##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547372_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2842171
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.280113687740815	31.0	31.0	33.0	30.0	34.0
2	31.68651604706402	31.0	31.0	34.0	30.0	34.0
3	31.767356362442655	31.0	31.0	34.0	30.0	34.0
4	35.56045572205191	37.0	35.0	37.0	33.0	37.0
5	35.46054477369588	37.0	35.0	37.0	33.0	37.0
6	35.48524138765753	37.0	35.0	37.0	33.0	37.0
7	35.882054598403826	37.0	35.0	37.0	35.0	37.0
8	35.87860582632079	37.0	35.0	37.0	35.0	37.0
9	37.62446418600429	39.0	37.0	39.0	35.0	39.0
10	36.91111196335477	39.0	37.0	39.0	32.0	39.0
11	36.5864196770708	39.0	35.0	39.0	32.0	39.0
12	35.975572546479434	37.0	35.0	39.0	31.0	39.0
13	35.66289677855414	38.0	35.0	39.0	30.0	39.0
14	36.67634213423471	39.0	35.0	41.0	31.0	41.0
15	36.89814933724959	39.0	35.0	41.0	31.0	41.0
16	37.04768643406748	39.0	35.0	41.0	32.0	41.0
17	36.964944755259275	38.0	35.0	41.0	32.0	41.0
18	36.828820292656566	38.0	35.0	40.0	31.0	41.0
19	36.72953492242374	38.0	35.0	40.0	31.0	41.0
20	36.56468101321138	38.0	35.0	40.0	31.0	41.0
21	36.41313488878748	38.0	35.0	40.0	31.0	41.0
22	36.3040355418446	38.0	35.0	40.0	30.0	41.0
23	36.25210094677625	38.0	35.0	40.0	30.0	41.0
24	36.1212516769751	38.0	35.0	40.0	30.0	41.0
25	36.015987074669326	38.0	35.0	40.0	30.0	41.0
26	35.775779149108196	37.0	34.0	40.0	30.0	41.0
27	35.752746404069285	37.0	34.0	40.0	30.0	41.0
28	35.82348669379851	37.0	34.0	40.0	30.0	41.0
29	35.84635372044821	37.0	34.0	40.0	30.0	41.0
30	35.81515784940456	37.0	35.0	40.0	30.0	41.0
31	35.69086377983591	37.0	34.0	40.0	29.0	41.0
32	35.48271796454189	37.0	34.0	40.0	29.0	41.0
33	35.258686053724425	37.0	34.0	40.0	27.0	41.0
34	35.01644482334103	37.0	34.0	40.0	26.0	41.0
35	34.848946104931755	37.0	34.0	40.0	25.0	41.0
36	34.59207063895874	37.0	33.0	40.0	24.0	41.0
37	34.54386206881993	37.0	33.0	40.0	24.0	41.0
38	34.47033975084539	37.0	33.0	40.0	23.0	41.0
39	34.32733779916831	36.0	33.0	40.0	23.0	41.0
40	34.17417073075477	36.0	33.0	40.0	23.0	41.0
41	34.14964053886976	36.0	33.0	40.0	23.0	41.0
42	34.05330221158403	36.0	33.0	40.0	23.0	41.0
43	33.91794160168406	36.0	33.0	40.0	23.0	41.0
44	33.83199110820566	36.0	33.0	40.0	23.0	41.0
45	33.67862595178123	35.0	33.0	40.0	22.0	41.0
46	33.59212235998467	35.0	33.0	40.0	22.0	41.0
47	33.54773059045357	35.0	33.0	40.0	22.0	41.0
48	33.43790468624161	35.0	32.0	40.0	22.0	41.0
49	33.329358437616875	35.0	32.0	39.0	20.0	41.0
50	33.19100117480616	35.0	32.0	39.0	20.0	41.0
51	32.71170629775619	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	11.0
13	15.0
14	56.0
15	140.0
16	372.0
17	967.0
18	2036.0
19	3733.0
20	6416.0
21	9786.0
22	14234.0
23	20278.0
24	28371.0
25	39585.0
26	52468.0
27	62689.0
28	68578.0
29	74774.0
30	85694.0
31	100275.0
32	120896.0
33	154687.0
34	247264.0
35	327863.0
36	220766.0
37	270644.0
38	374545.0
39	554876.0
40	149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.05154475223342	24.156463492168488	29.3756779588561	15.416313796742
2	31.87165726481623	25.893269616782383	29.100219515293062	13.13485360310833
3	28.09377057186214	25.115378349860016	33.04765969394523	13.74319138433261
4	24.813813102730272	27.89487332043005	32.7000732890456	14.591240287794086
5	22.855450991513177	31.974078969914192	30.774010430758743	14.396459607813886
6	22.115347739456915	40.50766122094694	27.53849082268449	9.83850021691165
7	87.99709799304827	3.1964649558383367	7.515346543188288	1.2910905079251036
8	88.64392747656633	2.4859869444871543	7.281053814144188	1.5890317648023289
9	84.08631992937792	4.603136123758915	8.913538277605394	2.397005669257761
10	45.77455051085948	26.59030719826499	15.916881848417988	11.718260442457543
11	37.629227798045925	23.082073527595632	24.04218465391421	15.246514020444229
12	35.27388042450647	21.004753056730223	26.557022782936002	17.164343735827295
13	23.80451422521727	30.118103379423687	27.962040285401546	18.115342109957496
14	19.809997357653707	31.134122471870974	29.956044164830338	19.09983600564498
15	18.892916717537403	23.769787250661555	40.10212615637835	17.235169875422695
16	21.965427132990943	22.098705531792422	37.7817520479943	18.15411528722234
17	22.285147515754684	22.192612619015534	29.305696244173905	26.216543621055877
18	24.25466307270041	23.868233121793164	32.03262576389668	19.844478041609744
19	27.349339642125685	25.486573467958124	27.213035387385208	19.951051502530987
20	29.521798653212635	24.810118743734982	27.12915584600645	18.538926757045935
21	25.38130182877807	26.58460733010083	29.477290423412246	18.556800417708857
22	25.465990610698654	22.999988389157444	29.386303639013978	22.147717361129928
23	23.87977359560702	26.13685101987178	28.067628583924048	21.915746800597148
24	23.24318276416162	23.517198648497924	34.30180661191744	18.937811975423013
25	21.88812706906094	24.961763384398758	31.620370484393796	21.529739062146508
26	20.839949461168946	29.682872705407238	27.66427495038124	21.812902883042575
27	20.322704017457077	29.802921780568447	29.67185999716414	20.20251420481034
28	18.950935745949135	27.693337241144185	33.154620182951696	20.201106829954988
29	20.60477712284025	25.194367263616442	32.9382362989419	21.26261931460141
30	21.876481042132934	27.479029235046028	30.933149342527244	19.711340380293795
31	26.003220777356468	25.79169233659762	28.082265282419673	20.122821603626242
32	28.18334998140506	25.14912016201699	27.624235135746584	19.043294720831362
33	26.550091461773413	26.731255790028115	26.350631260399183	20.368021487799293
34	21.776029661832453	26.980924089366898	29.204154148360534	22.038892100440123
35	22.026964598541046	26.787621152984816	30.006357815909034	21.179056432565105
36	27.128874371035383	26.74719431026493	26.323820769404797	19.80011054929489
37	22.20102168377624	29.349395233432475	28.46760451781402	19.981978564977265
38	23.042350372303424	29.143038895267033	25.533474235012598	22.281136497416938
39	22.54895993238971	29.34056395621516	26.603677259390796	21.50679885200433
40	24.791928423729605	25.79222010216838	26.809963228813466	22.60588824528855
41	20.589647843145258	24.511649721287	29.18578790649824	25.712914529069504
42	22.955656081213974	25.398155142670863	26.475993175639328	25.170195600475832
43	21.969121491986233	25.698981518001556	27.927981813902118	24.403915176110093
44	21.106154415058068	27.43494321770224	27.979949130435855	23.478953236803836
45	20.68242903048409	30.240052410639613	25.90048241291604	23.177036145960255
46	22.3576625051765	29.16056071221612	27.172995572750548	21.308781209856832
47	22.01827405880927	26.889691014368942	28.40603186789254	22.68600305892925
48	23.003225351324744	24.924925347560016	30.413335439704365	21.65851386141087
49	22.519053216713562	24.832742294534707	30.11405717671456	22.534147312037174
50	21.769063156298476	27.941246321913777	28.635082125600462	21.65460839618728
51	20.11680507611963	29.560853305448546	27.19903200757449	23.123309610857333
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2331.0
1	3623.0
2	4915.0
3	22611.5
4	40308.0
5	27828.5
6	15349.0
7	15718.5
8	16088.0
9	17174.0
10	18260.0
11	18415.0
12	18570.0
13	18486.0
14	18402.0
15	17355.5
16	16309.0
17	15434.5
18	14560.0
19	14616.5
20	14673.0
21	14061.5
22	13450.0
23	14255.0
24	15060.0
25	16701.5
26	23040.0
27	27737.0
28	30776.5
29	33816.0
30	39622.5
31	45429.0
32	50301.0
33	55173.0
34	62369.0
35	69565.0
36	73623.0
37	77681.0
38	92366.5
39	107052.0
40	131434.5
41	155817.0
42	175209.5
43	194602.0
44	202960.0
45	211318.0
46	209932.0
47	208546.0
48	202475.0
49	196404.0
50	194647.0
51	192890.0
52	180607.0
53	168324.0
54	161449.0
55	154574.0
56	148445.0
57	142316.0
58	136810.0
59	131304.0
60	121347.0
61	111390.0
62	102512.0
63	93634.0
64	84666.0
65	75698.0
66	63867.0
67	52036.0
68	44832.5
69	37629.0
70	31085.5
71	24542.0
72	21149.5
73	17757.0
74	15744.5
75	10875.5
76	8019.0
77	6079.0
78	4139.0
79	3262.5
80	2386.0
81	1633.5
82	881.0
83	690.0
84	499.0
85	467.5
86	436.0
87	281.5
88	127.0
89	93.5
90	60.0
91	41.0
92	22.0
93	17.0
94	12.0
95	8.5
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2842171.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.786816689754406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.0302612485824	16.429045590281188
2	6.353956752276324	2.5144915502383776
3	1.9463660284586213	1.1553716344882816
4	0.9476483307121283	0.7500377522461058
5	0.6247716741782069	0.6181121294957572
6	0.4195941186361285	0.4981459145731283
7	0.2920681539347458	0.40453693159752513
8	0.2550613380727826	0.40374815528717006
9	0.22152845898300394	0.3945008708514429
>10	3.0835175016197502	15.647075347295672
>50	1.566765570226982	22.43670712667328
>100	1.2550513374018448	37.307929745997626
>500	0.0025122535178766277	0.3663945205799795
>1k	7.177867193933222E-4	0.19873147898886956
>5k	0.0	0.0
>10k+	1.7944667984833056E-4	0.8751712514056919
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24648	0.8672243858655936	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020758779116386735	0.0
2	0.0	0.0	0.0	0.08377398826460476	0.0
3	0.0	0.0	0.0	0.12733224003763322	0.0
4	0.0	0.0	0.0	0.2563181455303006	0.0
5	0.0	0.0	0.0	0.43793987061299267	0.0
6	0.0	0.0	0.0	0.7676877992210884	0.0
7	0.0	0.0	0.0	0.9037457633618807	0.0
8	7.036874276741265E-5	0.0	0.0	1.2002796453837576	0.0
9	7.036874276741265E-5	0.0	3.518437138370633E-5	1.3157899366364656	0.0
10	7.036874276741265E-5	0.0	3.518437138370633E-5	1.4938228558380195	0.0
11	7.036874276741265E-5	0.0	3.518437138370633E-5	1.7332173187327575	0.0
12	7.036874276741265E-5	0.0	3.518437138370633E-5	1.9073799570821037	0.0
13	7.036874276741265E-5	0.0	3.518437138370633E-5	1.9927020576875916	0.0
14	7.036874276741265E-5	0.0	3.518437138370633E-5	2.0317918942948894	0.0
15	7.036874276741265E-5	0.0	3.518437138370633E-5	2.0777074989506263	0.0
16	7.036874276741265E-5	0.0	3.518437138370633E-5	2.17115718934575	0.0
17	7.036874276741265E-5	0.0	3.518437138370633E-5	2.267491998194338	0.0
18	7.036874276741265E-5	0.0	3.518437138370633E-5	2.3891947388105783	0.0
19	7.036874276741265E-5	0.0	3.518437138370633E-5	2.463750421772652	0.0
20	7.036874276741265E-5	0.0	3.518437138370633E-5	2.5408745638457364	0.0
21	7.036874276741265E-5	0.0	3.518437138370633E-5	2.646533231111006	0.0
22	7.036874276741265E-5	0.0	3.518437138370633E-5	2.7563788385709374	0.0
23	7.036874276741265E-5	0.0	3.518437138370633E-5	2.8755834888189344	0.0
24	7.036874276741265E-5	0.0	3.518437138370633E-5	2.9682943074150008	0.0
25	7.036874276741265E-5	0.0	3.518437138370633E-5	3.0538275142487907	0.0
26	7.036874276741265E-5	0.0	3.518437138370633E-5	3.1398884866533363	0.0
27	7.036874276741265E-5	0.0	3.518437138370633E-5	3.232528936506635	0.0
28	7.036874276741265E-5	0.0	3.518437138370633E-5	3.3185195401684133	0.0
29	7.036874276741265E-5	0.0	3.518437138370633E-5	3.4189357360975112	0.0
30	7.036874276741265E-5	0.0	3.518437138370633E-5	3.5334256805800917	0.0
31	7.036874276741265E-5	0.0	3.518437138370633E-5	3.638943610359827	0.0
32	1.0555311415111899E-4	0.0	3.518437138370633E-5	3.742139371628238	0.0
33	1.0555311415111899E-4	0.0	3.518437138370633E-5	3.843857389298533	0.0
34	1.0555311415111899E-4	0.0	3.518437138370633E-5	3.958136227552811	0.0
35	1.407374855348253E-4	0.0	3.518437138370633E-5	4.091168335754604	0.0
36	1.407374855348253E-4	0.0	3.518437138370633E-5	4.205447174008882	0.0
37	1.407374855348253E-4	0.0	3.518437138370633E-5	4.330457245535191	0.0
38	1.407374855348253E-4	0.0	3.518437138370633E-5	4.464017119307741	0.0
39	1.407374855348253E-4	0.0	3.518437138370633E-5	4.653696065437301	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGTA	35	1.2127748E-7	45.0	16
CGAATAA	20	7.035043E-4	45.0	17
CGTTTTT	13955	0.0	42.001076	1
GTTTACG	170	0.0	41.02941	1
CTACGCC	45	1.9299478E-8	40.0	13
TCGTAAG	180	0.0	40.0	1
CACGACC	470	0.0	39.734043	27
AGGGCGA	2850	0.0	39.71053	6
TACGGGT	255	0.0	39.705883	4
GGGCGAT	4900	0.0	39.58163	7
ACTTACG	40	3.4606273E-7	39.375	22
CAATACG	40	3.4606273E-7	39.375	1
CGACATA	35	6.251539E-6	38.571426	45
GTATCGC	35	6.251539E-6	38.571426	36
GGCGATT	1270	0.0	37.913387	8
GTTGATC	1215	0.0	37.77778	16
TAAGGGC	1510	0.0	37.698677	4
TACGGGA	570	0.0	37.499996	4
ACGTATA	30	1.14034505E-4	37.499996	24
CGTTTAG	30	1.14034505E-4	37.499996	30
>>END_MODULE