FastQCFastQC Report
Thu 26 May 2016
SRR1547371_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547371_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1921418
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286621.491710809412632No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG1250.044.9999961
CGTTTTT151000.043.658941
CGTTAGG2750.043.3636322
GCGATCG603.6379788E-1241.2499969
GTATGCG556.184564E-1140.9090921
TATAGCG556.184564E-1140.9090921
CTATGCG850.039.7058831
TACGGGT2050.039.5121964
AGGGCGA14250.039.3157926
TATCCGG1150.039.1304322
ATAGGGA28600.038.627624
GACCGAT10350.038.478269
TAGGGAT30900.038.3009725
TACTCGC1000.038.2545
AGGGTAC6200.038.104846
GGGCGAT28000.037.8482137
TCGTCCC4900.037.6530638
TAACGGG6400.037.617193
TCGATAG1500.037.5000041
ACGGGAT7500.037.55
Produced by FastQC (version 0.11.5)