FastQCFastQC Report
Thu 26 May 2016
SRR1547369_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547369_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2782426
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT478001.7179252925324877No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27910.10030814835686555No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG800.045.01
GTCGACG207.035017E-445.01
TCCGATT551.8189894E-1245.012
CTACGCT207.035017E-445.018
CGTCGTC207.035017E-445.041
TACGTAG453.8562575E-1045.01
GTCGTAA253.892241E-544.9999969
CGTTTTT299200.043.7667161
CGTTGAT7200.041.2525
GTTTACG1150.041.0869561
TTAATCG7300.040.99315320
AGGGCGA25050.040.9580846
TACGGGA6100.040.5737724
CCCGTAT501.0822987E-940.49999645
CGTTAGG3950.040.443042
AATCGTT7400.040.4391922
TAATCGT7400.040.4391921
TCGTTGA7350.040.40816524
CGTAAGG4650.040.1612932
CTTCGCA403.460591E-739.37524
Produced by FastQC (version 0.11.5)