FastQCFastQC Report
Thu 26 May 2016
SRR1547370_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547370_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2475621
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208080.8405163795265915No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAC302.1665219E-644.99999618
CGTTTTT125250.042.6467061
GCGATAC1200.041.2499969
TAACGGG7800.039.8076933
AGGGCGA23050.039.3383946
GGGCGAT49400.038.987857
ACGGGAT14800.038.766895
ATTACGG1800.038.752
TGGGCGA17100.038.684216
CACGACC8950.038.46368827
GACCGAT15950.038.3699049
TAGGGAC24700.038.1680155
AACACGT8550.038.15789441
GGCGATT12350.037.7125478
GACGGGA14300.037.6048974
CGTACCG301.1403026E-437.49999620
TTACGGG8350.037.455093
ACGGGTA2350.037.3404275
CGTAAGG4000.037.1252
CGAGACA9350.037.05882322
Produced by FastQC (version 0.11.5)