.99916575635706	32.74498278514737	30.014754753684965	14.24109670481061
6	22.304264453774813	41.388325085796865	26.555607434200017	9.751803026228306
7	89.58497765762957	2.8887255636254587	6.360458077723943	1.165838701021036
8	90.25570137055982	2.217593050870174	6.161022163082515	1.3656834154874982
9	85.3134282858527	4.8409199280862705	7.697350305534069	2.1483014805269662
10	42.0977095821803	33.24976296910145	13.875863261130524	10.776664187587729
11	31.815313583045786	24.61453826497762	26.632753801181373	16.937394350795216
12	30.478859355048193	22.296789256974197	29.00700478500195	18.217346602975653
13	24.510527909494055	26.112468423863515	30.254837050929744	19.122166615712686
14	20.443057250371496	28.425428633916543	31.10738908603747	20.024125029674487
15	19.87059149538684	25.50134188542567	36.049603770769586	18.5784628484179
16	23.563309514903526	23.964108543361544	34.12293019538588	18.349651746349053
17	24.180976850541906	23.825759002575147	29.172101514340554	22.821162632542393
18	23.857908188815294	24.752975405726527	31.48474052453106	19.904375880927123
19	26.74561175287817	25.53060027290308	28.255484706652545	19.4683032675662
20	27.78770090686401	26.15253080671682	27.94030004155158	18.119468244867594
21	26.192155189757578	25.472609097723307	30.07295032877726	18.262285383741855
22	25.437393912795404	23.329826414834294	29.895940004537678	21.33683966783262
23	23.31838001973335	25.92515050403558	29.674049299506898	21.082420176724163
24	22.482121577651963	24.844283766646548	32.954580295439634	19.719014360261856
25	23.054616532624124	26.500156657932948	29.912087597626506	20.53313921181642
26	21.433754426353104	28.592014562617766	28.301503886956336	21.67272712407279
27	21.028137399957192	28.52091259304941	30.000505159783792	20.450444847209607
28	20.413798862894087	27.490182234202003	31.384788967310325	20.711229935593586
29	21.4470696206542	25.690441004491248	31.77241880140475	21.090070573449797
30	22.003446182525032	27.109852396983175	30.73310364623164	20.153597774260152
31	24.708007424972823	26.176941996268827	28.62118535013267	20.493865228625683
32	26.1329376151027	26.000661671716806	27.961178032615802	19.905222680564695
33	25.34153035382501	26.37459671167261	28.01916920779558	20.264703726706802
34	22.113179735559076	26.869974499650912	29.69154009202084	21.325305672769172
35	22.407136009745788	26.99474604624869	29.872813614435774	20.725304329569745
36	24.983246507170495	27.51339622426642	27.4391406560478	20.06421661251529
37	22.392828015869608	29.0938163478466	27.95011123735239	20.563244398931396
38	22.95016817732802	28.889066035479736	27.382667880218147	20.778097906974097
39	22.557778745270696	28.234343915700805	27.478297839288523	21.729579499739973
40	24.11188968011122	26.44628268099101	27.906603255973806	21.535224382923964
41	20.967693425827214	26.36271231675913	28.929800018045594	23.739794239368063
42	22.207291295279326	26.690540576448633	27.828288889492352	23.273879238779685
43	22.150993719374686	26.091356832899276	28.784705280146138	22.972944167579897
44	21.515455991384837	27.340970298064715	28.62953654655836	22.514037163992093
45	21.063469384835106	28.64107054162181	27.341612697789763	22.953847375753323
46	22.01944777567635	28.83980565656318	27.51208222482881	21.62866434293166
47	21.391034844637087	27.189334762964723	29.24641548253417	22.17321490986402
48	22.27953206436028	26.788097734694173	29.78235205315332	21.15001814779223
49	22.48156677788942	26.31526233706772	29.299355059876035	21.903815825166827
50	21.242669708137367	27.913815652886903	28.941859612884084	21.90165502609165
51	20.44708684864683	28.598701359755818	28.309241883644475	22.64496990795288
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1824.0
1	3281.5
2	4739.0
3	22857.5
4	40976.0
5	28235.0
6	15494.0
7	15456.0
8	15418.0
9	16199.5
10	16981.0
11	17318.0
12	17655.0
13	17486.0
14	17317.0
15	16849.0
16	16381.0
17	15956.0
18	15531.0
19	15627.0
20	15723.0
21	16629.5
22	17536.0
23	19514.5
24	21493.0
25	24905.5
26	34529.0
27	40740.0
28	46529.0
29	52318.0
30	60375.5
31	68433.0
32	76859.5
33	85286.0
34	98078.5
35	110871.0
36	118567.5
37	126264.0
38	138819.5
39	151375.0
40	168993.0
41	186611.0
42	206691.0
43	226771.0
44	230229.5
45	233688.0
46	237559.0
47	241430.0
48	240010.0
49	238590.0
50	230377.0
51	222164.0
52	210938.5
53	199713.0
54	189175.0
55	178637.0
56	170630.0
57	162623.0
58	155930.5
59	149238.0
60	139925.5
61	130613.0
62	120330.0
63	110047.0
64	94904.5
65	79762.0
66	68762.0
67	57762.0
68	50535.0
69	43308.0
70	37667.0
71	32026.0
72	26276.0
73	20526.0
74	16800.5
75	10434.0
76	7793.0
77	6375.5
78	4958.0
79	3654.0
80	2350.0
81	1817.5
82	1285.0
83	917.5
84	550.0
85	402.0
86	254.0
87	168.5
88	83.0
89	72.5
90	62.0
91	52.0
92	42.0
93	30.5
94	19.0
95	11.0
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3424659.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.619262932274868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.79304893410524	15.851000701383605
2	5.92045220511781	2.3230981698034574
3	1.8989844418369213	1.1177002520609336
4	1.0717456306922046	0.841074373002685
5	0.6875537533178572	0.6744648933207747
6	0.5427375055567414	0.6388865894834822
7	0.415475414714419	0.570592498222469
8	0.3396090764693793	0.5330303812351835
9	0.30586171674901147	0.5400703297634253
>10	5.150461058129052	25.608050963252616
>50	2.0147781446489126	28.092641337156227
>100	0.8570367332491428	22.15782678934108
>500	0.001954667358224329	0.2540187561062278
>1k	1.503590275557176E-4	0.059824647239975624
>5k	0.0	0.0
>10k+	1.503590275557176E-4	0.737719318627865
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25008	0.730233287460153	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017549192488945615	0.0
2	0.0	0.0	0.0	0.055596776204579784	0.0
3	0.0	0.0	0.0	0.0802707656441123	0.0
4	0.0	0.0	0.0	0.1474015369121422	0.0
5	0.0	0.0	0.0	0.2504190928206283	0.0
6	0.0	0.0	0.0	0.4713461982638271	0.0
7	0.0	0.0	0.0	0.5701589559719669	0.0
8	0.0	0.0	0.0	0.7852460639146847	0.0
9	0.0	0.0	0.0	0.8789780237974059	0.0
10	0.0	0.0	0.0	1.02033516329655	0.0
11	2.9199987502405347E-5	0.0	0.0	1.1788034954721038	0.0
12	2.9199987502405347E-5	0.0	0.0	1.3013266430321968	0.0
13	2.9199987502405347E-5	0.0	0.0	1.3662090152625415	0.0
14	2.9199987502405347E-5	0.0	0.0	1.4050157986532381	0.0
15	2.9199987502405347E-5	0.0	0.0	1.4403185835436463	0.0
16	2.9199987502405347E-5	0.0	0.0	1.5049381558864692	0.0
17	2.9199987502405347E-5	0.0	0.0	1.5788433242550572	0.0
18	2.9199987502405347E-5	0.0	0.0	1.6691880855874994	0.0
19	2.9199987502405347E-5	0.0	0.0	1.7287852600799087	0.0
20	2.9199987502405347E-5	0.0	0.0	1.7915360332225778	0.0
21	2.9199987502405347E-5	0.0	0.0	1.8761575970045485	0.0
22	2.9199987502405347E-5	0.0	0.0	1.9758755543252629	0.0
23	2.9199987502405347E-5	0.0	0.0	2.076673911183566	0.0
24	2.9199987502405347E-5	0.0	0.0	2.163047474215681	0.0
25	2.9199987502405347E-5	0.0	0.0	2.242442240234721	0.0
26	2.9199987502405347E-5	0.0	0.0	2.3143618094531457	0.0
27	2.9199987502405347E-5	0.0	0.0	2.3946909750722627	0.0
28	2.9199987502405347E-5	0.0	0.0	2.4741149410788053	0.0
29	2.9199987502405347E-5	0.0	0.0	2.572781698849433	0.0
30	2.9199987502405347E-5	0.0	0.0	2.6856980505212342	0.0
31	2.9199987502405347E-5	0.0	0.0	2.78579560767948	0.0
32	2.9199987502405347E-5	0.0	0.0	2.8860975647502425	0.0
33	2.9199987502405347E-5	0.0	0.0	2.9848811224708798	0.0
34	5.8399975004810695E-5	0.0	0.0	3.0919282766546976	0.0
35	5.8399975004810695E-5	0.0	0.0	3.213341824689699	0.0
36	5.8399975004810695E-5	0.0	0.0	3.321965778198647	0.0
37	5.8399975004810695E-5	0.0	0.0	3.436488129183081	0.0
38	5.8399975004810695E-5	0.0	0.0	3.5736404704818785	0.0
39	5.8399975004810695E-5	0.0	0.0	3.8130803680016023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	13415	0.0	40.957882	1
CGCGTAA	40	3.4609002E-7	39.375	31
GGGCGAT	6140	0.0	38.77036	7
CATACGA	830	0.0	38.76506	18
ACGGGAC	1060	0.0	38.207546	5
ACATACG	855	0.0	37.63158	17
GCGATAA	270	0.0	37.5	9
TAGGGAC	3785	0.0	37.391018	5
TATTGCG	235	0.0	37.340427	1
TACGGGA	1250	0.0	37.26	4
AGGGCGA	2925	0.0	37.23077	6
TCGTCCC	1200	0.0	37.125	38
CGTCATA	305	0.0	36.885246	38
TAAGGGA	4800	0.0	36.84375	4
AGGGATT	4940	0.0	36.710526	6
TCGTAAG	215	0.0	36.627907	1
ACGGGAT	1530	0.0	36.61765	5
GACCGAT	2155	0.0	36.542923	9
TAGGGAT	5170	0.0	36.4265	5
GTAGGGA	4720	0.0	36.181145	4
>>END_MODULE