FastQCFastQC Report
Thu 26 May 2016
SRR1547372_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547372_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2842171
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246480.8672243858655936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTA351.2127748E-745.016
CGAATAA207.035043E-445.017
CGTTTTT139550.042.0010761
GTTTACG1700.041.029411
CTACGCC451.9299478E-840.013
TCGTAAG1800.040.01
CACGACC4700.039.73404327
AGGGCGA28500.039.710536
TACGGGT2550.039.7058834
GGGCGAT49000.039.581637
ACTTACG403.4606273E-739.37522
CAATACG403.4606273E-739.3751
CGACATA356.251539E-638.57142645
GTATCGC356.251539E-638.57142636
GGCGATT12700.037.9133878
GTTGATC12150.037.7777816
TAAGGGC15100.037.6986774
TACGGGA5700.037.4999964
ACGTATA301.14034505E-437.49999624
CGTTTAG301.14034505E-437.49999630
Produced by FastQC (version 0.11.5)