##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547371_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1921418
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300165294589725	31.0	31.0	33.0	30.0	34.0
2	31.70358401971877	31.0	31.0	34.0	30.0	34.0
3	31.716434945441335	31.0	31.0	34.0	30.0	34.0
4	35.533497656418334	37.0	35.0	37.0	33.0	37.0
5	35.45781813223359	37.0	35.0	37.0	33.0	37.0
6	35.50222335795751	37.0	35.0	37.0	33.0	37.0
7	35.92825975399418	37.0	35.0	37.0	35.0	37.0
8	35.93487934431758	37.0	35.0	37.0	35.0	37.0
9	37.69613743599779	39.0	37.0	39.0	35.0	39.0
10	36.96160543931617	39.0	37.0	39.0	32.0	39.0
11	36.63685361540279	39.0	35.0	39.0	32.0	39.0
12	36.32822165713031	38.0	35.0	39.0	32.0	39.0
13	36.171220942033436	38.0	35.0	39.0	31.0	39.0
14	37.28574781749729	39.0	35.0	41.0	32.0	41.0
15	37.44359530305223	39.0	35.0	41.0	32.0	41.0
16	37.5344500780153	39.0	35.0	41.0	32.0	41.0
17	37.44101387621017	39.0	35.0	41.0	32.0	41.0
18	37.26630072165453	39.0	36.0	41.0	32.0	41.0
19	37.139984636346696	38.0	35.0	41.0	32.0	41.0
20	36.934088261898246	38.0	35.0	41.0	32.0	41.0
21	36.81051286081425	38.0	35.0	41.0	31.0	41.0
22	36.70910962632806	38.0	35.0	40.0	31.0	41.0
23	36.610627151405886	38.0	35.0	40.0	31.0	41.0
24	36.4804113420401	38.0	35.0	40.0	31.0	41.0
25	36.397214453075804	38.0	35.0	40.0	31.0	41.0
26	36.19623892354501	38.0	35.0	40.0	30.0	41.0
27	36.169777216618144	38.0	35.0	40.0	30.0	41.0
28	36.1871794684967	38.0	35.0	40.0	30.0	41.0
29	36.168368881732135	38.0	35.0	40.0	30.0	41.0
30	36.131615817068436	38.0	35.0	40.0	30.0	41.0
31	36.01780143623095	38.0	35.0	40.0	30.0	41.0
32	35.7677251904583	38.0	35.0	40.0	29.0	41.0
33	35.48654014899413	38.0	34.0	40.0	27.0	41.0
34	35.16397681295793	38.0	34.0	40.0	25.0	41.0
35	34.94353493097285	38.0	34.0	40.0	24.0	41.0
36	34.68287067155611	38.0	34.0	40.0	23.0	41.0
37	34.621986470408835	38.0	34.0	40.0	23.0	41.0
38	34.5496778941386	38.0	33.0	40.0	23.0	41.0
39	34.41509551799764	38.0	33.0	40.0	22.0	41.0
40	34.30605937906275	38.0	33.0	40.0	22.0	41.0
41	34.282075009185924	37.0	33.0	40.0	22.0	41.0
42	34.19111614443083	37.0	33.0	40.0	21.0	41.0
43	34.08434135622753	37.0	33.0	40.0	21.0	41.0
44	33.98752848156934	37.0	33.0	40.0	20.0	41.0
45	33.843928286296894	37.0	33.0	40.0	20.0	41.0
46	33.75933138962995	37.0	33.0	40.0	20.0	41.0
47	33.72362338647811	37.0	33.0	40.0	20.0	41.0
48	33.6141219661729	36.0	33.0	40.0	20.0	41.0
49	33.47655325389894	36.0	32.0	40.0	19.0	41.0
50	33.37106813821875	36.0	32.0	40.0	18.0	41.0
51	32.90230912794613	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	5.0
13	9.0
14	21.0
15	63.0
16	176.0
17	500.0
18	1090.0
19	2111.0
20	3624.0
21	5825.0
22	8922.0
23	12903.0
24	19206.0
25	28362.0
26	39467.0
27	45270.0
28	46035.0
29	47951.0
30	53604.0
31	61886.0
32	74661.0
33	93871.0
34	140333.0
35	187597.0
36	153436.0
37	187299.0
38	270195.0
39	436831.0
40	159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.609850641557433	23.848636788038835	28.244088480486806	18.297424089916923
2	34.02913889637757	25.05555792648971	27.829863153150434	13.08544002398229
3	26.774392662086022	25.01397405457844	35.03959055239412	13.172042730941419
4	22.975115253422214	28.391219401504515	34.77582701942003	13.85783832565324
5	21.162235390737465	32.05549234992073	33.616474915921465	13.165797343420326
6	20.266386595732943	40.04885974837334	30.299133244301863	9.385620411591855
7	86.5315615862868	2.4982070533324867	9.980805842351845	0.9894255180288725
8	87.1182636989973	2.2046738398412002	9.498661925723606	1.1784005354378901
9	82.70464833784216	4.742487059036607	10.784743351004309	1.7681212521169263
10	39.19938295571291	33.3299157184954	17.27031806717747	10.200383258614211
11	27.720933185803403	25.217469597973995	30.817188139176377	16.244409077046225
12	28.005462632285116	23.010245558228352	31.433816067092117	17.55047574239442
13	21.218079564155225	28.917601479740483	32.138035554991156	17.726283401113136
14	17.392779707486866	30.819634249288807	32.8073329176681	18.980253125556228
15	16.818412235130513	27.44369002476296	38.858592976645376	16.879304763461153
16	19.99106909584484	24.577993960710266	38.15354077041019	17.277396173034706
17	19.81021308221324	25.50585036676038	32.095827144327785	22.588109406698596
18	20.442662658515744	26.439067397099436	34.028774582105505	19.089495362279315
19	23.650970272996297	27.629906662683496	30.352947666775265	18.366175397544936
20	25.280704146625045	26.3026577246596	31.21538363854195	17.201254490173405
21	22.419744168109176	27.565527126320248	32.32341947457555	17.691309230995024
22	22.405952270666766	25.159855898091934	31.636739116631574	20.797452714609733
23	20.36376259616596	28.126467015506257	31.795163780083254	19.714606608244537
24	19.63190726848609	26.4812237628668	36.39515191384696	17.49171705480015
25	19.405876285118595	28.21239313881727	33.5084817567026	18.873248819361535
26	19.22809092035153	30.91799910274599	31.09682536543324	18.75708461146924
27	18.54125442771953	30.67614647099174	32.285270565800886	18.497328535487853
28	17.570981431422002	29.310332265025103	35.6015713395003	17.517114964052592
29	18.733768498057163	27.089524507421082	34.79742565126381	19.379281343257947
30	18.7179468496704	29.77826792504286	32.98954209859593	18.514243126690808
31	21.821019684420566	28.73752613954902	30.978735496388605	18.462718679641807
32	22.558860175141486	28.700886532758616	30.645179757866327	18.09507353423357
33	22.112731326551536	29.227789059954677	29.92628360929272	18.733196004201062
34	19.34706555262832	29.576333728527576	30.550510092025785	20.52609062681832
35	19.76545447164542	28.567599554079333	31.16099672221245	20.5059492520628
36	22.19105889504522	29.91191921799421	28.847392915024216	19.04962897193635
37	20.00819186663183	31.596508412016544	29.733509314475036	18.661790406876587
38	20.272996297526095	31.252699828980475	28.501658670835813	19.97264520265762
39	20.258840085811624	30.774042920384844	29.00389191732356	19.963225076479972
40	21.712454031345597	29.195156910156978	29.06285878450186	20.02953027399556
41	18.555358594537992	28.650507073421817	30.40566914643248	22.38846518560771
42	20.171456705412357	29.67022272092798	28.30305534766511	21.855265225994554
43	20.00767141767174	29.950068126768876	28.793942806822876	21.248317648736506
44	19.47910345380339	31.076736035573727	28.885645913590903	20.558514597031984
45	18.980565394932285	32.458580069511164	27.883781665415853	20.677072870140698
46	20.24879542088187	31.647304230521417	27.875870841222472	20.22802950737424
47	19.75343210066732	29.702126242181553	29.85971818729709	20.684723469854035
48	20.59895348123105	28.68537715374791	30.398486950783223	20.31718241423782
49	19.57772853174062	29.566237018701813	29.916447123947005	20.939587325610564
50	19.22528049596704	30.889842814005075	29.573419214351066	20.311457475676818
51	18.18937888580205	31.49606176271899	28.7231097033545	21.59144964812446
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1187.0
1	1836.0
2	2485.0
3	22671.0
4	42857.0
5	29319.0
6	15781.0
7	15830.5
8	15880.0
9	16711.5
10	17543.0
11	18518.5
12	19494.0
13	19228.0
14	18962.0
15	18266.0
16	17570.0
17	17085.5
18	16601.0
19	16006.5
20	15412.0
21	15303.5
22	15195.0
23	15415.0
24	15635.0
25	18425.5
26	24395.5
27	27575.0
28	32154.0
29	36733.0
30	40900.0
31	45067.0
32	51916.5
33	58766.0
34	64819.0
35	70872.0
36	74470.5
37	78069.0
38	84520.5
39	90972.0
40	104596.5
41	118221.0
42	126430.5
43	134640.0
44	138586.5
45	142533.0
46	143466.5
47	144400.0
48	140990.5
49	137581.0
50	127572.0
51	117563.0
52	109765.5
53	101968.0
54	93038.5
55	84109.0
56	78193.0
57	72277.0
58	67997.0
59	63717.0
60	58360.5
61	53004.0
62	45804.5
63	38605.0
64	31449.5
65	24294.0
66	20298.0
67	16302.0
68	13116.0
69	9930.0
70	8499.5
71	7069.0
72	5815.0
73	4561.0
74	3799.5
75	2399.0
76	1760.0
77	1359.5
78	959.0
79	727.0
80	495.0
81	349.5
82	204.0
83	208.5
84	213.0
85	125.0
86	37.0
87	35.5
88	34.0
89	25.5
90	17.0
91	14.5
92	12.0
93	7.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1921418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.955253701550745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.28467148428892	20.318985093172735
2	6.964623083165645	3.615371181184816
3	2.8488573350870414	2.2182844467512375
4	1.6681978064129128	1.7319398915927036
5	1.1300180473340404	1.4664952552942998
6	0.8301764726194042	1.292846457833708
7	0.6444383665570286	1.1708592909300441
8	0.5267087005932657	1.0936686360569876
9	0.4326010301964782	1.0105442541271625
>10	5.078789590588893	30.078266096505118
>50	1.2394874464335763	22.545100416460826
>100	0.34960660379629405	11.66627608524475
>500	0.0014186922762153857	0.22009345016837986
>1k	2.0267032517362653E-4	0.06354514526849973
>5k	0.0	0.0
>10k+	2.0267032517362653E-4	1.5077242994087245
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28662	1.491710809412632	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5613468802727986E-4	0.0	0.0	0.03424554157398338	0.0
2	1.5613468802727986E-4	0.0	0.0	0.09175515166403146	0.0
3	1.5613468802727986E-4	0.0	0.0	0.14010486005647912	0.0
4	1.5613468802727986E-4	0.0	0.0	0.22566666909542848	0.0
5	1.5613468802727986E-4	0.0	0.0	0.37404666761735345	0.0
6	1.5613468802727986E-4	0.0	0.0	0.6740854931097762	0.0
7	1.5613468802727986E-4	0.0	0.0	0.8154394306704735	0.0
8	2.602244800454664E-4	0.0	0.0	1.0989279792320048	0.0
9	2.602244800454664E-4	0.0	0.0	1.2274788723744652	0.0
10	2.602244800454664E-4	0.0	0.0	1.4191081794799465	0.0
11	2.602244800454664E-4	0.0	0.0	1.6538306604809574	0.0
12	2.602244800454664E-4	0.0	0.0	1.8391104902733293	0.0
13	3.122693760545597E-4	0.0	0.0	1.9593862449503439	0.0
14	3.122693760545597E-4	0.0	0.0	2.0264721159060652	0.0
15	3.122693760545597E-4	0.0	0.0	2.0901230237251864	0.0
16	3.122693760545597E-4	0.0	0.0	2.189424685310536	0.0
17	3.122693760545597E-4	0.0	0.0	2.3166744560527692	0.0
18	3.122693760545597E-4	0.0	0.0	2.4624001648782308	0.0
19	3.122693760545597E-4	0.0	0.0	2.563054993759817	0.0
20	3.122693760545597E-4	0.0	0.0	2.667977504114149	0.0
21	3.122693760545597E-4	0.0	0.0	2.8004317644572914	0.0
22	3.122693760545597E-4	0.0	0.0	2.9513099179876527	0.0
23	3.122693760545597E-4	0.0	0.0	3.120403785121197	0.0
24	3.122693760545597E-4	0.0	0.0	3.252753955672321	0.0
25	3.122693760545597E-4	0.0	0.0	3.3744869674375906	0.0
26	3.122693760545597E-4	0.0	0.0	3.490390950849841	0.0
27	3.122693760545597E-4	0.0	0.0	3.607387877078283	0.0
28	3.122693760545597E-4	0.0	0.0	3.7338049294843705	0.0
29	3.122693760545597E-4	0.0	0.0	3.8711514100523674	0.0
30	3.122693760545597E-4	0.0	0.0	4.028222906207811	0.0
31	3.122693760545597E-4	0.0	0.0	4.182588067770782	0.0
32	3.122693760545597E-4	0.0	0.0	4.329875123476516	0.0
33	3.122693760545597E-4	0.0	0.0	4.4797123790866955	0.0
34	3.122693760545597E-4	0.0	0.0	4.632255969289348	0.0
35	3.122693760545597E-4	0.0	0.0	4.798175097766337	0.0
36	3.122693760545597E-4	0.0	0.0	4.953997516417562	0.0
37	3.122693760545597E-4	0.0	0.0	5.128712232320089	0.0
38	3.122693760545597E-4	0.0	0.0	5.304103531870733	0.0
39	3.122693760545597E-4	0.0	0.0	5.507442940578261	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAG	125	0.0	44.999996	1
CGTTTTT	15100	0.0	43.65894	1
CGTTAGG	275	0.0	43.363632	2
GCGATCG	60	3.6379788E-12	41.249996	9
GTATGCG	55	6.184564E-11	40.909092	1
TATAGCG	55	6.184564E-11	40.909092	1
CTATGCG	85	0.0	39.705883	1
TACGGGT	205	0.0	39.512196	4
AGGGCGA	1425	0.0	39.315792	6
TATCCGG	115	0.0	39.130432	2
ATAGGGA	2860	0.0	38.62762	4
GACCGAT	1035	0.0	38.47826	9
TAGGGAT	3090	0.0	38.300972	5
TACTCGC	100	0.0	38.25	45
AGGGTAC	620	0.0	38.10484	6
GGGCGAT	2800	0.0	37.848213	7
TCGTCCC	490	0.0	37.65306	38
TAACGGG	640	0.0	37.61719	3
TCGATAG	150	0.0	37.500004	1
ACGGGAT	750	0.0	37.5	5
>>END_MODULE