##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547370_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2475621
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255287461206702	31.0	31.0	33.0	30.0	34.0
2	31.660132144621493	31.0	31.0	34.0	30.0	34.0
3	31.74190152693001	31.0	31.0	34.0	30.0	34.0
4	35.531148750151985	37.0	35.0	37.0	33.0	37.0
5	35.429186858570034	37.0	35.0	37.0	33.0	37.0
6	35.46562054530964	37.0	35.0	37.0	33.0	37.0
7	35.89906936481796	37.0	35.0	37.0	35.0	37.0
8	35.90346664533868	37.0	35.0	37.0	35.0	37.0
9	37.64475660854388	39.0	37.0	39.0	35.0	39.0
10	36.869494159243274	39.0	37.0	39.0	32.0	39.0
11	36.602036822276105	39.0	35.0	39.0	32.0	39.0
12	36.250371522943134	38.0	35.0	39.0	32.0	39.0
13	36.09174142568673	38.0	35.0	39.0	31.0	39.0
14	37.14621906988186	39.0	35.0	41.0	31.0	41.0
15	37.28465908149915	39.0	35.0	41.0	32.0	41.0
16	37.36580357009413	39.0	35.0	41.0	32.0	41.0
17	37.27955975490594	39.0	35.0	41.0	32.0	41.0
18	37.155472505686454	39.0	35.0	41.0	32.0	41.0
19	37.092049227244395	39.0	35.0	41.0	32.0	41.0
20	36.93329027343039	39.0	35.0	41.0	31.0	41.0
21	36.81498379598493	38.0	35.0	41.0	31.0	41.0
22	36.731912114172566	38.0	35.0	40.0	31.0	41.0
23	36.63613614523386	38.0	35.0	40.0	31.0	41.0
24	36.51448828394977	38.0	35.0	40.0	31.0	41.0
25	36.41681582116164	38.0	35.0	40.0	30.0	41.0
26	36.18838909509977	38.0	35.0	40.0	30.0	41.0
27	36.18615975547146	38.0	35.0	40.0	30.0	41.0
28	36.237041938164204	38.0	35.0	40.0	30.0	41.0
29	36.25770099704276	38.0	35.0	40.0	30.0	41.0
30	36.23081077434713	38.0	35.0	40.0	30.0	41.0
31	36.14108944785975	38.0	35.0	40.0	30.0	41.0
32	35.96201397548332	38.0	35.0	40.0	29.0	41.0
33	35.78227361942721	38.0	35.0	40.0	29.0	41.0
34	35.561753596370366	38.0	34.0	40.0	27.0	41.0
35	35.41724884382545	38.0	34.0	40.0	26.0	41.0
36	35.17399795849203	38.0	34.0	40.0	25.0	41.0
37	35.13800416138012	38.0	34.0	40.0	25.0	41.0
38	35.05936167127359	38.0	34.0	40.0	25.0	41.0
39	34.92258508067269	38.0	34.0	40.0	24.0	41.0
40	34.81654380860398	38.0	34.0	40.0	24.0	41.0
41	34.778248366773425	38.0	34.0	40.0	24.0	41.0
42	34.69215764448597	38.0	33.0	40.0	23.0	41.0
43	34.59535041914736	38.0	33.0	40.0	23.0	41.0
44	34.51595417876969	37.0	33.0	40.0	23.0	41.0
45	34.37010915645004	37.0	33.0	40.0	23.0	41.0
46	34.282282304116826	37.0	33.0	40.0	23.0	41.0
47	34.24663508671158	37.0	33.0	40.0	23.0	41.0
48	34.12510154017921	37.0	33.0	40.0	23.0	41.0
49	33.998541780022066	37.0	33.0	40.0	23.0	41.0
50	33.88435669272477	36.0	33.0	40.0	22.0	41.0
51	33.41708605638747	36.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	11.0
13	10.0
14	31.0
15	83.0
16	260.0
17	567.0
18	1400.0
19	2513.0
20	4282.0
21	6853.0
22	10363.0
23	15109.0
24	21532.0
25	31218.0
26	42062.0
27	49642.0
28	54083.0
29	59719.0
30	68274.0
31	82145.0
32	98549.0
33	123031.0
34	182839.0
35	235772.0
36	199523.0
37	249415.0
38	362922.0
39	573223.0
40	184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.42165137555385	24.123765309794997	28.881763404010552	15.572819910640604
2	31.430538034699175	25.72530286340276	29.25629569308065	13.587863408817425
3	27.597116036743913	25.225024347426363	33.73577781090078	13.442081804928947
4	24.158665643892988	28.562247613831033	32.88827328577355	14.39081345650243
5	22.199722817022476	32.24960525056137	31.593648623921027	13.95702330849512
6	21.217181466791565	40.468593536732804	28.096950219763038	10.217274776712589
7	89.0388310650136	2.7274368734147916	7.129483874955012	1.1042481866166105
8	90.05594959810084	2.0119396305007915	6.763717063314618	1.1683937080837494
9	85.33422523076028	4.757028640490608	8.06399687189598	1.8447492568531292
10	37.08083749491542	35.652912945883074	15.374849381225964	11.891400177975546
11	29.96565306240333	25.553547978466817	27.92563966778437	16.555159291345483
12	28.40386311151828	23.600381480040767	29.505889633348563	18.48986577509239
13	23.37284261201533	28.482510044954378	30.16148271484205	17.98316462818824
14	20.32245646647851	30.542195271408666	30.779792221830398	18.355556040282416
15	19.542086611803665	26.628672159429893	36.516453851377086	17.31278737738935
16	22.69737572916048	24.691703616991454	34.795996640842844	17.81492401300522
17	21.860454407197224	24.888825874396765	30.38538613139895	22.865333587007058
18	22.762409916542154	24.94533694777997	32.61420063895079	19.678052496727084
19	25.140762661166633	26.807940310734157	28.666140738020886	19.385156290078328
20	26.530393788063684	26.915226522961312	28.50238384631573	18.051995842659277
21	24.11673677028915	26.910662011673033	30.648875575057733	18.323725642980087
22	23.86617337629629	24.75314274680979	29.87795789420109	21.502725982692827
23	21.718994951165786	27.855677423967563	29.759078631179815	20.666248993686835
24	20.860907222874584	27.111338932736473	33.52710289660655	18.500650947782397
25	21.481680758080497	27.630117857297222	31.229053235531612	19.659148149090672
26	20.374524210289056	30.426507126898667	28.725358203052892	20.47361045975939
27	19.778188987732776	29.226848536185468	30.911233989370746	20.08372848671101
28	18.595334261585275	29.03804742325259	32.97100000363545	19.395618311526682
29	19.556466842057002	26.544006534118108	32.91848792686764	20.98103869695725
30	20.746471289425966	27.836732682425946	31.896239367819224	19.520556660328864
31	23.614438559052456	27.658918711709102	29.648399330915353	19.07824339832309
32	24.16722915179666	27.36141759986686	29.025242555302285	19.446110693034193
33	23.16291548665971	27.658959105614304	28.931367119603525	20.246758288122454
34	20.4077683942736	28.692679533741234	29.88494603980173	21.01460603218344
35	20.42699589315166	28.05243613622602	30.278705827749885	21.241862142872435
36	23.80408794399466	28.893316060899465	27.807204737720355	19.49539125738552
37	21.153641853902517	30.353596128001826	28.012324988356458	20.480437029739203
38	21.34559369144146	30.3144544338572	27.873450742258203	20.46650113244313
39	20.937978794007645	29.491267039663988	28.322994513295857	21.24775965303251
40	23.107131503570216	27.76434680429678	27.743947882167745	21.384573809965257
41	20.064581775643365	27.43517687077303	28.865080721160467	23.635160632423137
42	21.44657845445648	27.928628816769614	27.793753567286757	22.83103916148716
43	21.12750699723423	28.062292249096288	28.41299213409484	22.397208619574645
44	21.043810825647384	29.0186179548485	28.631038434396867	21.30653278510725
45	20.458462745307138	29.84657182985602	27.394863753377436	22.300101671459405
46	21.749775106932763	29.809369043161293	27.45440436965109	20.986451480254853
47	21.111107071720593	28.83110944688222	28.54742305062043	21.51036043077676
48	21.659615910512958	27.59085498143698	29.789172090558285	20.960357017491773
49	21.45162769260723	27.492374640544735	29.43289784664131	21.62309982020673
50	20.54676382208747	29.482420774423872	28.85756745479215	21.113247948696507
51	20.070519679708646	29.711211853510694	28.00715456849009	22.21111389829057
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1262.0
1	1781.0
2	2300.0
3	17483.5
4	32667.0
5	22611.5
6	12556.0
7	12706.0
8	12856.0
9	13679.0
10	14502.0
11	15445.0
12	16388.0
13	16044.0
14	15700.0
15	15192.5
16	14685.0
17	14000.5
18	13316.0
19	13322.0
20	13328.0
21	14750.0
22	16172.0
23	15944.0
24	15716.0
25	18209.5
26	23474.5
27	26246.0
28	30862.0
29	35478.0
30	41638.0
31	47798.0
32	56399.5
33	65001.0
34	72618.0
35	80235.0
36	89985.0
37	99735.0
38	109046.0
39	118357.0
40	132352.0
41	146347.0
42	161883.5
43	177420.0
44	184772.0
45	192124.0
46	195918.0
47	199712.0
48	197831.5
49	195951.0
50	188931.0
51	181911.0
52	169131.5
53	156352.0
54	145140.0
55	133928.0
56	121837.0
57	109746.0
58	99456.0
59	89166.0
60	78935.5
61	68705.0
62	60314.5
63	51924.0
64	43194.5
65	34465.0
66	28166.5
67	21868.0
68	17930.0
69	13992.0
70	11972.0
71	9952.0
72	8296.0
73	6640.0
74	5831.5
75	3965.5
76	2908.0
77	2101.0
78	1294.0
79	989.0
80	684.0
81	483.5
82	283.0
83	204.5
84	126.0
85	85.0
86	44.0
87	35.0
88	26.0
89	23.0
90	20.0
91	13.5
92	7.0
93	4.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2475621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.615796761332803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.41522132840511	17.960385053194724
2	6.304324908798099	2.8515466170957158
3	2.2938568155298	1.556321986188601
4	1.2743174537554471	1.1527881817420922
5	0.8954109579127706	1.012521612101277
6	0.6439597768666498	0.8738198061653626
7	0.517919482966997	0.8199213235861319
8	0.42660130041759114	0.7718342646691616
9	0.36735875377730054	0.7477299822531538
>10	5.737607997641663	31.858188235314135
>50	1.6762121412470472	26.539957398467408
>100	0.445585512965667	12.719328615667777
>500	0.0010823798104849698	0.1524218374191542
>1k	3.607932701616566E-4	0.13430746487790565
>5k	0.0	0.0
>10k+	1.803966350808283E-4	0.8489276212574304
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20808	0.8405163795265915	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.03939052060069E-5	0.0	0.0	0.02787179459214476	0.0
2	4.03939052060069E-5	0.0	0.0	0.0896340756521293	0.0
3	4.03939052060069E-5	0.0	0.0	0.13148216144555244	0.0
4	4.03939052060069E-5	0.0	0.0	0.21804630030202524	0.0
5	4.03939052060069E-5	0.0	0.0	0.37166432180046943	0.0
6	4.03939052060069E-5	0.0	0.0	0.6742954596038732	0.0
7	4.03939052060069E-5	0.0	0.0	0.8252878772639269	0.0
8	8.07878104120138E-5	0.0	0.0	1.1387849755677464	0.0
9	8.07878104120138E-5	0.0	0.0	1.2761242532681698	0.0
10	8.07878104120138E-5	0.0	0.0	1.4771243255732602	0.0
11	8.07878104120138E-5	0.0	0.0	1.6951706258752854	0.0
12	8.07878104120138E-5	0.0	0.0	1.8632496654374802	0.0
13	8.07878104120138E-5	0.0	0.0	1.9644363979785273	0.0
14	8.07878104120138E-5	0.0	0.0	2.0179179284712805	0.0
15	8.07878104120138E-5	0.0	0.0	2.072611276120214	0.0
16	8.07878104120138E-5	0.0	0.0	2.1673349838283	0.0
17	8.07878104120138E-5	0.0	0.0	2.2824576136654198	0.0
18	8.07878104120138E-5	0.0	0.0	2.419716103555431	0.0
19	8.07878104120138E-5	0.0	0.0	2.5155708406092856	0.0
20	8.07878104120138E-5	0.0	0.0	2.6103349422225777	0.0
21	8.07878104120138E-5	0.0	0.0	2.7298605077271523	0.0
22	1.211817156180207E-4	0.0	0.0	2.8634835461486228	0.0
23	1.211817156180207E-4	0.0	0.0	3.016697628595007	0.0
24	1.211817156180207E-4	0.0	0.0	3.1382024954546757	0.0
25	1.211817156180207E-4	0.0	0.0	3.2494473103920187	0.0
26	1.211817156180207E-4	0.0	0.0	3.355198554221345	0.0
27	1.211817156180207E-4	0.0	0.0	3.4677763680304863	0.0
28	1.211817156180207E-4	0.0	0.0	3.5810812721333356	0.0
29	1.211817156180207E-4	0.0	0.0	3.712482645768476	0.0
30	1.211817156180207E-4	0.0	0.0	3.8700592699771086	0.0
31	1.211817156180207E-4	0.0	0.0	4.010589666188807	0.0
32	1.211817156180207E-4	0.0	0.0	4.163763354729985	0.0
33	1.211817156180207E-4	0.0	0.0	4.307444475547752	0.0
34	1.211817156180207E-4	0.0	0.0	4.449227082820836	0.0
35	1.211817156180207E-4	0.0	0.0	4.60405692147546	0.0
36	1.211817156180207E-4	0.0	0.0	4.752181371865888	0.0
37	1.211817156180207E-4	0.0	0.0	4.905557029933096	0.0
38	1.211817156180207E-4	0.0	0.0	5.08324981893432	0.0
39	1.211817156180207E-4	0.0	0.0	5.338822057172726	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGAC	30	2.1665219E-6	44.999996	18
CGTTTTT	12525	0.0	42.646706	1
GCGATAC	120	0.0	41.249996	9
TAACGGG	780	0.0	39.807693	3
AGGGCGA	2305	0.0	39.338394	6
GGGCGAT	4940	0.0	38.98785	7
ACGGGAT	1480	0.0	38.76689	5
ATTACGG	180	0.0	38.75	2
TGGGCGA	1710	0.0	38.68421	6
CACGACC	895	0.0	38.463688	27
GACCGAT	1595	0.0	38.369904	9
TAGGGAC	2470	0.0	38.168015	5
AACACGT	855	0.0	38.157894	41
GGCGATT	1235	0.0	37.712547	8
GACGGGA	1430	0.0	37.604897	4
CGTACCG	30	1.1403026E-4	37.499996	20
TTACGGG	835	0.0	37.45509	3
ACGGGTA	235	0.0	37.340427	5
CGTAAGG	400	0.0	37.125	2
CGAGACA	935	0.0	37.058823	22
>>END_MODULE